Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells

• Autor(a) principal: Dong, Lan-Feng
• Data de Publicação: 2017
• Outros Autores: Kovarova, Jaromira, Bajzikova, Martina, Bezawork-Geleta, Ayenachew, Svec, David, Endaya, Berwini, Sachaphibulkij, Karishma, Coelho, Ana R., Sebkova, Natasa, Ruzickova, Anna, Tan, An S, Kluckova, Katarina, Judasova, Kristyna, Zamecnikova, Katerina, Rychtarcikova, Zuzana, Gopalan, Vinod, Andera, Ladislav, Sobol, Margarita, Yan, Bing, Pattnaik, Bijay, Bhatraju, Naveen, Truksa, Jaroslav, Stopka, Pavel, Hozak, Pavel, Lam, Alfred K., Sedlacek, Radislav, Oliveira, Paulo J., Kubista, Mikael, Agrawal, Anurag, Dvorakova-Hortova, Katerina, Rohlena, Jakub, Berridge, Michael V., Neuzil, Jiri
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10316/108138; https://doi.org/10.7554/eLife.22187
• topic: cancer biology; mitochondrial transfer; mouse; respiration recovery; tumour growth; Animals; Cell Line, Tumor; Cell Respiration; DNA, Mitochondrial; Disease Models, Animal; Melanoma; Mice, Inbred C57BL; Gene Transfer, Horizontal

Recently, we showed that generation of tumours in syngeneic mice by cells devoid of mitochondrial (mt) DNA (ρ0 cells) is linked to the acquisition of the host mtDNA. However, the mechanism of mtDNA movement between cells remains unresolved. To determine whether the transfer of mtDNA involves whole mitochondria, we injected B16ρ0 mouse melanoma cells into syngeneic C57BL/6Nsu9-DsRed2 mice that express red fluorescent protein in their mitochondria. We document that mtDNA is acquired by transfer of whole mitochondria from the host animal, leading to normalisation of mitochondrial respiration. Additionally, knockdown of key mitochondrial complex I (NDUFV1) and complex II (SDHC) subunits by shRNA in B16ρ0 cells abolished or significantly retarded their ability to form tumours. Collectively, these results show that intact mitochondria with their mtDNA payload are transferred in the developing tumour, and provide functional evidence for an essential role of oxidative phosphorylation in cancer.