A copper protein and a cytochrome bind at the same site on bacterial cytochrome c peroxidase
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/8711 |
Resumo: | Biochemistry, 2004, 43 (46), pp 14566–14576 DOI: 10.1021/bi0485833 |
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A copper protein and a cytochrome bind at the same site on bacterial cytochrome c peroxidaseBiochemistry, 2004, 43 (46), pp 14566–14576 DOI: 10.1021/bi0485833Pseudoazurin binds at a single site on cytochrome c peroxidase from Paracoccus pantotrophus with a K(d) of 16.4 microM at 25 degrees C, pH 6.0, in an endothermic reaction that is driven by a large entropy change. Sedimentation velocity experiments confirmed the presence of a single site, although results at higher pseudoazurin concentrations are complicated by the dimerization of the protein. Microcalorimetry, ultracentrifugation, and (1)H NMR spectroscopy studies in which cytochrome c550, pseudoazurin, and cytochrome c peroxidase were all present could be modeled using a competitive binding algorithm. Molecular docking simulation of the binding of pseudoazurin to the peroxidase in combination with the chemical shift perturbation pattern for pseudoazurin in the presence of the peroxidase revealed a group of solutions that were situated close to the electron-transferring heme with Cu-Fe distances of about 14 A. This is consistent with the results of (1)H NMR spectroscopy, which showed that pseudoazurin binds closely enough to the electron-transferring heme of the peroxidase to perturb its set of heme methyl resonances. We conclude that cytochrome c550 and pseudoazurin bind at the same site on the cytochrome c peroxidase and that the pair of electrons required to restore the enzyme to its active state after turnover are delivered one-by-one to the electron-transferring heme.American Chemical SocietyRUNPauleta, Sofia R.Cooper, AlanNutley, MargaretErrington, NeilGoodhew, Celia F.Moura, IsabelMoura, José J. G.2013-02-06T11:55:21Z20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/8711eng0006-2960info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:41:36Zoai:run.unl.pt:10362/8711Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:18:23.558901Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A copper protein and a cytochrome bind at the same site on bacterial cytochrome c peroxidase |
title |
A copper protein and a cytochrome bind at the same site on bacterial cytochrome c peroxidase |
spellingShingle |
A copper protein and a cytochrome bind at the same site on bacterial cytochrome c peroxidase Pauleta, Sofia R. |
title_short |
A copper protein and a cytochrome bind at the same site on bacterial cytochrome c peroxidase |
title_full |
A copper protein and a cytochrome bind at the same site on bacterial cytochrome c peroxidase |
title_fullStr |
A copper protein and a cytochrome bind at the same site on bacterial cytochrome c peroxidase |
title_full_unstemmed |
A copper protein and a cytochrome bind at the same site on bacterial cytochrome c peroxidase |
title_sort |
A copper protein and a cytochrome bind at the same site on bacterial cytochrome c peroxidase |
author |
Pauleta, Sofia R. |
author_facet |
Pauleta, Sofia R. Cooper, Alan Nutley, Margaret Errington, Neil Goodhew, Celia F. Moura, Isabel Moura, José J. G. |
author_role |
author |
author2 |
Cooper, Alan Nutley, Margaret Errington, Neil Goodhew, Celia F. Moura, Isabel Moura, José J. G. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
RUN |
dc.contributor.author.fl_str_mv |
Pauleta, Sofia R. Cooper, Alan Nutley, Margaret Errington, Neil Goodhew, Celia F. Moura, Isabel Moura, José J. G. |
description |
Biochemistry, 2004, 43 (46), pp 14566–14576 DOI: 10.1021/bi0485833 |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004 2004-01-01T00:00:00Z 2013-02-06T11:55:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/8711 |
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http://hdl.handle.net/10362/8711 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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0006-2960 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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