Immune status and hepatic antioxidant capacity of Gilthead Seabream Sparus aurata juveniles fed yeast and microalga derived β-glucans
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/17493 |
Resumo: | This work aimed to evaluate the effects of dietary supplementation with β-glucans extracted from yeast (Saccharomyces cerevisiae) and microalga (Phaeodactylum tricornutum) on gene expression, oxidative stress biomarkers and plasma immune parameters in gilthead seabream (Sparus aurata) juveniles. A practical commercial diet was used as the control (CTRL), and three others based on CTRL were further supplemented with different β-glucan extracts. One was derived from S. cerevisiae (diet MG) and two different extracts of 21% and 37% P. tricornutum-derived β-glucans (defined as Phaeo21 and Phaeo37), to give a final 0.06% β-glucan dietary concentration. Quadruplicate groups of 95 gilthead seabream (initial body weight: 4.1 ± 0.1 g) were fed to satiation three times a day for 8 weeks in a pulse-feeding regimen, with experimental diets intercalated with the CTRL dietary treatment every 2 weeks. After 8 weeks of feeding, all groups showed equal growth performance and no changes were found in plasma innate immune status. Nonetheless, fish groups fed β-glucans supplemented diets showed an improved anti-oxidant status compared to those fed CTRL at both sampling points (i.e., 2 and 8 weeks). The intestinal gene expression analysis highlighted the immunomodulatory role of Phaeo37 diet after 8 weeks, inducing an immune tolerance effect in gilthead seabream intestine, and a general down-regulation of immune-related gene expression. In conclusion, the results suggest that the dietary pulse administration of a P. tricornutum 37% enriched-β-glucans extract might be used as a counter-measure in a context of gut inflammation, due to its immune-tolerant and anti-oxidative effects. |
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Immune status and hepatic antioxidant capacity of Gilthead Seabream Sparus aurata juveniles fed yeast and microalga derived β-glucansStatus imunológico e capacidade antioxidante hepática de Gilthead Seabream Sparus aurata juveniles fed leveduras e microalga derivadas de Beta-glucanosSaccharomyces cerevisiaePhaeodactylum tricornutumSparus aurataβ-glucans;Pulse feedingImmune toleranceThis work aimed to evaluate the effects of dietary supplementation with β-glucans extracted from yeast (Saccharomyces cerevisiae) and microalga (Phaeodactylum tricornutum) on gene expression, oxidative stress biomarkers and plasma immune parameters in gilthead seabream (Sparus aurata) juveniles. A practical commercial diet was used as the control (CTRL), and three others based on CTRL were further supplemented with different β-glucan extracts. One was derived from S. cerevisiae (diet MG) and two different extracts of 21% and 37% P. tricornutum-derived β-glucans (defined as Phaeo21 and Phaeo37), to give a final 0.06% β-glucan dietary concentration. Quadruplicate groups of 95 gilthead seabream (initial body weight: 4.1 ± 0.1 g) were fed to satiation three times a day for 8 weeks in a pulse-feeding regimen, with experimental diets intercalated with the CTRL dietary treatment every 2 weeks. After 8 weeks of feeding, all groups showed equal growth performance and no changes were found in plasma innate immune status. Nonetheless, fish groups fed β-glucans supplemented diets showed an improved anti-oxidant status compared to those fed CTRL at both sampling points (i.e., 2 and 8 weeks). The intestinal gene expression analysis highlighted the immunomodulatory role of Phaeo37 diet after 8 weeks, inducing an immune tolerance effect in gilthead seabream intestine, and a general down-regulation of immune-related gene expression. In conclusion, the results suggest that the dietary pulse administration of a P. tricornutum 37% enriched-β-glucans extract might be used as a counter-measure in a context of gut inflammation, due to its immune-tolerant and anti-oxidative effects.NORTE-01-0145-FEDER-000040MDPISapientiaReis, BrunoGonçalves, Ana TeresaSantos, PauloSardinha, ManuelConceição, Luís E. C.Serradeiro, RenataPérez-Sánchez, JaumeCalduch-Giner, JosepSchmid-Staiger, UlrikeFrick, KonstantinDias, JorgeCostas, Benjamín2022-01-18T14:38:40Z2021-122021-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/17493eng1660-339710.3390/md19120653info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-13T02:05:54Zoai:sapientia.ualg.pt:10400.1/17493Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:07:27.988828Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Immune status and hepatic antioxidant capacity of Gilthead Seabream Sparus aurata juveniles fed yeast and microalga derived β-glucans Status imunológico e capacidade antioxidante hepática de Gilthead Seabream Sparus aurata juveniles fed leveduras e microalga derivadas de Beta-glucanos |
