Catechol inhibits FADH2-linked respiration in rat liver mitochondrial fraction

Detalhes bibliográficos
Autor(a) principal: Barreto, George Emílio Sampaio
Data de Publicação: 2005
Outros Autores: Santos, Gleide Souza dos, Egito, Eryvaldo Sócrates Tabosa, El-Bachá, Ramon dos Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6573
Resumo: p.40-45
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spelling Barreto, George Emílio SampaioSantos, Gleide Souza dosEgito, Eryvaldo Sócrates TabosaEl-Bachá, Ramon dos SantosBarreto, George Emílio SampaioSantos, Gleide Souza dosEgito, Eryvaldo Sócrates TabosaEl-Bachá, Ramon dos Santos2012-08-09T14:25:04Z2012-08-09T14:25:04Z20051678-2674http://www.repositorio.ufba.br/ri/handle/ri/6573v. 20, n. 1p.40-45PURPOSE: The aim of this work was to investigate the hypothesis that catechol inhibits FADH2-linked basal respiration in mitochondria isolated from rat liver homogenates. Moreover, catechol ability to induce peroxidation of biomolecules in liver nuclear fractions was also studied. METHODS: Rat liver homogenates were incubated with 1mM 1,2-dihydroxybenzene (catechol) at pH 7.4 for up to 30 minutes. After that, mitochondrial fractions were isolated by differential centrifugation. Basal oxygen uptake was measured using a Clark-type electrode after the addition of 10 mM sodium succinate. Nuclear fractions were incubated in the presence of 1 mM catechol for 17 hours at room temperature and the peroxidation of biomolecules was investigated by the reaction with thiobarbituric acid, which was determined spectrophotometrically at 535 nm. RESULTS: Catechol induced a time-dependent partial inhibition of FADH2-linked basal mitochondrial respiration, however this substance was unable to induce a direct peroxidation of biomolecules in hepatic nuclear fractions. CONCLUSION: Catechol produced an inhibition of basal respiration associated to FADH2 in isolated liver mitochondria that could lead to cytotoxicity, ROS generation and cell death.Submitted by Texeira Ana (atanateixeira@gmail.com) on 2012-08-09T14:25:04Z No. of bitstreams: 1 Barreto, George Emílio Sampaio.pdf: 587216 bytes, checksum: e3f76e2adacedf8903cacccd6b6d7077 (MD5)Made available in DSpace on 2012-08-09T14:25:04Z (GMT). No. of bitstreams: 1 Barreto, George Emílio Sampaio.pdf: 587216 bytes, checksum: e3f76e2adacedf8903cacccd6b6d7077 (MD5) Previous issue date: 2005São PauloActa Cirúrgica Brasileira/SOBRADPEChttp://dx.doi.org/10.1590/S0102-86502005000700008reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBACatecholMitochondrial respirationPeroxidationCatechol inhibits FADH2-linked respiration in rat liver mitochondrial fractionActa Cirurgica Brasileirainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINALBarreto, George Emílio Sampaio.pdfBarreto, George Emílio Sampaio.pdfapplication/pdf587216https://repositorio.ufba.br/bitstream/ri/6573/1/Barreto%2c%20George%20Em%c3%adlio%20Sampaio.pdfe3f76e2adacedf8903cacccd6b6d7077MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/6573/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTBarreto, George Emílio Sampaio.pdf.txtBarreto, George Emílio Sampaio.pdf.txtExtracted texttext/plain23062https://repositorio.ufba.br/bitstream/ri/6573/3/Barreto%2c%20George%20Em%c3%adlio%20Sampaio.pdf.txte5512dd1c1b542ac32c11dd4135f790eMD53ri/65732022-07-05 14:03:23.118oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:23Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Catechol inhibits FADH2-linked respiration in rat liver mitochondrial fraction
dc.title.alternative.pt_BR.fl_str_mv Acta Cirurgica Brasileira
title Catechol inhibits FADH2-linked respiration in rat liver mitochondrial fraction
spellingShingle Catechol inhibits FADH2-linked respiration in rat liver mitochondrial fraction
Barreto, George Emílio Sampaio
Catechol
Mitochondrial respiration
Peroxidation
title_short Catechol inhibits FADH2-linked respiration in rat liver mitochondrial fraction
title_full Catechol inhibits FADH2-linked respiration in rat liver mitochondrial fraction
title_fullStr Catechol inhibits FADH2-linked respiration in rat liver mitochondrial fraction
title_full_unstemmed Catechol inhibits FADH2-linked respiration in rat liver mitochondrial fraction
title_sort Catechol inhibits FADH2-linked respiration in rat liver mitochondrial fraction
author Barreto, George Emílio Sampaio
author_facet Barreto, George Emílio Sampaio
Santos, Gleide Souza dos
Egito, Eryvaldo Sócrates Tabosa
El-Bachá, Ramon dos Santos
author_role author
author2 Santos, Gleide Souza dos
Egito, Eryvaldo Sócrates Tabosa
El-Bachá, Ramon dos Santos
author2_role author
author
author
dc.contributor.author.fl_str_mv Barreto, George Emílio Sampaio
Santos, Gleide Souza dos
Egito, Eryvaldo Sócrates Tabosa
El-Bachá, Ramon dos Santos
Barreto, George Emílio Sampaio
Santos, Gleide Souza dos
Egito, Eryvaldo Sócrates Tabosa
El-Bachá, Ramon dos Santos
dc.subject.por.fl_str_mv Catechol
Mitochondrial respiration
Peroxidation
topic Catechol
Mitochondrial respiration
Peroxidation
description p.40-45
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2012-08-09T14:25:04Z
dc.date.available.fl_str_mv 2012-08-09T14:25:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6573
dc.identifier.issn.none.fl_str_mv 1678-2674
dc.identifier.number.pt_BR.fl_str_mv v. 20, n. 1
identifier_str_mv 1678-2674
v. 20, n. 1
url http://www.repositorio.ufba.br/ri/handle/ri/6573
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Acta Cirúrgica Brasileira/SOBRADPEC
publisher.none.fl_str_mv Acta Cirúrgica Brasileira/SOBRADPEC
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1590/S0102-86502005000700008
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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