Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/44722 |
Resumo: | The species Staphylococcus argenteus was separated recently from Staphylococcus aureus (Tong S.Y., F. Schaumburg, M.J. Ellington, J. Corander, B. Pichon, F. Leendertz, S.D. Bentley, J. Parkhill, D.C. Holt, G. Peters, and P.M. Giffard, 2015). The objective of this work was to characterise the genome of a non-human S. argenteus strain, which had been isolated from the faeces of a wild-living western lowland gorilla in Gabon, and analyse the spectrum of this species in matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The full genome sequence revealed a scarcity of virulence genes and absence of resistance genes, indicating a decreased virulence potential compared to S. aureus and the human methicillin-resistant S. argenteus isolate MSHR1132T. Spectra obtained by MALDI-TOF MS and the analysis of available sequences in the genome databases identified several MALDI-TOF MS signals that clearly differentiate S. argenteus, the closely related Staphylococcus schweitzeri and S. aureus. In conclusion, in the absence of biochemical tests that identify the three species, mass spectrometry should be employed as method of choice. |
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Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great apeStaphylococcus argenteusStaphylococcus aureusStaphylococcus schweitzeriGorilla gorilla ssp. gorillaMALDI-TOF MSVirulence determinantsScience & TechnologyThe species Staphylococcus argenteus was separated recently from Staphylococcus aureus (Tong S.Y., F. Schaumburg, M.J. Ellington, J. Corander, B. Pichon, F. Leendertz, S.D. Bentley, J. Parkhill, D.C. Holt, G. Peters, and P.M. Giffard, 2015). The objective of this work was to characterise the genome of a non-human S. argenteus strain, which had been isolated from the faeces of a wild-living western lowland gorilla in Gabon, and analyse the spectrum of this species in matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The full genome sequence revealed a scarcity of virulence genes and absence of resistance genes, indicating a decreased virulence potential compared to S. aureus and the human methicillin-resistant S. argenteus isolate MSHR1132T. Spectra obtained by MALDI-TOF MS and the analysis of available sequences in the genome databases identified several MALDI-TOF MS signals that clearly differentiate S. argenteus, the closely related Staphylococcus schweitzeri and S. aureus. In conclusion, in the absence of biochemical tests that identify the three species, mass spectrometry should be employed as method of choice.This work was supported by a grant of the Deutsches Zentrum für Infektionsforschung (DZIF) to Gabriele Bierbaum (TTU 08.902) and the Fundação para a Ciência e a Tecnologia (FCT) to Luís D.R. Melo (SFRH/BD/66166/2009) as well as by the BONFOR funds of the Medical Faculty of the University of Bonn and funds of the Centre International de Recherches Médicales de Franceville (CIRMF) and the Institut de Recherche pour le Développement (IRD).ElsevierUniversidade do MinhoSchuster, DominikRickmeyer, JasminGajdiss, MikeThye, ThorstenLorenzen, StephanReif, MarionJosten, MichaeleMelo, Luís D. R.Schmithausen, Ricarda M.Liégeois, FlorianSahl, Hans-GeorgGonzalez, Jean-Paul J.Nagel, MichaelBierbaum, Gabriele20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/44722engSchuster, Dominik; Rickmeyer, Jasmin; Gajdiss, Mike; Thye, Thorsten; Lorenzen, Stephan; Reif, Marion; Josten, Michaele; Melo, Luís D. R.; Schmithausen, Ricarda M.; Liégeois, Florian; Sahl, Hans-Georg; Gonzalez, Jean-Paul J.; Nagel, Michael; Bierbaum, Gabriele, Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape. International Journal of Medical Microbiology, 307(1), 57-63, 20171618-06071438-422110.1016/j.ijmm.2016.11.00327931949http://www.journals.elsevier.com/international-journal-of-medical-microbiology/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:42:14Zoai:repositorium.sdum.uminho.pt:1822/44722Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:39:26.171377Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape |
