Valorization of passion fruit stalk by the preparation of cellulose nanofibers and immobilization of trypsin
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/71168 |
Resumo: | Fibers from passion fruit stalks were first recovered with a pulping and bleaching process. Two mechanical treatments were further applied to the fibers, homogenization (with and without ultrasound), and blender application. The effect of those treatments on fibers was evaluated. The chemical composition of the different stages of fibers undergoing treatment were measured according to TAPPI standards and were also analyzed by SEM, FTIR, and XRD. Trypsin was immobilized by adsorption and by covalent binding. The biocatalyst support-trypsin was evaluated in terms of immobilization yield, retention, and enzymatic activity. The experimental results demonstrated that the final cellulose concentration in the fibers was 44 % higher than that in the raw stalks. The cellulose nanofibers obtained by homogenization presented a size distribution between 20200 nm, and the application of ultrasound did not show a significant effect on size (between 50 to 300 nm). Trypsin immobilized using glycidol presented an immobilization yield of 67 % and presented higher retention and enzymatic activity (1.17±0.05 U/mg protein and 44.0±2.0 %, respectively). These results show that passion fruit stalks can be successfully used as a source of cellulose nanofibers and also can be used as carriers for the immobilization of trypsin. |
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Valorization of passion fruit stalk by the preparation of cellulose nanofibers and immobilization of trypsinPassion fruitAgro-industrial wasteCellulose nanofiberImmobilizationTrypsinScience & TechnologyFibers from passion fruit stalks were first recovered with a pulping and bleaching process. Two mechanical treatments were further applied to the fibers, homogenization (with and without ultrasound), and blender application. The effect of those treatments on fibers was evaluated. The chemical composition of the different stages of fibers undergoing treatment were measured according to TAPPI standards and were also analyzed by SEM, FTIR, and XRD. Trypsin was immobilized by adsorption and by covalent binding. The biocatalyst support-trypsin was evaluated in terms of immobilization yield, retention, and enzymatic activity. The experimental results demonstrated that the final cellulose concentration in the fibers was 44 % higher than that in the raw stalks. The cellulose nanofibers obtained by homogenization presented a size distribution between 20200 nm, and the application of ultrasound did not show a significant effect on size (between 50 to 300 nm). Trypsin immobilized using glycidol presented an immobilization yield of 67 % and presented higher retention and enzymatic activity (1.17±0.05 U/mg protein and 44.0±2.0 %, respectively). These results show that passion fruit stalks can be successfully used as a source of cellulose nanofibers and also can be used as carriers for the immobilization of trypsin.The authors acknowledge the financial support of Universidad Nacional de Colombia (Hermes code project 34573), COLCIENCIAS call 727-2015 National Doctorates. FONTAGRO (ATN/RF-16111-RG Productividad y Competitividad Frutícola Andina), and Erasmus+Programme Key Action 1. This work was also supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of the UID/BIO/04469/2020 unit and by the BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersionSpringerUniversidade do MinhoRodríguez-Restrepo, Yeimy A.Rocha, Cristina M. R.Teixeira, J. A.Orrego, Carlos E.20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/71168engRodríguez-Restrepo, Yeimy A.; Rocha, Cristina M. R.; Teixeira, José A.; Orrego, Carlos E., Valorization of passion fruit stalk by the preparation of cellulose nanofibers and immobilization of trypsin. Fibers and Polymers, 21(12), 2807-2816, 20201229-919710.1007/s12221-020-1342-2http://www.springer.com/chemistry/polymer+science/journal/12221info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:43:23Zoai:repositorium.sdum.uminho.pt:1822/71168Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:40:51.932812Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Valorization of passion fruit stalk by the preparation of cellulose nanofibers and immobilization of trypsin |
title |
Valorization of passion fruit stalk by the preparation of cellulose nanofibers and immobilization of trypsin |
spellingShingle |
Valorization of passion fruit stalk by the preparation of cellulose nanofibers and immobilization of trypsin Rodríguez-Restrepo, Yeimy A. Passion fruit Agro-industrial waste Cellulose nanofiber Immobilization Trypsin Science & Technology |
title_short |
Valorization of passion fruit stalk by the preparation of cellulose nanofibers and immobilization of trypsin |
title_full |
Valorization of passion fruit stalk by the preparation of cellulose nanofibers and immobilization of trypsin |
title_fullStr |
Valorization of passion fruit stalk by the preparation of cellulose nanofibers and immobilization of trypsin |
title_full_unstemmed |
Valorization of passion fruit stalk by the preparation of cellulose nanofibers and immobilization of trypsin |
title_sort |
Valorization of passion fruit stalk by the preparation of cellulose nanofibers and immobilization of trypsin |
author |
Rodríguez-Restrepo, Yeimy A. |
author_facet |
Rodríguez-Restrepo, Yeimy A. Rocha, Cristina M. R. Teixeira, J. A. Orrego, Carlos E. |
author_role |
author |
author2 |
Rocha, Cristina M. R. Teixeira, J. A. Orrego, Carlos E. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Rodríguez-Restrepo, Yeimy A. Rocha, Cristina M. R. Teixeira, J. A. Orrego, Carlos E. |
dc.subject.por.fl_str_mv |
Passion fruit Agro-industrial waste Cellulose nanofiber Immobilization Trypsin Science & Technology |
topic |
Passion fruit Agro-industrial waste Cellulose nanofiber Immobilization Trypsin Science & Technology |
description |
Fibers from passion fruit stalks were first recovered with a pulping and bleaching process. Two mechanical treatments were further applied to the fibers, homogenization (with and without ultrasound), and blender application. The effect of those treatments on fibers was evaluated. The chemical composition of the different stages of fibers undergoing treatment were measured according to TAPPI standards and were also analyzed by SEM, FTIR, and XRD. Trypsin was immobilized by adsorption and by covalent binding. The biocatalyst support-trypsin was evaluated in terms of immobilization yield, retention, and enzymatic activity. The experimental results demonstrated that the final cellulose concentration in the fibers was 44 % higher than that in the raw stalks. The cellulose nanofibers obtained by homogenization presented a size distribution between 20200 nm, and the application of ultrasound did not show a significant effect on size (between 50 to 300 nm). Trypsin immobilized using glycidol presented an immobilization yield of 67 % and presented higher retention and enzymatic activity (1.17±0.05 U/mg protein and 44.0±2.0 %, respectively). These results show that passion fruit stalks can be successfully used as a source of cellulose nanofibers and also can be used as carriers for the immobilization of trypsin. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/71168 |
url |
http://hdl.handle.net/1822/71168 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Rodríguez-Restrepo, Yeimy A.; Rocha, Cristina M. R.; Teixeira, José A.; Orrego, Carlos E., Valorization of passion fruit stalk by the preparation of cellulose nanofibers and immobilization of trypsin. Fibers and Polymers, 21(12), 2807-2816, 2020 1229-9197 10.1007/s12221-020-1342-2 http://www.springer.com/chemistry/polymer+science/journal/12221 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132955412529152 |