Hybrid microfluidic platform for multifactorial analysis based on electrical impedance, refractometry, optical absorption and fluorescence
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | https://doi.org/10.3390/mi7100181 |
Resumo: | FEDER funds through the COMPETE 2020 Programme National Funds through Fundacao para a Ciencia e a Tecnologia (FCT) (UID/CTM/50025/2013, PTDC/SAU-BEB/102247/2008) project "Project" NanoSTIMA: Macro-to-Nano Human Sensing: Towards Integrated Multimodal Health Monitoring and Analytics - North Portugal Regional Operational Programme (NORTE-01-0145-FEDER-000016) European Regional Development Fund (ERDF) North Portugal Regional Operational Program under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF) (NORTE-07-0124-FEDER-000058) national funds, through the Portuguese funding agency, FCT |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Hybrid microfluidic platform for multifactorial analysis based on electrical impedance, refractometry, optical absorption and fluorescencesingle cell analysislabel-free methodsimpedance spectroscopyrefractometryoptical absorptionhybrid microfluidic chipFEDER funds through the COMPETE 2020 Programme National Funds through Fundacao para a Ciencia e a Tecnologia (FCT) (UID/CTM/50025/2013, PTDC/SAU-BEB/102247/2008) project "Project" NanoSTIMA: Macro-to-Nano Human Sensing: Towards Integrated Multimodal Health Monitoring and Analytics - North Portugal Regional Operational Programme (NORTE-01-0145-FEDER-000016) European Regional Development Fund (ERDF) North Portugal Regional Operational Program under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF) (NORTE-07-0124-FEDER-000058) national funds, through the Portuguese funding agency, FCTThis paper describes the development of a novel microfluidic platform for multifactorial analysis integrating four label-free detection methods: electrical impedance, refractometry, optical absorption and fluorescence. We present the rationale for the design and the details of the microfabrication of this multifactorial hybrid microfluidic chip. The structure of the platform consists of a three-dimensionally patterned polydimethylsiloxane top part attached to a bottom SU-8 epoxy-based negative photoresist part, where microelectrodes and optical fibers are incorporated to enable impedance and optical analysis. As a proof of concept, the chip functions have been tested and explored, enabling a diversity of applications: (i) impedance-based identification of the size of micro beads, as well as counting and distinguishing of erythrocytes by their volume or membrane properties; (ii) simultaneous determination of the refractive index and optical absorption properties of solutions; and (iii) fluorescence-based bead countingDCM - Departamento de Ciência dos MateriaisCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNPereira, F.M.Bernacka-Wojcik, IwonaRibeiro, R.S.R.Lobato, Maria TeresaFortunato, E.Martins, Rodrigo Ferrão de PaivaIgreja, R.Jorge, Pedro A SÁguas, H.Oliva, A.M.G.2017-06-04T00:05:27Z2016-10-072016-10-07T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.3390/mi7100181engPURE: 2596786https://www.scopus.com/inward/record.uri?eid=2-s2.0-84994806266&doi=10.3390%2fmi7100181&partnerID=40&md5=d1952ba57622eedbf79516637bb33e4dhttps://doi.org/10.3390/mi7100181info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:08:03Zoai:run.unl.pt:10362/21409Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:26:46.811172Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Hybrid microfluidic platform for multifactorial analysis based on electrical impedance, refractometry, optical absorption and fluorescence |
| title |
Hybrid microfluidic platform for multifactorial analysis based on electrical impedance, refractometry, optical absorption and fluorescence |
| spellingShingle |
Hybrid microfluidic platform for multifactorial analysis based on electrical impedance, refractometry, optical absorption and fluorescence Pereira, F.M. single cell analysis label-free methods impedance spectroscopy refractometry optical absorption hybrid microfluidic chip |
| title_short |
Hybrid microfluidic platform for multifactorial analysis based on electrical impedance, refractometry, optical absorption and fluorescence |
| title_full |
Hybrid microfluidic platform for multifactorial analysis based on electrical impedance, refractometry, optical absorption and fluorescence |
| title_fullStr |
Hybrid microfluidic platform for multifactorial analysis based on electrical impedance, refractometry, optical absorption and fluorescence |
| title_full_unstemmed |
Hybrid microfluidic platform for multifactorial analysis based on electrical impedance, refractometry, optical absorption and fluorescence |
| title_sort |
Hybrid microfluidic platform for multifactorial analysis based on electrical impedance, refractometry, optical absorption and fluorescence |
| author |
Pereira, F.M. |
| author_facet |
Pereira, F.M. Bernacka-Wojcik, Iwona Ribeiro, R.S.R. Lobato, Maria Teresa Fortunato, E. Martins, Rodrigo Ferrão de Paiva Igreja, R. Jorge, Pedro A S Águas, H. Oliva, A.M.G. |
| author_role |
author |
| author2 |
Bernacka-Wojcik, Iwona Ribeiro, R.S.R. Lobato, Maria Teresa Fortunato, E. Martins, Rodrigo Ferrão de Paiva Igreja, R. Jorge, Pedro A S Águas, H. Oliva, A.M.G. |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
DCM - Departamento de Ciência dos Materiais CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
| dc.contributor.author.fl_str_mv |
Pereira, F.M. Bernacka-Wojcik, Iwona Ribeiro, R.S.R. Lobato, Maria Teresa Fortunato, E. Martins, Rodrigo Ferrão de Paiva Igreja, R. Jorge, Pedro A S Águas, H. Oliva, A.M.G. |
| dc.subject.por.fl_str_mv |
single cell analysis label-free methods impedance spectroscopy refractometry optical absorption hybrid microfluidic chip |
| topic |
single cell analysis label-free methods impedance spectroscopy refractometry optical absorption hybrid microfluidic chip |
| description |
FEDER funds through the COMPETE 2020 Programme National Funds through Fundacao para a Ciencia e a Tecnologia (FCT) (UID/CTM/50025/2013, PTDC/SAU-BEB/102247/2008) project "Project" NanoSTIMA: Macro-to-Nano Human Sensing: Towards Integrated Multimodal Health Monitoring and Analytics - North Portugal Regional Operational Programme (NORTE-01-0145-FEDER-000016) European Regional Development Fund (ERDF) North Portugal Regional Operational Program under the National Strategic Reference Framework (NSRF), through the European Regional Development Fund (ERDF) (NORTE-07-0124-FEDER-000058) national funds, through the Portuguese funding agency, FCT |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016-10-07 2016-10-07T00:00:00Z 2017-06-04T00:05:27Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://doi.org/10.3390/mi7100181 |
| url |
https://doi.org/10.3390/mi7100181 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
PURE: 2596786 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84994806266&doi=10.3390%2fmi7100181&partnerID=40&md5=d1952ba57622eedbf79516637bb33e4d https://doi.org/10.3390/mi7100181 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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