Potato cyst nematodes infesting potato fields in Ecuador: integrative diagnosis and molecular phylogeny
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/28208 https://doi.org/doi: doi.org/10.1139/gen-2015-0087 |
Resumo: | Potato cyst nematodes (PCN), Globodera rostochiensis and G. pallida, are serious pests of potatoes worldwide, with some species included in the quarantine lists of many countries. Their accurate and timely identification is a prerequisite in designing effectivemanagement strategies. In Ecuador, potato is one of themain crops, and PCNrepresent an important pest. This situation led to a national surveywhere a total of 85 soil samples were collected in 2013 from the major potato-growing regions. The main objectives were to determine the prevalence of PCN and to establish a method for the integrative diagnosis of species based on the combination ofmorphological datawithmolecular analysis using rRNA regions. PCR-based techniques for species-specific amplification of the ITS region and for sequencing the D2–D3 expansion segments of the 28S rRNA gene were also developed. Results: Globodera pallida was the only PCN species found in the potato fields, but it was widespread (55% frequency of infestation).Moderate to highG. pallida population densities were found. The results from the molecular methods were congruent with the morphological data. Interspecific divergence of the D2–D3 regionof PCNis low, and it isnot possible to discriminate betweenG. pallida and G. rostrochiensis based upon thismolecular region, but it is useful for the distinction of other species. Phylogenetic analysis yielded twomajor well-defined and supported clades, where Ecuadorian sequences grouped with sequences of G. pallida and G. rostochiensis populations deposited in the GenBank database. Significance: The prevalence of G. pallidamakes it a severe threat to potato production in Ecuador.Moreover, management practices such as the short termof crop rotations and the use of the “super-chola” susceptible cultivar could be worsening the problem. A strategy based on the integration of morphological data and molecular analysis is useful for identifying PCN species. In addition, other molecular barcodes are currently being characterized (ITS-rRNA, COI-mitDNA) |
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Potato cyst nematodes infesting potato fields in Ecuador: integrative diagnosis and molecular phylogenycyst nematodesPotatoEcuadorrDNAPotato cyst nematodes (PCN), Globodera rostochiensis and G. pallida, are serious pests of potatoes worldwide, with some species included in the quarantine lists of many countries. Their accurate and timely identification is a prerequisite in designing effectivemanagement strategies. In Ecuador, potato is one of themain crops, and PCNrepresent an important pest. This situation led to a national surveywhere a total of 85 soil samples were collected in 2013 from the major potato-growing regions. The main objectives were to determine the prevalence of PCN and to establish a method for the integrative diagnosis of species based on the combination ofmorphological datawithmolecular analysis using rRNA regions. PCR-based techniques for species-specific amplification of the ITS region and for sequencing the D2–D3 expansion segments of the 28S rRNA gene were also developed. Results: Globodera pallida was the only PCN species found in the potato fields, but it was widespread (55% frequency of infestation).Moderate to highG. pallida population densities were found. The results from the molecular methods were congruent with the morphological data. Interspecific divergence of the D2–D3 regionof PCNis low, and it isnot possible to discriminate betweenG. pallida and G. rostrochiensis based upon thismolecular region, but it is useful for the distinction of other species. Phylogenetic analysis yielded twomajor well-defined and supported clades, where Ecuadorian sequences grouped with sequences of G. pallida and G. rostochiensis populations deposited in the GenBank database. Significance: The prevalence of G. pallidamakes it a severe threat to potato production in Ecuador.Moreover, management practices such as the short termof crop rotations and the use of the “super-chola” susceptible cultivar could be worsening the problem. A strategy based on the integration of morphological data and molecular analysis is useful for identifying PCN species. In addition, other molecular barcodes are currently being characterized (ITS-rRNA, COI-mitDNA)Published by NRC Research Press2020-10-23T11:31:19Z2020-10-232021-08-21T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/28208https://doi.org/doi: doi.org/10.1139/gen-2015-0087http://hdl.handle.net/10174/28208https://doi.org/doi: doi.org/10.1139/gen-2015-0087pornaonaosimcarlosg@uevora.ptndndndndndndndnd581GUTIÉRREZ-GUTIÉRREZ, CarlosLLUMIQUINGA, PabloGALLEGOS, PatricioORBE, KaterinaCASTILLO, NestorULLOA, JenniferGUANOTÁSIG, CarlosENRIQUEZ, WladimirPROAÑO, Karinainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:24:23Zoai:dspace.uevora.pt:10174/28208Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:18:08.992816Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Potato cyst nematodes infesting potato fields in Ecuador: integrative diagnosis and molecular phylogeny |