title |
Immune status and hepatic antioxidant capacity of Gilthead Seabream Sparus aurata juveniles fed yeast and microalga derived β-glucans |
spellingShingle |
Immune status and hepatic antioxidant capacity of Gilthead Seabream Sparus aurata juveniles fed yeast and microalga derived β-glucans Reis, Bruno Saccharomyces cerevisiae Phaeodactylum tricornutum Sparus aurata β-glucans; Pulse feeding Immune tolerance |
title_short |
Immune status and hepatic antioxidant capacity of Gilthead Seabream Sparus aurata juveniles fed yeast and microalga derived β-glucans |
title_full |
Immune status and hepatic antioxidant capacity of Gilthead Seabream Sparus aurata juveniles fed yeast and microalga derived β-glucans |
title_fullStr |
Immune status and hepatic antioxidant capacity of Gilthead Seabream Sparus aurata juveniles fed yeast and microalga derived β-glucans |
title_full_unstemmed |
Immune status and hepatic antioxidant capacity of Gilthead Seabream Sparus aurata juveniles fed yeast and microalga derived β-glucans |
title_sort |
Immune status and hepatic antioxidant capacity of Gilthead Seabream Sparus aurata juveniles fed yeast and microalga derived β-glucans |
author |
Reis, Bruno |
author_facet |
Reis, Bruno Gonçalves, Ana Teresa Santos, Paulo Sardinha, Manuel Conceição, Luís E. C. Serradeiro, Renata Pérez-Sánchez, Jaume Calduch-Giner, Josep Schmid-Staiger, Ulrike Frick, Konstantin Dias, Jorge Costas, Benjamín |
author_role |
author |
author2 |
Gonçalves, Ana Teresa Santos, Paulo Sardinha, Manuel Conceição, Luís E. C. Serradeiro, Renata Pérez-Sánchez, Jaume Calduch-Giner, Josep Schmid-Staiger, Ulrike Frick, Konstantin Dias, Jorge Costas, Benjamín |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Reis, Bruno Gonçalves, Ana Teresa Santos, Paulo Sardinha, Manuel Conceição, Luís E. C. Serradeiro, Renata Pérez-Sánchez, Jaume Calduch-Giner, Josep Schmid-Staiger, Ulrike Frick, Konstantin Dias, Jorge Costas, Benjamín |
dc.subject.por.fl_str_mv |
Saccharomyces cerevisiae Phaeodactylum tricornutum Sparus aurata β-glucans; Pulse feeding Immune tolerance |
topic |
Saccharomyces cerevisiae Phaeodactylum tricornutum Sparus aurata β-glucans; Pulse feeding Immune tolerance |
description |
This work aimed to evaluate the effects of dietary supplementation with β-glucans extracted from yeast (Saccharomyces cerevisiae) and microalga (Phaeodactylum tricornutum) on gene expression, oxidative stress biomarkers and plasma immune parameters in gilthead seabream (Sparus aurata) juveniles. A practical commercial diet was used as the control (CTRL), and three others based on CTRL were further supplemented with different β-glucan extracts. One was derived from S. cerevisiae (diet MG) and two different extracts of 21% and 37% P. tricornutum-derived β-glucans (defined as Phaeo21 and Phaeo37), to give a final 0.06% β-glucan dietary concentration. Quadruplicate groups of 95 gilthead seabream (initial body weight: 4.1 ± 0.1 g) were fed to satiation three times a day for 8 weeks in a pulse-feeding regimen, with experimental diets intercalated with the CTRL dietary treatment every 2 weeks. After 8 weeks of feeding, all groups showed equal growth performance and no changes were found in plasma innate immune status. Nonetheless, fish groups fed β-glucans supplemented diets showed an improved anti-oxidant status compared to those fed CTRL at both sampling points (i.e., 2 and 8 weeks). The intestinal gene expression analysis highlighted the immunomodulatory role of Phaeo37 diet after 8 weeks, inducing an immune tolerance effect in gilthead seabream intestine, and a general down-regulation of immune-related gene expression. In conclusion, the results suggest that the dietary pulse administration of a P. tricornutum 37% enriched-β-glucans extract might be used as a counter-measure in a context of gut inflammation, due to its immune-tolerant and anti-oxidative effects. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12 2021-12-01T00:00:00Z 2022-01-18T14:38:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/17493 |
url |
http://hdl.handle.net/10400.1/17493 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1660-3397 10.3390/md19120653 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133319840923648 |