Antibacterial and antifungal activity of curcumin and methylene blue associated with laser on bacterial and fungal strains

• Autor(a) principal: Meneses Cardoso, Brenda Ellen
• Data de Publicação: 2023
• Outros Autores: Albuquerque Medeiros, Ana Andreza, Lopes Martins, Matheus, Araujo de Sousa, André Luca, dos Santos Alves, Nathanael, Costa Veras, Leiz Maria, Araújo, Alyne Rodrigues, Pinto, Antonione Santos Bezerra
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista Ciências em Saúde
• Texto Completo: https://portalrcs.hcitajuba.org.br/index.php/rcsfmit_zero/article/view/1454
• topic: Bacterial infections; Curcumin; Fungal Infections; In vitro technique; Microbial drug resistance; Photochemotherapy; Photosensitizer

Objective: To analyze the effect of methylene blue and 10% curcumin in fungi and bacteria through an in vitro study using photodynamic therapy (PDT). Methods: Curcumin and methylene blue were photosensitized by a Photon Lase III laser applied for 90 s in a dark environment within a laminar flow chamber. Enterococcus faecalis and Candida albicans strains were cultured and standardized. Then, a minimum inhibitory concentration (MIC) assay was conducted for these photosensitizers, with concentration variations and incubation to evaluate their antimicrobial activity. Results: With PDT, Curcumin had significant antibacterial activity against E. faecalis (MIC = 250 µg/mL). In contrast, methylene blue had antibacterial activity against E. faecalis (MIC < 12.5 µg/mL with PDT) and antifungal activity against C. albicans (MIC <12.5 µg/mL with or without PDT). Both agents showed greater efficacy in the presence of the laser. The results suggest that curcumin and methylene blue associated with laser may effectively treat microbial infections. Conclusion: Further research is needed to evaluate the efficacy and safety of using these agents in animal and human models and their effectiveness against different bacterial and fungal strains.