Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Botanica Brasilica |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062022000100101 |
Resumo: | ABSTRACT Studies involving endophytic fungi isolated from endemic plants and their antibacterial potential are largely unknown in the Pampa biome. In this study, we identified endophytic fungi isolated from Kelissa brasiliensis (Iridaceae), an endemic species of the Brazilian Pampa, and assessed their antibacterial activity. Endophytic fungi were first grouped based on macro-and micro-morphology, and one representative of each morphospecies was analyzed using sequences from the internal transcribed spacer (ITS) rDNA region. We then tested the fungal extracts against laboratory isolates of Staphylococcus aureus and Escherichia coli for antibacterial activity. A total of 30 endophytes were isolated from the tissues of K. brasiliensis, with the majority from the leaves. Endophytes were then grouped into seven morphospecies based on their morphological features and one representative from each was selected for phylogenetic analysis. The inference from the ITS rDNA sequences identified the endophytes of the seven selected morphospecies as belonging to six taxonomic groups: Colletotrichum (two), Diaporthe (one), Epicoccum (one), Fusarium (one), and Pestalotiopsis (one). The endophyte extracts revealed better results against E. coli than S. aureus, although the extracts from Colletotrichum and Pestalotiopsis sp. were statistically similar to the control antibiotic. Our study is a basis for endophytic fungi studies in Pampa. |
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Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna“bibi-pintadinha”Brazilian biomeColletotrichumendophytic fungiPestalotiopsisABSTRACT Studies involving endophytic fungi isolated from endemic plants and their antibacterial potential are largely unknown in the Pampa biome. In this study, we identified endophytic fungi isolated from Kelissa brasiliensis (Iridaceae), an endemic species of the Brazilian Pampa, and assessed their antibacterial activity. Endophytic fungi were first grouped based on macro-and micro-morphology, and one representative of each morphospecies was analyzed using sequences from the internal transcribed spacer (ITS) rDNA region. We then tested the fungal extracts against laboratory isolates of Staphylococcus aureus and Escherichia coli for antibacterial activity. A total of 30 endophytes were isolated from the tissues of K. brasiliensis, with the majority from the leaves. Endophytes were then grouped into seven morphospecies based on their morphological features and one representative from each was selected for phylogenetic analysis. The inference from the ITS rDNA sequences identified the endophytes of the seven selected morphospecies as belonging to six taxonomic groups: Colletotrichum (two), Diaporthe (one), Epicoccum (one), Fusarium (one), and Pestalotiopsis (one). The endophyte extracts revealed better results against E. coli than S. aureus, although the extracts from Colletotrichum and Pestalotiopsis sp. were statistically similar to the control antibiotic. Our study is a basis for endophytic fungi studies in Pampa.Sociedade Botânica do Brasil2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062022000100101Acta Botanica Brasilica v.36 2022reponame:Acta Botanica Brasilicainstname:Sociedade Botânica do Brasil (SBB)instacron:SBB10.1590/0102-33062020abb0426info:eu-repo/semantics/openAccessAndrade,Guilherme Afonso Kessler deBezerra,Jadson Diogo PereiraVargas,Maria Victória Magalhães deBernardes,Bruna MotaGoulart,Sara Navarrete BohiAlves,Rodrigo PaidanoKüster,Mariele Cristine TeschePereira,Antônio BatistaVictoria,Filipe de Carvalhoeng2022-03-09T00:00:00Zoai:scielo:S0102-33062022000100101Revistahttps://www.scielo.br/j/abb/ONGhttps://old.scielo.br/oai/scielo-oai.phpacta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com1677-941X0102-3306opendoar:2022-03-09T00:00Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB)false |
dc.title.none.fl_str_mv |
Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna |
title |
Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna |
spellingShingle |
Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna Andrade,Guilherme Afonso Kessler de “bibi-pintadinha” Brazilian biome Colletotrichum endophytic fungi Pestalotiopsis |
title_short |
Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna |
title_full |
Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna |
title_fullStr |
Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna |
title_full_unstemmed |
Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna |
title_sort |
Endophytic fungi from an overlooked plant species: A case study in Kelissa brasiliensis (Baker) Ravenna |
author |
Andrade,Guilherme Afonso Kessler de |
author_facet |
Andrade,Guilherme Afonso Kessler de Bezerra,Jadson Diogo Pereira Vargas,Maria Victória Magalhães de Bernardes,Bruna Mota Goulart,Sara Navarrete Bohi Alves,Rodrigo Paidano Küster,Mariele Cristine Tesche Pereira,Antônio Batista Victoria,Filipe de Carvalho |
author_role |
author |
author2 |
Bezerra,Jadson Diogo Pereira Vargas,Maria Victória Magalhães de Bernardes,Bruna Mota Goulart,Sara Navarrete Bohi Alves,Rodrigo Paidano Küster,Mariele Cristine Tesche Pereira,Antônio Batista Victoria,Filipe de Carvalho |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Andrade,Guilherme Afonso Kessler de Bezerra,Jadson Diogo Pereira Vargas,Maria Victória Magalhães de Bernardes,Bruna Mota Goulart,Sara Navarrete Bohi Alves,Rodrigo Paidano Küster,Mariele Cristine Tesche Pereira,Antônio Batista Victoria,Filipe de Carvalho |
dc.subject.por.fl_str_mv |
“bibi-pintadinha” Brazilian biome Colletotrichum endophytic fungi Pestalotiopsis |
topic |
“bibi-pintadinha” Brazilian biome Colletotrichum endophytic fungi Pestalotiopsis |
description |
ABSTRACT Studies involving endophytic fungi isolated from endemic plants and their antibacterial potential are largely unknown in the Pampa biome. In this study, we identified endophytic fungi isolated from Kelissa brasiliensis (Iridaceae), an endemic species of the Brazilian Pampa, and assessed their antibacterial activity. Endophytic fungi were first grouped based on macro-and micro-morphology, and one representative of each morphospecies was analyzed using sequences from the internal transcribed spacer (ITS) rDNA region. We then tested the fungal extracts against laboratory isolates of Staphylococcus aureus and Escherichia coli for antibacterial activity. A total of 30 endophytes were isolated from the tissues of K. brasiliensis, with the majority from the leaves. Endophytes were then grouped into seven morphospecies based on their morphological features and one representative from each was selected for phylogenetic analysis. The inference from the ITS rDNA sequences identified the endophytes of the seven selected morphospecies as belonging to six taxonomic groups: Colletotrichum (two), Diaporthe (one), Epicoccum (one), Fusarium (one), and Pestalotiopsis (one). The endophyte extracts revealed better results against E. coli than S. aureus, although the extracts from Colletotrichum and Pestalotiopsis sp. were statistically similar to the control antibiotic. Our study is a basis for endophytic fungi studies in Pampa. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062022000100101 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062022000100101 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0102-33062020abb0426 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Botânica do Brasil |
publisher.none.fl_str_mv |
Sociedade Botânica do Brasil |
dc.source.none.fl_str_mv |
Acta Botanica Brasilica v.36 2022 reponame:Acta Botanica Brasilica instname:Sociedade Botânica do Brasil (SBB) instacron:SBB |
instname_str |
Sociedade Botânica do Brasil (SBB) |
instacron_str |
SBB |
institution |
SBB |
reponame_str |
Acta Botanica Brasilica |
collection |
Acta Botanica Brasilica |
repository.name.fl_str_mv |
Acta Botanica Brasilica - Sociedade Botânica do Brasil (SBB) |
repository.mail.fl_str_mv |
acta@botanica.org.br||acta@botanica.org.br|| f.a.r.santos@gmail.com |
_version_ |
1752126664239742976 |