Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna

Detalhes bibliográficos
Autor(a) principal: Andrade,Guilherme Afonso Kessler de
Data de Publicação: 2022
Outros Autores: Bezerra,Jadson Diogo Pereira, Vargas,Maria Victória Magalhães de, Bernardes,Bruna Mota, Goulart,Sara Navarrete Bohi, Alves,Rodrigo Paidano, Küster,Mariele Cristine Tesche, Pereira,Antônio Batista, Victoria,Filipe de Carvalho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Botanica Brasilica
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062022000100101
Resumo: ABSTRACT Studies involving endophytic fungi isolated from endemic plants and their antibacterial potential are largely unknown in the Pampa biome. In this study, we identified endophytic fungi isolated from Kelissa brasiliensis (Iridaceae), an endemic species of the Brazilian Pampa, and assessed their antibacterial activity. Endophytic fungi were first grouped based on macro-and micro-morphology, and one representative of each morphospecies was analyzed using sequences from the internal transcribed spacer (ITS) rDNA region. We then tested the fungal extracts against laboratory isolates of Staphylococcus aureus and Escherichia coli for antibacterial activity. A total of 30 endophytes were isolated from the tissues of K. brasiliensis, with the majority from the leaves. Endophytes were then grouped into seven morphospecies based on their morphological features and one representative from each was selected for phylogenetic analysis. The inference from the ITS rDNA sequences identified the endophytes of the seven selected morphospecies as belonging to six taxonomic groups: Colletotrichum (two), Diaporthe (one), Epicoccum (one), Fusarium (one), and Pestalotiopsis (one). The endophyte extracts revealed better results against E. coli than S. aureus, although the extracts from Colletotrichum and Pestalotiopsis sp. were statistically similar to the control antibiotic. Our study is a basis for endophytic fungi studies in Pampa.
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spelling Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna“bibi-pintadinha”Brazilian biomeColletotrichumendophytic fungiPestalotiopsisABSTRACT Studies involving endophytic fungi isolated from endemic plants and their antibacterial potential are largely unknown in the Pampa biome. In this study, we identified endophytic fungi isolated from Kelissa brasiliensis (Iridaceae), an endemic species of the Brazilian Pampa, and assessed their antibacterial activity. Endophytic fungi were first grouped based on macro-and micro-morphology, and one representative of each morphospecies was analyzed using sequences from the internal transcribed spacer (ITS) rDNA region. We then tested the fungal extracts against laboratory isolates of Staphylococcus aureus and Escherichia coli for antibacterial activity. A total of 30 endophytes were isolated from the tissues of K. brasiliensis, with the majority from the leaves. Endophytes were then grouped into seven morphospecies based on their morphological features and one representative from each was selected for phylogenetic analysis. The inference from the ITS rDNA sequences identified the endophytes of the seven selected morphospecies as belonging to six taxonomic groups: Colletotrichum (two), Diaporthe (one), Epicoccum (one), Fusarium (one), and Pestalotiopsis (one). The endophyte extracts revealed better results against E. coli than S. aureus, although the extracts from Colletotrichum and Pestalotiopsis sp. were statistically similar to the control antibiotic. Our study is a basis for endophytic fungi studies in Pampa.Sociedade Botânica do Brasil2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062022000100101Acta Botanica Brasilica v.36 2022reponame:Acta Botanica Brasilicainstname:Sociedade Botânica do Brasil (SBB)instacron:SBB10.1590/0102-33062020abb0426info:eu-repo/semantics/openAccessAndrade,Guilherme Afonso Kessler deBezerra,Jadson Diogo PereiraVargas,Maria Victória Magalhães deBernardes,Bruna MotaGoulart,Sara Navarrete BohiAlves,Rodrigo PaidanoKüster,Mariele Cristine TeschePereira,Antônio BatistaVictoria,Filipe de Carvalhoeng2022-03-09T00:00:00Zoai:scielo:S0102-33062022000100101Revistahttps://www.scielo.br/j/abb/ONGhttps://old.scielo.br/oai/scielo-oai.phpacta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com1677-941X0102-3306opendoar:2022-03-09T00:00Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB)false
