Induction of neurite outgrowth in 3D hydrogel-based environments
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/51421 |
Resumo: | The ability of peripheral nervous system (PNS) axons to regenerate and re-innervate their targets after an injury has been widely recognized. However, despite the considerable advances made in microsurgical techniques, complete functional recovery is rarely achieved, especially for severe peripheral nerve injuries (PNIs). Therefore, alternative therapies that can successfully repair peripheral nerves are still essential. In recent years the use of biodegradable hydrogels enriched with growth-supporting and guidance cues, cell transplantation, and biomolecular therapies have been explored for the treatment of PNIs. Bearing this in mind, the aim of this study was to assess whether Gly-Arg-Gly-Asp-Ser synthetic peptide (GRGDS)-modified gellan gum (GG) based hydrogels could foster an amenable environment for neurite/axonal growth. Additionally, strategies to further improve the rate of neurite outgrowth were also tested, namely the use of adipose tissue derived stem cells (ASCs), as well as the glial derived neurotrophic factor (GDNF). In order to increase its stability and enhance its bioactivity, the GDNF was conjugated covalently to iron oxide nanoparticles (IONPs). The impact of hydrogel modification as well as the effect of the GDNF-IONPs on ASC behavior was also screened. The results revealed that the GRGDS-GG hydrogel was able to support dorsal root ganglia (DRG)-based neurite outgrowth, which was not observed for non-modified hydrogels. Moreover, the modified hydrogels were also able to support ASCs attachment. In contrast, the presence of the GDNF-IONPs had no positive or negative impact on ASC behavior. Further experiments revealed that the presence of ASCs in the hydrogel improved axonal growth. On the other hand, GDNF-IONPs alone or combined with ASCs significantly increased neurite outgrowth from DRGs, suggesting a beneficial role of the proposed strategy for future applications in PNI regenerative medicine |
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Induction of neurite outgrowth in 3D hydrogel-based environmentsgellan-gum hydrogelsGRGDSadipose tissue-derived stem cellsglial-cell line derived neurotrophic factoriron oxide nanoparticlesperipheral nerve repairCiências Médicas::Biotecnologia MédicaScience & TechnologyThe ability of peripheral nervous system (PNS) axons to regenerate and re-innervate their targets after an injury has been widely recognized. However, despite the considerable advances made in microsurgical techniques, complete functional recovery is rarely achieved, especially for severe peripheral nerve injuries (PNIs). Therefore, alternative therapies that can successfully repair peripheral nerves are still essential. In recent years the use of biodegradable hydrogels enriched with growth-supporting and guidance cues, cell transplantation, and biomolecular therapies have been explored for the treatment of PNIs. Bearing this in mind, the aim of this study was to assess whether Gly-Arg-Gly-Asp-Ser synthetic peptide (GRGDS)-modified gellan gum (GG) based hydrogels could foster an amenable environment for neurite/axonal growth. Additionally, strategies to further improve the rate of neurite outgrowth were also tested, namely the use of adipose tissue derived stem cells (ASCs), as well as the glial derived neurotrophic factor (GDNF). In order to increase its stability and enhance its bioactivity, the GDNF was conjugated covalently to iron oxide nanoparticles (IONPs). The impact of hydrogel modification as well as the effect of the GDNF-IONPs on ASC behavior was also screened. The results revealed that the GRGDS-GG hydrogel was able to support dorsal root ganglia (DRG)-based neurite outgrowth, which was not observed for non-modified hydrogels. Moreover, the modified hydrogels were also able to support ASCs attachment. In contrast, the presence of the GDNF-IONPs had no positive or negative impact on ASC behavior. Further experiments revealed that the presence of ASCs in the hydrogel improved axonal growth. On the other hand, GDNF-IONPs alone or combined with ASCs significantly increased neurite outgrowth from DRGs, suggesting a beneficial role of the proposed strategy for future applications in PNI regenerative medicineEU-FP7-Health-2011-collaborative project 278612, Biohybrid—Templates for peripheral nerve regeneration; Prémios Santa Casa Neurociências—Prize Melo e Castro for Spinal Cord Injury Research; Portuguese Foundation for Science and Technology (IF Development Grant to A J Salgado; Post-Doctoral fellowship to N A Silva — SFRH/BPD/97701/2013); co-funded by Programa Operacional Regional do Norte (ON.2—O Novo Norte), ao abrigo do Quadro de Referência Estratégico Nacional (QREN), através do Fundo Europeu de Desenvolvimento Regional (FEDER); Professor Jeffrey Gimble at the Tulane University Center for Stem Cell Research and Regenerative Medicine and LaCell LLC (New Orleans, Louisiana, USA) for kindly providing the ASCs used in this studyinfo:eu-repo/semantics/publishedVersionIOP PublishingUniversidade do MinhoSilva, Rita Catarina Assunção RibeiroOliveira, Cátia Andreia CostaZiv-Polat, OfraGomes, Eduardo Domingos CorreiaSahar, AbrahamSousa, NunoSilva, Nuno A.Salgado, A. J.2015-10-202015-10-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/51421engAssunção-Silva, R. C., Oliveira, C. C., Ziv-Polat, O., et. al. (2015). Induction of neurite outgrowth in 3D hydrogel-based environments. Biomedical Materials, 10(5), 0510011748-604110.1088/1748-6041/10/5/05100126480959http://iopscience.iop.org/article/10.1088/1748-6041/10/5/051001/metainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:06:21Zoai:repositorium.sdum.uminho.pt:1822/51421Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:56:59.979404Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Induction of neurite outgrowth in 3D hydrogel-based environments |