title |
Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape |
spellingShingle |
Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape Schuster, Dominik Staphylococcus argenteus Staphylococcus aureus Staphylococcus schweitzeri Gorilla gorilla ssp. gorilla MALDI-TOF MS Virulence determinants Science & Technology |
title_short |
Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape |
title_full |
Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape |
title_fullStr |
Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape |
title_full_unstemmed |
Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape |
title_sort |
Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape |
author |
Schuster, Dominik |
author_facet |
Schuster, Dominik Rickmeyer, Jasmin Gajdiss, Mike Thye, Thorsten Lorenzen, Stephan Reif, Marion Josten, Michaele Melo, Luís D. R. Schmithausen, Ricarda M. Liégeois, Florian Sahl, Hans-Georg Gonzalez, Jean-Paul J. Nagel, Michael Bierbaum, Gabriele |
author_role |
author |
author2 |
Rickmeyer, Jasmin Gajdiss, Mike Thye, Thorsten Lorenzen, Stephan Reif, Marion Josten, Michaele Melo, Luís D. R. Schmithausen, Ricarda M. Liégeois, Florian Sahl, Hans-Georg Gonzalez, Jean-Paul J. Nagel, Michael Bierbaum, Gabriele |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Schuster, Dominik Rickmeyer, Jasmin Gajdiss, Mike Thye, Thorsten Lorenzen, Stephan Reif, Marion Josten, Michaele Melo, Luís D. R. Schmithausen, Ricarda M. Liégeois, Florian Sahl, Hans-Georg Gonzalez, Jean-Paul J. Nagel, Michael Bierbaum, Gabriele |
dc.subject.por.fl_str_mv |
Staphylococcus argenteus Staphylococcus aureus Staphylococcus schweitzeri Gorilla gorilla ssp. gorilla MALDI-TOF MS Virulence determinants Science & Technology |
topic |
Staphylococcus argenteus Staphylococcus aureus Staphylococcus schweitzeri Gorilla gorilla ssp. gorilla MALDI-TOF MS Virulence determinants Science & Technology |
description |
The species Staphylococcus argenteus was separated recently from Staphylococcus aureus (Tong S.Y., F. Schaumburg, M.J. Ellington, J. Corander, B. Pichon, F. Leendertz, S.D. Bentley, J. Parkhill, D.C. Holt, G. Peters, and P.M. Giffard, 2015). The objective of this work was to characterise the genome of a non-human S. argenteus strain, which had been isolated from the faeces of a wild-living western lowland gorilla in Gabon, and analyse the spectrum of this species in matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). The full genome sequence revealed a scarcity of virulence genes and absence of resistance genes, indicating a decreased virulence potential compared to S. aureus and the human methicillin-resistant S. argenteus isolate MSHR1132T. Spectra obtained by MALDI-TOF MS and the analysis of available sequences in the genome databases identified several MALDI-TOF MS signals that clearly differentiate S. argenteus, the closely related Staphylococcus schweitzeri and S. aureus. In conclusion, in the absence of biochemical tests that identify the three species, mass spectrometry should be employed as method of choice. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/44722 |
url |
http://hdl.handle.net/1822/44722 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Schuster, Dominik; Rickmeyer, Jasmin; Gajdiss, Mike; Thye, Thorsten; Lorenzen, Stephan; Reif, Marion; Josten, Michaele; Melo, Luís D. R.; Schmithausen, Ricarda M.; Liégeois, Florian; Sahl, Hans-Georg; Gonzalez, Jean-Paul J.; Nagel, Michael; Bierbaum, Gabriele, Differentiation of Staphylococcus argenteus (formerly: Staphylococcus aureus clonal complex 75) by mass spectrometry from S. aureus using the first strain isolated from a wild African great ape. International Journal of Medical Microbiology, 307(1), 57-63, 2017 1618-0607 1438-4221 10.1016/j.ijmm.2016.11.003 27931949 http://www.journals.elsevier.com/international-journal-of-medical-microbiology/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132935732854784 |