dc.title.none.fl_str_mv Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna
title Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna
spellingShingle Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna
Andrade,Guilherme Afonso Kessler de
“bibi-pintadinha”
Brazilian biome
Colletotrichum
endophytic fungi
Pestalotiopsis
title_short Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna
title_full Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna
title_fullStr Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna
title_full_unstemmed Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna
title_sort Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna
author Andrade,Guilherme Afonso Kessler de
author_facet Andrade,Guilherme Afonso Kessler de
Bezerra,Jadson Diogo Pereira
Vargas,Maria Victória Magalhães de
Bernardes,Bruna Mota
Goulart,Sara Navarrete Bohi
Alves,Rodrigo Paidano
Küster,Mariele Cristine Tesche
Pereira,Antônio Batista
Victoria,Filipe de Carvalho
author_role author
author2 Bezerra,Jadson Diogo Pereira
Vargas,Maria Victória Magalhães de
Bernardes,Bruna Mota
Goulart,Sara Navarrete Bohi
Alves,Rodrigo Paidano
Küster,Mariele Cristine Tesche
Pereira,Antônio Batista
Victoria,Filipe de Carvalho
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Andrade,Guilherme Afonso Kessler de
Bezerra,Jadson Diogo Pereira
Vargas,Maria Victória Magalhães de
Bernardes,Bruna Mota
Goulart,Sara Navarrete Bohi
Alves,Rodrigo Paidano
Küster,Mariele Cristine Tesche
Pereira,Antônio Batista
Victoria,Filipe de Carvalho
dc.subject.por.fl_str_mv “bibi-pintadinha”
Brazilian biome
Colletotrichum
endophytic fungi
Pestalotiopsis
topic “bibi-pintadinha”
Brazilian biome
Colletotrichum
endophytic fungi
Pestalotiopsis
description ABSTRACT Studies involving endophytic fungi isolated from endemic plants and their antibacterial potential are largely unknown in the Pampa biome. In this study, we identified endophytic fungi isolated from Kelissa brasiliensis (Iridaceae), an endemic species of the Brazilian Pampa, and assessed their antibacterial activity. Endophytic fungi were first grouped based on macro-and micro-morphology, and one representative of each morphospecies was analyzed using sequences from the internal transcribed spacer (ITS) rDNA region. We then tested the fungal extracts against laboratory isolates of Staphylococcus aureus and Escherichia coli for antibacterial activity. A total of 30 endophytes were isolated from the tissues of K. brasiliensis, with the majority from the leaves. Endophytes were then grouped into seven morphospecies based on their morphological features and one representative from each was selected for phylogenetic analysis. The inference from the ITS rDNA sequences identified the endophytes of the seven selected morphospecies as belonging to six taxonomic groups: Colletotrichum (two), Diaporthe (one), Epicoccum (one), Fusarium (one), and Pestalotiopsis (one). The endophyte extracts revealed better results against E. coli than S. aureus, although the extracts from Colletotrichum and Pestalotiopsis sp. were statistically similar to the control antibiotic. Our study is a basis for endophytic fungi studies in Pampa.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062022000100101
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062022000100101
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0102-33062020abb0426
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Botânica do Brasil
publisher.none.fl_str_mv Sociedade Botânica do Brasil
dc.source.none.fl_str_mv Acta Botanica Brasilica v.36 2022
reponame:Acta Botanica Brasilica
instname:Sociedade Botânica do Brasil (SBB)
instacron:SBB
instname_str Sociedade Botânica do Brasil (SBB)
instacron_str SBB
institution SBB
reponame_str Acta Botanica Brasilica
collection Acta Botanica Brasilica
repository.name.fl_str_mv Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB)
repository.mail.fl_str_mv acta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com
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