Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS)

Detalhes bibliográficos
Autor(a) principal: Cruz,Andre Freire
Data de Publicação: 2004
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Botanica Brasilica
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062004000300007
Resumo: The storage structures of spores of Gigaspora margarita Becker & Hall, an arbuscular mycorrhizal fungus, were analyzed by a transmission electronic microscope (TEM) and electron energy loss spectroscopy (EELS). The spores were hydrated, cryofixed using high pressure freezing, submitted to freeze substitution, embedded and prepared for TEM observations. The cryotechniques revealed that the storage structures in the spores are composed of lipids, electron dense granules (EDG), protein bodies and glycogen particles. The EELS detected the presence of nitrogen (N) and carbon (C) in these storage structures and in Bacteria-like organisms (BLO). Some amount of P was detected in the vacuoles and in BLO. The TEM and EELS techniques indicate the presence of storage structures in the fungal spore, and some essential elements (N, P and C) in these structures.
id SBB-1_6c0025680badbc30b44491d5af3302dd
oai_identifier_str oai:scielo:S0102-33062004000300007
network_acronym_str SBB-1
network_name_str Acta Botanica Brasilica
repository_id_str
spelling Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS)arbuscular mycorrhizal fungiEELSstoragetransmission electronic microscopeThe storage structures of spores of Gigaspora margarita Becker & Hall, an arbuscular mycorrhizal fungus, were analyzed by a transmission electronic microscope (TEM) and electron energy loss spectroscopy (EELS). The spores were hydrated, cryofixed using high pressure freezing, submitted to freeze substitution, embedded and prepared for TEM observations. The cryotechniques revealed that the storage structures in the spores are composed of lipids, electron dense granules (EDG), protein bodies and glycogen particles. The EELS detected the presence of nitrogen (N) and carbon (C) in these storage structures and in Bacteria-like organisms (BLO). Some amount of P was detected in the vacuoles and in BLO. The TEM and EELS techniques indicate the presence of storage structures in the fungal spore, and some essential elements (N, P and C) in these structures.Sociedade Botânica do Brasil2004-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062004000300007Acta Botanica Brasilica v.18 n.3 2004reponame:Acta Botanica Brasilicainstname:Sociedade Botânica do Brasil (SBB)instacron:SBB10.1590/S0102-33062004000300007info:eu-repo/semantics/openAccessCruz,Andre Freireeng2005-02-02T00:00:00Zoai:scielo:S0102-33062004000300007Revistahttps://www.scielo.br/j/abb/ONGhttps://old.scielo.br/oai/scielo-oai.phpacta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com1677-941X0102-3306opendoar:2005-02-02T00:00Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB)false
dc.title.none.fl_str_mv Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS)
title Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS)
spellingShingle Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS)
Cruz,Andre Freire
arbuscular mycorrhizal fungi
EELS
storage
transmission electronic microscope
title_short Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS)
title_full Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS)
title_fullStr Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS)
title_full_unstemmed Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS)
title_sort Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS)
author Cruz,Andre Freire
author_facet Cruz,Andre Freire
author_role author
dc.contributor.author.fl_str_mv Cruz,Andre Freire
dc.subject.por.fl_str_mv arbuscular mycorrhizal fungi
EELS
storage
transmission electronic microscope
topic arbuscular mycorrhizal fungi
EELS
storage
transmission electronic microscope
description The storage structures of spores of Gigaspora margarita Becker & Hall, an arbuscular mycorrhizal fungus, were analyzed by a transmission electronic microscope (TEM) and electron energy loss spectroscopy (EELS). The spores were hydrated, cryofixed using high pressure freezing, submitted to freeze substitution, embedded and prepared for TEM observations. The cryotechniques revealed that the storage structures in the spores are composed of lipids, electron dense granules (EDG), protein bodies and glycogen particles. The EELS detected the presence of nitrogen (N) and carbon (C) in these storage structures and in Bacteria-like organisms (BLO). Some amount of P was detected in the vacuoles and in BLO. The TEM and EELS techniques indicate the presence of storage structures in the fungal spore, and some essential elements (N, P and C) in these structures.
publishDate 2004
dc.date.none.fl_str_mv 2004-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062004000300007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062004000300007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-33062004000300007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Botânica do Brasil
publisher.none.fl_str_mv Sociedade Botânica do Brasil
dc.source.none.fl_str_mv Acta Botanica Brasilica v.18 n.3 2004
reponame:Acta Botanica Brasilica
instname:Sociedade Botânica do Brasil (SBB)
instacron:SBB
instname_str Sociedade Botânica do Brasil (SBB)
instacron_str SBB
institution SBB
reponame_str Acta Botanica Brasilica
collection Acta Botanica Brasilica
repository.name.fl_str_mv Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB)
repository.mail.fl_str_mv acta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com
_version_ 1752126657170243584