A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans

Detalhes bibliográficos
Autor(a) principal: McGinnity, Dermot F.
Data de Publicação: 1996
Outros Autores: Devreese, Bart, Prazeres, Susana, Van Beeumen, Jozef, Mourait, Isabel, Moura, José J. G., Pettigrew, Graham W.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1074/jbc.271.19.11126
Resumo: Wellcome Trust
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spelling A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificansBiochemistryMolecular BiologyCell BiologyWellcome TrustThe diheme cytochrome e peroxidase from Paracoccus denitrificans was modified with the histidine-specific reagent diethyl pyrocarbonate. At low excess of reagent, 1 mol of histidine was modified in the oxidized enzyme, and modification was associated with loss of the ability to form the active state. With time, the modification reversed, and the ability to form the active state was recovered. The agreement between the spectrophotometric measurement of histidine modification and radioactive incorporation using a radiolabeled reagent indicated little modification of other amino acids. However, the reversal of histidine modification observed spectrophotometrically was not matched by loss of radioactivity, and we propose a slow transfer of the ethoxyformyl group to an unidentified amino acid. The presence of CN- bound to the active peroxidatic site of the enzyme led to complete protection of the essential histidine from modification. Limited subtilisin treatment of the native enzyme followed by tryptic digest of the C-terminal fragment (residues 251-338) showed that radioactivity was located in a peptide containing a single histidine at position 275. We propose that this conserved residue, in a highly conserved region, is central to the function of the active mixed-valence state.DQ - Departamento de QuímicaRUNMcGinnity, Dermot F.Devreese, BartPrazeres, SusanaVan Beeumen, JozefMourait, IsabelMoura, José J. G.Pettigrew, Graham W.2019-09-09T22:37:13Z1996-06-041996-06-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttps://doi.org/10.1074/jbc.271.19.11126eng0021-9258PURE: 14624705http://www.scopus.com/inward/record.url?scp=17544370121&partnerID=8YFLogxKhttps://doi.org/10.1074/jbc.271.19.11126info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:35:37Zoai:run.unl.pt:10362/80627Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:35:53.040875Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans
title A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans
spellingShingle A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans
McGinnity, Dermot F.
Biochemistry
Molecular Biology
Cell Biology
title_short A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans
title_full A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans
title_fullStr A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans
title_full_unstemmed A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans
title_sort A single histidine is required for activity of cytochrome c peroxidase from Paracoccus denitrificans
author McGinnity, Dermot F.
author_facet McGinnity, Dermot F.
Devreese, Bart
Prazeres, Susana
Van Beeumen, Jozef
Mourait, Isabel
Moura, José J. G.
Pettigrew, Graham W.
author_role author
author2 Devreese, Bart
Prazeres, Susana
Van Beeumen, Jozef
Mourait, Isabel
Moura, José J. G.
Pettigrew, Graham W.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv McGinnity, Dermot F.
Devreese, Bart
Prazeres, Susana
Van Beeumen, Jozef
Mourait, Isabel
Moura, José J. G.
Pettigrew, Graham W.
dc.subject.por.fl_str_mv Biochemistry
Molecular Biology
Cell Biology
topic Biochemistry
Molecular Biology
Cell Biology
description Wellcome Trust
publishDate 1996
dc.date.none.fl_str_mv 1996-06-04
1996-06-04T00:00:00Z
2019-09-09T22:37:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1074/jbc.271.19.11126
url https://doi.org/10.1074/jbc.271.19.11126
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0021-9258
PURE: 14624705
http://www.scopus.com/inward/record.url?scp=17544370121&partnerID=8YFLogxK
https://doi.org/10.1074/jbc.271.19.11126
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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