Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS)
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Botanica Brasilica |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062004000300007 |
Resumo: | The storage structures of spores of Gigaspora margarita Becker & Hall, an arbuscular mycorrhizal fungus, were analyzed by a transmission electronic microscope (TEM) and electron energy loss spectroscopy (EELS). The spores were hydrated, cryofixed using high pressure freezing, submitted to freeze substitution, embedded and prepared for TEM observations. The cryotechniques revealed that the storage structures in the spores are composed of lipids, electron dense granules (EDG), protein bodies and glycogen particles. The EELS detected the presence of nitrogen (N) and carbon (C) in these storage structures and in Bacteria-like organisms (BLO). Some amount of P was detected in the vacuoles and in BLO. The TEM and EELS techniques indicate the presence of storage structures in the fungal spore, and some essential elements (N, P and C) in these structures. |
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Acta Botanica Brasilica |
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Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS)arbuscular mycorrhizal fungiEELSstoragetransmission electronic microscopeThe storage structures of spores of Gigaspora margarita Becker & Hall, an arbuscular mycorrhizal fungus, were analyzed by a transmission electronic microscope (TEM) and electron energy loss spectroscopy (EELS). The spores were hydrated, cryofixed using high pressure freezing, submitted to freeze substitution, embedded and prepared for TEM observations. The cryotechniques revealed that the storage structures in the spores are composed of lipids, electron dense granules (EDG), protein bodies and glycogen particles. The EELS detected the presence of nitrogen (N) and carbon (C) in these storage structures and in Bacteria-like organisms (BLO). Some amount of P was detected in the vacuoles and in BLO. The TEM and EELS techniques indicate the presence of storage structures in the fungal spore, and some essential elements (N, P and C) in these structures.Sociedade Botânica do Brasil2004-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062004000300007Acta Botanica Brasilica v.18 n.3 2004reponame:Acta Botanica Brasilicainstname:Sociedade Botânica do Brasil (SBB)instacron:SBB10.1590/S0102-33062004000300007info:eu-repo/semantics/openAccessCruz,Andre Freireeng2005-02-02T00:00:00Zoai:scielo:S0102-33062004000300007Revistahttps://www.scielo.br/j/abb/ONGhttps://old.scielo.br/oai/scielo-oai.phpacta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com1677-941X0102-3306opendoar:2005-02-02T00:00Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB)false |
dc.title.none.fl_str_mv |
Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS) |
title |
Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS) |
spellingShingle |
Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS) Cruz,Andre Freire arbuscular mycorrhizal fungi EELS storage transmission electronic microscope |
title_short |
Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS) |
title_full |
Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS) |
title_fullStr |
Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS) |
title_full_unstemmed |
Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS) |
title_sort |
Element storage in spores of Gigaspora margarita Becker & Hall measured by electron energy loss spectroscopy (EELS) |
author |
Cruz,Andre Freire |
author_facet |
Cruz,Andre Freire |
author_role |
author |
dc.contributor.author.fl_str_mv |
Cruz,Andre Freire |
dc.subject.por.fl_str_mv |
arbuscular mycorrhizal fungi EELS storage transmission electronic microscope |
topic |
arbuscular mycorrhizal fungi EELS storage transmission electronic microscope |
description |
The storage structures of spores of Gigaspora margarita Becker & Hall, an arbuscular mycorrhizal fungus, were analyzed by a transmission electronic microscope (TEM) and electron energy loss spectroscopy (EELS). The spores were hydrated, cryofixed using high pressure freezing, submitted to freeze substitution, embedded and prepared for TEM observations. The cryotechniques revealed that the storage structures in the spores are composed of lipids, electron dense granules (EDG), protein bodies and glycogen particles. The EELS detected the presence of nitrogen (N) and carbon (C) in these storage structures and in Bacteria-like organisms (BLO). Some amount of P was detected in the vacuoles and in BLO. The TEM and EELS techniques indicate the presence of storage structures in the fungal spore, and some essential elements (N, P and C) in these structures. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062004000300007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062004000300007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-33062004000300007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Botânica do Brasil |
publisher.none.fl_str_mv |
Sociedade Botânica do Brasil |
dc.source.none.fl_str_mv |
Acta Botanica Brasilica v.18 n.3 2004 reponame:Acta Botanica Brasilica instname:Sociedade Botânica do Brasil (SBB) instacron:SBB |
instname_str |
Sociedade Botânica do Brasil (SBB) |
instacron_str |
SBB |
institution |
SBB |
reponame_str |
Acta Botanica Brasilica |
collection |
Acta Botanica Brasilica |
repository.name.fl_str_mv |
Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB) |
repository.mail.fl_str_mv |
acta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com |
_version_ |
1752126657170243584 |