title |
Potato cyst nematodes infesting potato fields in Ecuador: integrative diagnosis and molecular phylogeny |
spellingShingle |
Potato cyst nematodes infesting potato fields in Ecuador: integrative diagnosis and molecular phylogeny GUTIÉRREZ-GUTIÉRREZ, Carlos cyst nematodes Potato Ecuador rDNA |
title_short |
Potato cyst nematodes infesting potato fields in Ecuador: integrative diagnosis and molecular phylogeny |
title_full |
Potato cyst nematodes infesting potato fields in Ecuador: integrative diagnosis and molecular phylogeny |
title_fullStr |
Potato cyst nematodes infesting potato fields in Ecuador: integrative diagnosis and molecular phylogeny |
title_full_unstemmed |
Potato cyst nematodes infesting potato fields in Ecuador: integrative diagnosis and molecular phylogeny |
title_sort |
Potato cyst nematodes infesting potato fields in Ecuador: integrative diagnosis and molecular phylogeny |
author |
GUTIÉRREZ-GUTIÉRREZ, Carlos |
author_facet |
GUTIÉRREZ-GUTIÉRREZ, Carlos LLUMIQUINGA, Pablo GALLEGOS, Patricio ORBE, Katerina CASTILLO, Nestor ULLOA, Jennifer GUANOTÁSIG, Carlos ENRIQUEZ, Wladimir PROAÑO, Karina |
author_role |
author |
author2 |
LLUMIQUINGA, Pablo GALLEGOS, Patricio ORBE, Katerina CASTILLO, Nestor ULLOA, Jennifer GUANOTÁSIG, Carlos ENRIQUEZ, Wladimir PROAÑO, Karina |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
GUTIÉRREZ-GUTIÉRREZ, Carlos LLUMIQUINGA, Pablo GALLEGOS, Patricio ORBE, Katerina CASTILLO, Nestor ULLOA, Jennifer GUANOTÁSIG, Carlos ENRIQUEZ, Wladimir PROAÑO, Karina |
dc.subject.por.fl_str_mv |
cyst nematodes Potato Ecuador rDNA |
topic |
cyst nematodes Potato Ecuador rDNA |
description |
Potato cyst nematodes (PCN), Globodera rostochiensis and G. pallida, are serious pests of potatoes worldwide, with some species included in the quarantine lists of many countries. Their accurate and timely identification is a prerequisite in designing effectivemanagement strategies. In Ecuador, potato is one of themain crops, and PCNrepresent an important pest. This situation led to a national surveywhere a total of 85 soil samples were collected in 2013 from the major potato-growing regions. The main objectives were to determine the prevalence of PCN and to establish a method for the integrative diagnosis of species based on the combination ofmorphological datawithmolecular analysis using rRNA regions. PCR-based techniques for species-specific amplification of the ITS region and for sequencing the D2–D3 expansion segments of the 28S rRNA gene were also developed. Results: Globodera pallida was the only PCN species found in the potato fields, but it was widespread (55% frequency of infestation).Moderate to highG. pallida population densities were found. The results from the molecular methods were congruent with the morphological data. Interspecific divergence of the D2–D3 regionof PCNis low, and it isnot possible to discriminate betweenG. pallida and G. rostrochiensis based upon thismolecular region, but it is useful for the distinction of other species. Phylogenetic analysis yielded twomajor well-defined and supported clades, where Ecuadorian sequences grouped with sequences of G. pallida and G. rostochiensis populations deposited in the GenBank database. Significance: The prevalence of G. pallidamakes it a severe threat to potato production in Ecuador.Moreover, management practices such as the short termof crop rotations and the use of the “super-chola” susceptible cultivar could be worsening the problem. A strategy based on the integration of morphological data and molecular analysis is useful for identifying PCN species. In addition, other molecular barcodes are currently being characterized (ITS-rRNA, COI-mitDNA) |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-23T11:31:19Z 2020-10-23 2021-08-21T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/28208 https://doi.org/doi: doi.org/10.1139/gen-2015-0087 http://hdl.handle.net/10174/28208 https://doi.org/doi: doi.org/10.1139/gen-2015-0087 |
url |
http://hdl.handle.net/10174/28208 https://doi.org/doi: doi.org/10.1139/gen-2015-0087 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
nao nao sim carlosg@uevora.pt nd nd nd nd nd nd nd nd 581 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Published by NRC Research Press |
publisher.none.fl_str_mv |
Published by NRC Research Press |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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