Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31601 |
Resumo: | The partitioning and purification of polygalacturonases (PG) produced by Aspergillus niger URM 5162 were investigated in aqueous two-phase systems (ATPS), formed by polyethylene glycol and phosphate salts (PEG/phosphate). To evaluate the effect of the 4 independent variables – molar mass of polyethylene glycol (PEG) (400–8000 g/mol – MPEG), PEG concentration (12.5–17.5%, w/w – CPEG), phosphate concentration (15–25%, w/w, CPHOS) and pH (6.0–8.0) – on the 4 response variables: partition coefficient (K), activity yield (Y), purification factor (PF) and selectivity (S), a factorial design (24) was used. The endo-polygalacturonases (endo-PG) and exo-polygalacturonase (exo-PG) were preferentially partitioned in the top phase. For endo- and exo-PG, the highest values for the response variables K (1.23 and 2.40), Y (74.04% and 17.97%), PF (8.18 and 1.98) and S (24.68 and 48.07), respectively, were obtained for a CPEG of 12.5% (w/w), MPEG of 8000 g/mol, and CPHOS of 25% (w/w) at pH 6.0. These conditions were considered the most suitable for the purification of PG produced by A. niger URM 5162. Furthermore, the most important independent variables for endo- and exo-PG were CPHOS and MPEG, respectively. All independent variables studied and their interactions significantly influenced the response variables. According to these results, the PEG/phosphate system is a useful cost-effective alternative for purification of PG produced by A. niger URM 5162. |
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Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase systemAspergillus nigerPartitionPEG/phosphate ATPSPolygalacturonasesPurification factorScience & TechnologyThe partitioning and purification of polygalacturonases (PG) produced by Aspergillus niger URM 5162 were investigated in aqueous two-phase systems (ATPS), formed by polyethylene glycol and phosphate salts (PEG/phosphate). To evaluate the effect of the 4 independent variables – molar mass of polyethylene glycol (PEG) (400–8000 g/mol – MPEG), PEG concentration (12.5–17.5%, w/w – CPEG), phosphate concentration (15–25%, w/w, CPHOS) and pH (6.0–8.0) – on the 4 response variables: partition coefficient (K), activity yield (Y), purification factor (PF) and selectivity (S), a factorial design (24) was used. The endo-polygalacturonases (endo-PG) and exo-polygalacturonase (exo-PG) were preferentially partitioned in the top phase. For endo- and exo-PG, the highest values for the response variables K (1.23 and 2.40), Y (74.04% and 17.97%), PF (8.18 and 1.98) and S (24.68 and 48.07), respectively, were obtained for a CPEG of 12.5% (w/w), MPEG of 8000 g/mol, and CPHOS of 25% (w/w) at pH 6.0. These conditions were considered the most suitable for the purification of PG produced by A. niger URM 5162. Furthermore, the most important independent variables for endo- and exo-PG were CPHOS and MPEG, respectively. All independent variables studied and their interactions significantly influenced the response variables. According to these results, the PEG/phosphate system is a useful cost-effective alternative for purification of PG produced by A. niger URM 5162.M.H.C. Maciel thanks to Conselho Nacional de Desenvolvimento Cientlfico e Tecnologico (CNPq, Brazil), Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE/APQ-0290-2.12/10), Financiadora de Estudos e Projetos (FINEP/ref. 2083/07) and Erasmus Mundus External Cooperation Window Lote 17. The authors thank the Laboratorio de Tecnologia de Bioativos (LABTECBIO), Federal Rural University of Pernambuco (Recife, Brazil). M.H.C. Maciel, C.A. Ottoni, C. Santos and N. Lima also thank the FCT Strategic Project PEst-OE/EQB/LA0023/2013 and the Project "BioEnv - Biotechnology and Bioengineering for a sustainable world", REF. NORTE-07-0124-FEDER-000048." Co-funded by the Programa Operacional Regional do Norte (ON.2-O Novo Norte), QREN, FEDER.Elsevier B.V.Universidade do MinhoMaciel, Marília de Holanda CavalcantiOttoni, C. A.Herculano, Polyanna N.Porto, Tatiana SouzaPorto, Ana L. F.Santos, CledirLima, NelsonMoreira, KeilaMotta, Cristina Maria de Souza20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31601engMaciel, M. H. C.; Ottoni, C. A.; Herculano, Polyanna N.; Porto, Tatiana S.; Porto, Ana L. F.; Santos, C.; Lima, Nelson; Moreira, Keila A.; Souza-Motta, Cristina, Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system. Fluid Phase Equilibria, 371, 125-130, 20140378-38120378-381210.1016/j.fluid.2014.03.018info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:00:43Zoai:repositorium.sdum.uminho.pt:1822/31601Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:50:34.931553Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system |
