Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells

Detalhes bibliográficos
Autor(a) principal: Dong, Lan-Feng
Data de Publicação: 2017
Outros Autores: Kovarova, Jaromira, Bajzikova, Martina, Bezawork-Geleta, Ayenachew, Svec, David, Endaya, Berwini, Sachaphibulkij, Karishma, Coelho, Ana R., Sebkova, Natasa, Ruzickova, Anna, Tan, An S, Kluckova, Katarina, Judasova, Kristyna, Zamecnikova, Katerina, Rychtarcikova, Zuzana, Gopalan, Vinod, Andera, Ladislav, Sobol, Margarita, Yan, Bing, Pattnaik, Bijay, Bhatraju, Naveen, Truksa, Jaroslav, Stopka, Pavel, Hozak, Pavel, Lam, Alfred K., Sedlacek, Radislav, Oliveira, Paulo J., Kubista, Mikael, Agrawal, Anurag, Dvorakova-Hortova, Katerina, Rohlena, Jakub, Berridge, Michael V., Neuzil, Jiri
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/108138
https://doi.org/10.7554/eLife.22187
Resumo: Recently, we showed that generation of tumours in syngeneic mice by cells devoid of mitochondrial (mt) DNA (ρ0 cells) is linked to the acquisition of the host mtDNA. However, the mechanism of mtDNA movement between cells remains unresolved. To determine whether the transfer of mtDNA involves whole mitochondria, we injected B16ρ0 mouse melanoma cells into syngeneic C57BL/6Nsu9-DsRed2 mice that express red fluorescent protein in their mitochondria. We document that mtDNA is acquired by transfer of whole mitochondria from the host animal, leading to normalisation of mitochondrial respiration. Additionally, knockdown of key mitochondrial complex I (NDUFV1) and complex II (SDHC) subunits by shRNA in B16ρ0 cells abolished or significantly retarded their ability to form tumours. Collectively, these results show that intact mitochondria with their mtDNA payload are transferred in the developing tumour, and provide functional evidence for an essential role of oxidative phosphorylation in cancer.
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spelling Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cellscancer biology; mitochondrial transfer; mouse; respiration recovery; tumour growthAnimalsCell Line, TumorCell RespirationDNA, MitochondrialDisease Models, AnimalMelanomaMice, Inbred C57BLGene Transfer, HorizontalRecently, we showed that generation of tumours in syngeneic mice by cells devoid of mitochondrial (mt) DNA (ρ0 cells) is linked to the acquisition of the host mtDNA. However, the mechanism of mtDNA movement between cells remains unresolved. To determine whether the transfer of mtDNA involves whole mitochondria, we injected B16ρ0 mouse melanoma cells into syngeneic C57BL/6Nsu9-DsRed2 mice that express red fluorescent protein in their mitochondria. We document that mtDNA is acquired by transfer of whole mitochondria from the host animal, leading to normalisation of mitochondrial respiration. Additionally, knockdown of key mitochondrial complex I (NDUFV1) and complex II (SDHC) subunits by shRNA in B16ρ0 cells abolished or significantly retarded their ability to form tumours. Collectively, these results show that intact mitochondria with their mtDNA payload are transferred in the developing tumour, and provide functional evidence for an essential role of oxidative phosphorylation in cancer.eLife Sciences Publications2017-02-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/108138http://hdl.handle.net/10316/108138https://doi.org/10.7554/eLife.22187eng2050-084XDong, Lan-FengKovarova, JaromiraBajzikova, MartinaBezawork-Geleta, AyenachewSvec, DavidEndaya, BerwiniSachaphibulkij, KarishmaCoelho, Ana R.Sebkova, NatasaRuzickova, AnnaTan, An SKluckova, KatarinaJudasova, KristynaZamecnikova, KaterinaRychtarcikova, ZuzanaGopalan, VinodAndera, LadislavSobol, MargaritaYan, BingPattnaik, BijayBhatraju, NaveenTruksa, JaroslavStopka, PavelHozak, PavelLam, Alfred K.Sedlacek, RadislavOliveira, Paulo J.Kubista, MikaelAgrawal, AnuragDvorakova-Hortova, KaterinaRohlena, JakubBerridge, Michael V.Neuzil, Jiriinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-12T18:24:57Zoai:estudogeral.uc.pt:10316/108138Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:24:26.030118Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells
title Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells
spellingShingle Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells
Dong, Lan-Feng
cancer biology; mitochondrial transfer; mouse; respiration recovery; tumour growth
Animals
Cell Line, Tumor
Cell Respiration
DNA, Mitochondrial
Disease Models, Animal
Melanoma
Mice, Inbred C57BL
Gene Transfer, Horizontal
title_short Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells
title_full Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells
title_fullStr Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells
title_full_unstemmed Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells
title_sort Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells
author Dong, Lan-Feng
author_facet Dong, Lan-Feng
Kovarova, Jaromira
Bajzikova, Martina
Bezawork-Geleta, Ayenachew
Svec, David
Endaya, Berwini
Sachaphibulkij, Karishma
Coelho, Ana R.