title |
Induction of neurite outgrowth in 3D hydrogel-based environments |
spellingShingle |
Induction of neurite outgrowth in 3D hydrogel-based environments Silva, Rita Catarina Assunção Ribeiro gellan-gum hydrogels GRGDS adipose tissue-derived stem cells glial-cell line derived neurotrophic factor iron oxide nanoparticles peripheral nerve repair Ciências Médicas::Biotecnologia Médica Science & Technology |
title_short |
Induction of neurite outgrowth in 3D hydrogel-based environments |
title_full |
Induction of neurite outgrowth in 3D hydrogel-based environments |
title_fullStr |
Induction of neurite outgrowth in 3D hydrogel-based environments |
title_full_unstemmed |
Induction of neurite outgrowth in 3D hydrogel-based environments |
title_sort |
Induction of neurite outgrowth in 3D hydrogel-based environments |
author |
Silva, Rita Catarina Assunção Ribeiro |
author_facet |
Silva, Rita Catarina Assunção Ribeiro Oliveira, Cátia Andreia Costa Ziv-Polat, Ofra Gomes, Eduardo Domingos Correia Sahar, Abraham Sousa, Nuno Silva, Nuno A. Salgado, A. J. |
author_role |
author |
author2 |
Oliveira, Cátia Andreia Costa Ziv-Polat, Ofra Gomes, Eduardo Domingos Correia Sahar, Abraham Sousa, Nuno Silva, Nuno A. Salgado, A. J. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva, Rita Catarina Assunção Ribeiro Oliveira, Cátia Andreia Costa Ziv-Polat, Ofra Gomes, Eduardo Domingos Correia Sahar, Abraham Sousa, Nuno Silva, Nuno A. Salgado, A. J. |
dc.subject.por.fl_str_mv |
gellan-gum hydrogels GRGDS adipose tissue-derived stem cells glial-cell line derived neurotrophic factor iron oxide nanoparticles peripheral nerve repair Ciências Médicas::Biotecnologia Médica Science & Technology |
topic |
gellan-gum hydrogels GRGDS adipose tissue-derived stem cells glial-cell line derived neurotrophic factor iron oxide nanoparticles peripheral nerve repair Ciências Médicas::Biotecnologia Médica Science & Technology |
description |
The ability of peripheral nervous system (PNS) axons to regenerate and re-innervate their targets after an injury has been widely recognized. However, despite the considerable advances made in microsurgical techniques, complete functional recovery is rarely achieved, especially for severe peripheral nerve injuries (PNIs). Therefore, alternative therapies that can successfully repair peripheral nerves are still essential. In recent years the use of biodegradable hydrogels enriched with growth-supporting and guidance cues, cell transplantation, and biomolecular therapies have been explored for the treatment of PNIs. Bearing this in mind, the aim of this study was to assess whether Gly-Arg-Gly-Asp-Ser synthetic peptide (GRGDS)-modified gellan gum (GG) based hydrogels could foster an amenable environment for neurite/axonal growth. Additionally, strategies to further improve the rate of neurite outgrowth were also tested, namely the use of adipose tissue derived stem cells (ASCs), as well as the glial derived neurotrophic factor (GDNF). In order to increase its stability and enhance its bioactivity, the GDNF was conjugated covalently to iron oxide nanoparticles (IONPs). The impact of hydrogel modification as well as the effect of the GDNF-IONPs on ASC behavior was also screened. The results revealed that the GRGDS-GG hydrogel was able to support dorsal root ganglia (DRG)-based neurite outgrowth, which was not observed for non-modified hydrogels. Moreover, the modified hydrogels were also able to support ASCs attachment. In contrast, the presence of the GDNF-IONPs had no positive or negative impact on ASC behavior. Further experiments revealed that the presence of ASCs in the hydrogel improved axonal growth. On the other hand, GDNF-IONPs alone or combined with ASCs significantly increased neurite outgrowth from DRGs, suggesting a beneficial role of the proposed strategy for future applications in PNI regenerative medicine |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-20 2015-10-20T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/51421 |
url |
http://hdl.handle.net/1822/51421 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Assunção-Silva, R. C., Oliveira, C. C., Ziv-Polat, O., et. al. (2015). Induction of neurite outgrowth in 3D hydrogel-based environments. Biomedical Materials, 10(5), 051001 1748-6041 10.1088/1748-6041/10/5/051001 26480959 http://iopscience.iop.org/article/10.1088/1748-6041/10/5/051001/meta |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
IOP Publishing |
publisher.none.fl_str_mv |
IOP Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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