Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies

Detalhes bibliográficos
Autor(a) principal: Balcão, Victor M.
Data de Publicação: 1998
Outros Autores: Oliveira, Teresa A., Malcata, F. Xavier
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/5958
Resumo: The present communication reports experimental and modelling work pertaining to the independent roles of pH and temperature on deactivation of a crude lipase from Mucor javanicus. Experimental data oflipolytic activities were generated by a classic pH-stat assay on a triolein emulsion following incubation at several pH values for a fixed time, or at several temperatures for various times; postulated models were then fitted by nonlinear fitting to such data. The pH-dependence data were best fit by assumption of three forms of enzyme with increasing states of protonation, with pKa values of 6.2 and 11.3, respectively, where only the intermediate form is stable within the time frame considered. The thermal-dependence data were best fit by assumption of parallel steps of deactivation and rearrangement, with activation energies of 228.8 and 221.7 kJ mol~l, respectively.
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spelling Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependenciesEnzymePHLipolytic activityMathematical modellingBatch reactorActivationThe present communication reports experimental and modelling work pertaining to the independent roles of pH and temperature on deactivation of a crude lipase from Mucor javanicus. Experimental data oflipolytic activities were generated by a classic pH-stat assay on a triolein emulsion following incubation at several pH values for a fixed time, or at several temperatures for various times; postulated models were then fitted by nonlinear fitting to such data. The pH-dependence data were best fit by assumption of three forms of enzyme with increasing states of protonation, with pKa values of 6.2 and 11.3, respectively, where only the intermediate form is stable within the time frame considered. The thermal-dependence data were best fit by assumption of parallel steps of deactivation and rearrangement, with activation energies of 228.8 and 221.7 kJ mol~l, respectively.Informa HealthcareVeritati - Repositório Institucional da Universidade Católica PortuguesaBalcão, Victor M.Oliveira, Teresa A.Malcata, F. Xavier2011-10-11T12:06:37Z19981998-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/5958engBALCÃO, Victor M; OLIVEIRA, Teresa A.; MALCATA, F. Xavier -Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies. Biocatalysis and Biotransformation. ISSN 1024-2422. Vol. 16 (1998), p. 45-66info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:10:58Zoai:repositorio.ucp.pt:10400.14/5958Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:06:05.780693Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies
title Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies
spellingShingle Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies
Balcão, Victor M.
Enzyme
PH
Lipolytic activity
Mathematical modelling
Batch reactor
Activation
title_short Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies
title_full Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies
title_fullStr Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies
title_full_unstemmed Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies
title_sort Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies
author Balcão, Victor M.
author_facet Balcão, Victor M.
Oliveira, Teresa A.
Malcata, F. Xavier
author_role author
author2 Oliveira, Teresa A.
Malcata, F. Xavier
author2_role author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Balcão, Victor M.
Oliveira, Teresa A.
Malcata, F. Xavier
dc.subject.por.fl_str_mv Enzyme
PH
Lipolytic activity
Mathematical modelling
Batch reactor
Activation
topic Enzyme
PH
Lipolytic activity
Mathematical modelling
Batch reactor
Activation
description The present communication reports experimental and modelling work pertaining to the independent roles of pH and temperature on deactivation of a crude lipase from Mucor javanicus. Experimental data oflipolytic activities were generated by a classic pH-stat assay on a triolein emulsion following incubation at several pH values for a fixed time, or at several temperatures for various times; postulated models were then fitted by nonlinear fitting to such data. The pH-dependence data were best fit by assumption of three forms of enzyme with increasing states of protonation, with pKa values of 6.2 and 11.3, respectively, where only the intermediate form is stable within the time frame considered. The thermal-dependence data were best fit by assumption of parallel steps of deactivation and rearrangement, with activation energies of 228.8 and 221.7 kJ mol~l, respectively.
publishDate 1998
dc.date.none.fl_str_mv 1998
1998-01-01T00:00:00Z
2011-10-11T12:06:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/5958
url http://hdl.handle.net/10400.14/5958
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv BALCÃO, Victor M; OLIVEIRA, Teresa A.; MALCATA, F. Xavier -Stability of a commercial lipase from Mucor javanicus: kinetic modeling of pH and temperature dependencies. Biocatalysis and Biotransformation. ISSN 1024-2422. Vol. 16 (1998), p. 45-66
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Informa Healthcare
publisher.none.fl_str_mv Informa Healthcare
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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