Epigenetic marks in the mature pollen of Quercus suber L. (Fagaceae)

Detalhes bibliográficos
Autor(a) principal: Ribeiro, Teresa
Data de Publicação: 2009
Outros Autores: Viegas, Wanda, Morais-Cecilio, Leonor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/9040
Resumo: We have analysed the distribution of epigenetic marks for histone modifications at lysine residues H3 and H4, and DNA methylation, in the nuclei of mature pollen cells of the Angiosperm tree Quercus suber; a monoecious wind pollinated species with a protandrous system, and a long post-pollination period. The ultrasonic treatment developed for the isolation of pollen nuclei proved to be a fast and reliable method, preventing the interference of cell wall autofluorescence in the in situ immunolabelling assays. In contrast with previous studies on herbaceous species with short progamic phases, our results are consistent with a high level of silent (5-mC and H3K9me2) epigenetic marks on chromatin of the generative nucleus, and the prevalence of active marks (H3K9me3 and H4Kac) in the vegetative nucleus. The findings are discussed in terms of the pollination/fertilization timing strategy adopted by this plant species
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spelling Epigenetic marks in the mature pollen of Quercus suber L. (Fagaceae)epigenetic marksvegetative nucleusgenerative nucleusFagaceaedelayed fertilizationWe have analysed the distribution of epigenetic marks for histone modifications at lysine residues H3 and H4, and DNA methylation, in the nuclei of mature pollen cells of the Angiosperm tree Quercus suber; a monoecious wind pollinated species with a protandrous system, and a long post-pollination period. The ultrasonic treatment developed for the isolation of pollen nuclei proved to be a fast and reliable method, preventing the interference of cell wall autofluorescence in the in situ immunolabelling assays. In contrast with previous studies on herbaceous species with short progamic phases, our results are consistent with a high level of silent (5-mC and H3K9me2) epigenetic marks on chromatin of the generative nucleus, and the prevalence of active marks (H3K9me3 and H4Kac) in the vegetative nucleus. The findings are discussed in terms of the pollination/fertilization timing strategy adopted by this plant speciesSpringerRepositório da Universidade de LisboaRibeiro, TeresaViegas, WandaMorais-Cecilio, Leonor2015-07-22T13:07:57Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/9040eng"Sexual Plant Reproduction". ISSN 0934-0882. 22 (2009) 1-710.1007/s00497-008-0083-yinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-22T01:31:50Zoai:www.repository.utl.pt:10400.5/9040Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:55:53.188843Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Epigenetic marks in the mature pollen of Quercus suber L. (Fagaceae)
title Epigenetic marks in the mature pollen of Quercus suber L. (Fagaceae)
spellingShingle Epigenetic marks in the mature pollen of Quercus suber L. (Fagaceae)
Ribeiro, Teresa
epigenetic marks
vegetative nucleus
generative nucleus
Fagaceae
delayed fertilization
title_short Epigenetic marks in the mature pollen of Quercus suber L. (Fagaceae)
title_full Epigenetic marks in the mature pollen of Quercus suber L. (Fagaceae)
title_fullStr Epigenetic marks in the mature pollen of Quercus suber L. (Fagaceae)
title_full_unstemmed Epigenetic marks in the mature pollen of Quercus suber L. (Fagaceae)
title_sort Epigenetic marks in the mature pollen of Quercus suber L. (Fagaceae)
author Ribeiro, Teresa
author_facet Ribeiro, Teresa
Viegas, Wanda
Morais-Cecilio, Leonor
author_role author
author2 Viegas, Wanda
Morais-Cecilio, Leonor
author2_role author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Ribeiro, Teresa
Viegas, Wanda
Morais-Cecilio, Leonor
dc.subject.por.fl_str_mv epigenetic marks
vegetative nucleus
generative nucleus
Fagaceae
delayed fertilization
topic epigenetic marks
vegetative nucleus
generative nucleus
Fagaceae
delayed fertilization
description We have analysed the distribution of epigenetic marks for histone modifications at lysine residues H3 and H4, and DNA methylation, in the nuclei of mature pollen cells of the Angiosperm tree Quercus suber; a monoecious wind pollinated species with a protandrous system, and a long post-pollination period. The ultrasonic treatment developed for the isolation of pollen nuclei proved to be a fast and reliable method, preventing the interference of cell wall autofluorescence in the in situ immunolabelling assays. In contrast with previous studies on herbaceous species with short progamic phases, our results are consistent with a high level of silent (5-mC and H3K9me2) epigenetic marks on chromatin of the generative nucleus, and the prevalence of active marks (H3K9me3 and H4Kac) in the vegetative nucleus. The findings are discussed in terms of the pollination/fertilization timing strategy adopted by this plant species
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
2015-07-22T13:07:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/9040
url http://hdl.handle.net/10400.5/9040
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Sexual Plant Reproduction". ISSN 0934-0882. 22 (2009) 1-7
10.1007/s00497-008-0083-y
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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