Sebkova, Natasa
Ruzickova, Anna
Tan, An S
Kluckova, Katarina
Judasova, Kristyna
Zamecnikova, Katerina
Rychtarcikova, Zuzana
Gopalan, Vinod
Andera, Ladislav
Sobol, Margarita
Yan, Bing
Pattnaik, Bijay
Bhatraju, Naveen
Truksa, Jaroslav
Stopka, Pavel
Hozak, Pavel
Lam, Alfred K.
Sedlacek, Radislav
Oliveira, Paulo J.
Kubista, Mikael
Agrawal, Anurag
Dvorakova-Hortova, Katerina
Rohlena, Jakub
Berridge, Michael V.
Neuzil, Jiri
author_role author
author2 Kovarova, Jaromira
Bajzikova, Martina
Bezawork-Geleta, Ayenachew
Svec, David
Endaya, Berwini
Sachaphibulkij, Karishma
Coelho, Ana R.
Sebkova, Natasa
Ruzickova, Anna
Tan, An S
Kluckova, Katarina
Judasova, Kristyna
Zamecnikova, Katerina
Rychtarcikova, Zuzana
Gopalan, Vinod
Andera, Ladislav
Sobol, Margarita
Yan, Bing
Pattnaik, Bijay
Bhatraju, Naveen
Truksa, Jaroslav
Stopka, Pavel
Hozak, Pavel
Lam, Alfred K.
Sedlacek, Radislav
Oliveira, Paulo J.
Kubista, Mikael
Agrawal, Anurag
Dvorakova-Hortova, Katerina
Rohlena, Jakub
Berridge, Michael V.
Neuzil, Jiri
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Dong, Lan-Feng
Kovarova, Jaromira
Bajzikova, Martina
Bezawork-Geleta, Ayenachew
Svec, David
Endaya, Berwini
Sachaphibulkij, Karishma
Coelho, Ana R.
Sebkova, Natasa
Ruzickova, Anna
Tan, An S
Kluckova, Katarina
Judasova, Kristyna
Zamecnikova, Katerina
Rychtarcikova, Zuzana
Gopalan, Vinod
Andera, Ladislav
Sobol, Margarita
Yan, Bing
Pattnaik, Bijay
Bhatraju, Naveen
Truksa, Jaroslav
Stopka, Pavel
Hozak, Pavel
Lam, Alfred K.
Sedlacek, Radislav
Oliveira, Paulo J.
Kubista, Mikael
Agrawal, Anurag
Dvorakova-Hortova, Katerina
Rohlena, Jakub
Berridge, Michael V.
Neuzil, Jiri
dc.subject.por.fl_str_mv cancer biology; mitochondrial transfer; mouse; respiration recovery; tumour growth
Animals
Cell Line, Tumor
Cell Respiration
DNA, Mitochondrial
Disease Models, Animal
Melanoma
Mice, Inbred C57BL
Gene Transfer, Horizontal
topic cancer biology; mitochondrial transfer; mouse; respiration recovery; tumour growth
Animals
Cell Line, Tumor
Cell Respiration
DNA, Mitochondrial
Disease Models, Animal
Melanoma
Mice, Inbred C57BL
Gene Transfer, Horizontal
description Recently, we showed that generation of tumours in syngeneic mice by cells devoid of mitochondrial (mt) DNA (ρ0 cells) is linked to the acquisition of the host mtDNA. However, the mechanism of mtDNA movement between cells remains unresolved. To determine whether the transfer of mtDNA involves whole mitochondria, we injected B16ρ0 mouse melanoma cells into syngeneic C57BL/6Nsu9-DsRed2 mice that express red fluorescent protein in their mitochondria. We document that mtDNA is acquired by transfer of whole mitochondria from the host animal, leading to normalisation of mitochondrial respiration. Additionally, knockdown of key mitochondrial complex I (NDUFV1) and complex II (SDHC) subunits by shRNA in B16ρ0 cells abolished or significantly retarded their ability to form tumours. Collectively, these results show that intact mitochondria with their mtDNA payload are transferred in the developing tumour, and provide functional evidence for an essential role of oxidative phosphorylation in cancer.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/108138
http://hdl.handle.net/10316/108138
https://doi.org/10.7554/eLife.22187
url http://hdl.handle.net/10316/108138
https://doi.org/10.7554/eLife.22187
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2050-084X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv eLife Sciences Publications
publisher.none.fl_str_mv eLife Sciences Publications
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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