title |
Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system |
spellingShingle |
Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system Maciel, Marília de Holanda Cavalcanti Aspergillus niger Partition PEG/phosphate ATPS Polygalacturonases Purification factor Science & Technology |
title_short |
Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system |
title_full |
Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system |
title_fullStr |
Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system |
title_full_unstemmed |
Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system |
title_sort |
Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system |
author |
Maciel, Marília de Holanda Cavalcanti |
author_facet |
Maciel, Marília de Holanda Cavalcanti Ottoni, C. A. Herculano, Polyanna N. Porto, Tatiana Souza Porto, Ana L. F. Santos, Cledir Lima, Nelson Moreira, Keila Motta, Cristina Maria de Souza |
author_role |
author |
author2 |
Ottoni, C. A. Herculano, Polyanna N. Porto, Tatiana Souza Porto, Ana L. F. Santos, Cledir Lima, Nelson Moreira, Keila Motta, Cristina Maria de Souza |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Maciel, Marília de Holanda Cavalcanti Ottoni, C. A. Herculano, Polyanna N. Porto, Tatiana Souza Porto, Ana L. F. Santos, Cledir Lima, Nelson Moreira, Keila Motta, Cristina Maria de Souza |
dc.subject.por.fl_str_mv |
Aspergillus niger Partition PEG/phosphate ATPS Polygalacturonases Purification factor Science & Technology |
topic |
Aspergillus niger Partition PEG/phosphate ATPS Polygalacturonases Purification factor Science & Technology |
description |
The partitioning and purification of polygalacturonases (PG) produced by Aspergillus niger URM 5162 were investigated in aqueous two-phase systems (ATPS), formed by polyethylene glycol and phosphate salts (PEG/phosphate). To evaluate the effect of the 4 independent variables – molar mass of polyethylene glycol (PEG) (400–8000 g/mol – MPEG), PEG concentration (12.5–17.5%, w/w – CPEG), phosphate concentration (15–25%, w/w, CPHOS) and pH (6.0–8.0) – on the 4 response variables: partition coefficient (K), activity yield (Y), purification factor (PF) and selectivity (S), a factorial design (24) was used. The endo-polygalacturonases (endo-PG) and exo-polygalacturonase (exo-PG) were preferentially partitioned in the top phase. For endo- and exo-PG, the highest values for the response variables K (1.23 and 2.40), Y (74.04% and 17.97%), PF (8.18 and 1.98) and S (24.68 and 48.07), respectively, were obtained for a CPEG of 12.5% (w/w), MPEG of 8000 g/mol, and CPHOS of 25% (w/w) at pH 6.0. These conditions were considered the most suitable for the purification of PG produced by A. niger URM 5162. Furthermore, the most important independent variables for endo- and exo-PG were CPHOS and MPEG, respectively. All independent variables studied and their interactions significantly influenced the response variables. According to these results, the PEG/phosphate system is a useful cost-effective alternative for purification of PG produced by A. niger URM 5162. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31601 |
url |
http://hdl.handle.net/1822/31601 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Maciel, M. H. C.; Ottoni, C. A.; Herculano, Polyanna N.; Porto, Tatiana S.; Porto, Ana L. F.; Santos, C.; Lima, Nelson; Moreira, Keila A.; Souza-Motta, Cristina, Purification of polygalacturonases produced by Aspergillus niger using an aqueous two-phase system. Fluid Phase Equilibria, 371, 125-130, 2014 0378-3812 0378-3812 10.1016/j.fluid.2014.03.018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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