Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (Frullaniaceae, Marchantiophyta)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Botanica Brasilica |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000100019 |
Resumo: | ABSTRACT In bryophytes, establishment can occur by a sexual or asexual process, but the production of spores enables colonization of a wider range of habitats and substrates than can asexual propagules. Successful germination is critical for establishment in a new environment. This paper addresses germination and sporeling development in Frullania ericoides, a leafy liverwort species. Fresh spores were inoculated in vitro in different culture strengths of Knop’s nutrient solution (one-fourth strength, half strength, full strength, one and a half strength and double strength), in order to evaluate the effects of this solution on spore germination and on the development of external protonema. On the first assessment, spore germination was observed at all the concentrations. Germination was endosporic, with cell division and proliferation, resulting in a globular protonema, within the spore wall. Beginning at the fourth week, the development of tightly concave primordial leaves was observed in all but the double-strength medium. Throughout the period of study, the treatments with lower concentrations exhibited external protonema with greater lengths. The double-strength treatment was statistically different from other treatments in at least two parameters. The results of this study demonstrate the potential of in vitro culture techniques for bryophyte spore studies and germplasm preservation. |
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Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (Frullaniaceae, Marchantiophyta)endosporic developmentKnop’s mediumliverwortsnutrientsprotonemasporelingABSTRACT In bryophytes, establishment can occur by a sexual or asexual process, but the production of spores enables colonization of a wider range of habitats and substrates than can asexual propagules. Successful germination is critical for establishment in a new environment. This paper addresses germination and sporeling development in Frullania ericoides, a leafy liverwort species. Fresh spores were inoculated in vitro in different culture strengths of Knop’s nutrient solution (one-fourth strength, half strength, full strength, one and a half strength and double strength), in order to evaluate the effects of this solution on spore germination and on the development of external protonema. On the first assessment, spore germination was observed at all the concentrations. Germination was endosporic, with cell division and proliferation, resulting in a globular protonema, within the spore wall. Beginning at the fourth week, the development of tightly concave primordial leaves was observed in all but the double-strength medium. Throughout the period of study, the treatments with lower concentrations exhibited external protonema with greater lengths. The double-strength treatment was statistically different from other treatments in at least two parameters. The results of this study demonstrate the potential of in vitro culture techniques for bryophyte spore studies and germplasm preservation.Sociedade Botânica do Brasil2017-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000100019Acta Botanica Brasilica v.31 n.1 2017reponame:Acta Botanica Brasilicainstname:Sociedade Botânica do Brasil (SBB)instacron:SBB10.1590/0102-33062016abb0336info:eu-repo/semantics/openAccessSilva-e-Costa,Juliana da CostaLuizi-Ponzo,Andrea PereiraResende,Cristiano Ferrara dePeixoto,Paulo Henrique Pereiraeng2017-03-22T00:00:00Zoai:scielo:S0102-33062017000100019Revistahttps://www.scielo.br/j/abb/ONGhttps://old.scielo.br/oai/scielo-oai.phpacta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com1677-941X0102-3306opendoar:2017-03-22T00:00Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB)false |
dc.title.none.fl_str_mv |
Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (Frullaniaceae, Marchantiophyta) |
title |
Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (Frullaniaceae, Marchantiophyta) |
spellingShingle |
Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (Frullaniaceae, Marchantiophyta) Silva-e-Costa,Juliana da Costa endosporic development Knop’s medium liverworts nutrients protonema sporeling |
title_short |
Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (Frullaniaceae, Marchantiophyta) |
title_full |
Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (Frullaniaceae, Marchantiophyta) |
title_fullStr |
Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (Frullaniaceae, Marchantiophyta) |
title_full_unstemmed |
Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (Frullaniaceae, Marchantiophyta) |
title_sort |
Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (Frullaniaceae, Marchantiophyta) |
author |
Silva-e-Costa,Juliana da Costa |
author_facet |
Silva-e-Costa,Juliana da Costa Luizi-Ponzo,Andrea Pereira Resende,Cristiano Ferrara de Peixoto,Paulo Henrique Pereira |
author_role |
author |
author2 |
Luizi-Ponzo,Andrea Pereira Resende,Cristiano Ferrara de Peixoto,Paulo Henrique Pereira |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Silva-e-Costa,Juliana da Costa Luizi-Ponzo,Andrea Pereira Resende,Cristiano Ferrara de Peixoto,Paulo Henrique Pereira |
dc.subject.por.fl_str_mv |
endosporic development Knop’s medium liverworts nutrients protonema sporeling |
topic |
endosporic development Knop’s medium liverworts nutrients protonema sporeling |
description |
ABSTRACT In bryophytes, establishment can occur by a sexual or asexual process, but the production of spores enables colonization of a wider range of habitats and substrates than can asexual propagules. Successful germination is critical for establishment in a new environment. This paper addresses germination and sporeling development in Frullania ericoides, a leafy liverwort species. Fresh spores were inoculated in vitro in different culture strengths of Knop’s nutrient solution (one-fourth strength, half strength, full strength, one and a half strength and double strength), in order to evaluate the effects of this solution on spore germination and on the development of external protonema. On the first assessment, spore germination was observed at all the concentrations. Germination was endosporic, with cell division and proliferation, resulting in a globular protonema, within the spore wall. Beginning at the fourth week, the development of tightly concave primordial leaves was observed in all but the double-strength medium. Throughout the period of study, the treatments with lower concentrations exhibited external protonema with greater lengths. The double-strength treatment was statistically different from other treatments in at least two parameters. The results of this study demonstrate the potential of in vitro culture techniques for bryophyte spore studies and germplasm preservation. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000100019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000100019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0102-33062016abb0336 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Botânica do Brasil |
publisher.none.fl_str_mv |
Sociedade Botânica do Brasil |
dc.source.none.fl_str_mv |
Acta Botanica Brasilica v.31 n.1 2017 reponame:Acta Botanica Brasilica instname:Sociedade Botânica do Brasil (SBB) instacron:SBB |
instname_str |
Sociedade Botânica do Brasil (SBB) |
instacron_str |
SBB |
institution |
SBB |
reponame_str |
Acta Botanica Brasilica |
collection |
Acta Botanica Brasilica |
repository.name.fl_str_mv |
Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB) |
repository.mail.fl_str_mv |
acta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com |
_version_ |
1752126662472892416 |