Virtual Biochemistry – pH effect on enzyme activity

Detalhes bibliográficos
Autor(a) principal: Heidrich, D.N.
Data de Publicação: 2011
Outros Autores: Antônio, R.V., Figueiredo, M.S.R.B., Sugai, J.K., Angotti, J.A.P.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Ensino de Bioquímica
Texto Completo: http://bioquimica.org.br/revista/ojs/index.php/REB/article/view/227
Resumo: Protocols of laboratory experiments, followed by teacher's explanation, not always clearly translate to the student the dynamics to beadopted for the implementation of the proposed practice. One of these cases is related to the study of the effect of pH on enzyme activity. For better help the understanding of the technical procedure, a hypermedia was built based on a protocol adopted at the Department of Biochemistry, UFSC. The hypermedia shows how theeffect of variations in pH can be observed  in vitro. Taking as example salivary amylase and the consumption of starch (substrate) by means of iodine staining, a set of pH buffers was tested to identify the best pH for this enzyme  activity. This hypermedia as introductory tool for such practice was tested on aNutrition course classroom. Students agree that the hypermedia provided a better understanding of the proposed activities. Teachers also notice a smallerreagents consumption and reduction of the time spent by the students in the achievement of the experiment.
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spelling Virtual Biochemistry – pH effect on enzyme activitybiochemical hypermedia, pH effect, salivary amylase, virtual experiment.Protocols of laboratory experiments, followed by teacher's explanation, not always clearly translate to the student the dynamics to beadopted for the implementation of the proposed practice. One of these cases is related to the study of the effect of pH on enzyme activity. For better help the understanding of the technical procedure, a hypermedia was built based on a protocol adopted at the Department of Biochemistry, UFSC. The hypermedia shows how theeffect of variations in pH can be observed  in vitro. Taking as example salivary amylase and the consumption of starch (substrate) by means of iodine staining, a set of pH buffers was tested to identify the best pH for this enzyme  activity. This hypermedia as introductory tool for such practice was tested on aNutrition course classroom. Students agree that the hypermedia provided a better understanding of the proposed activities. Teachers also notice a smallerreagents consumption and reduction of the time spent by the students in the achievement of the experiment.Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBqCNPq.Heidrich, D.N.Antônio, R.V.Figueiredo, M.S.R.B.Sugai, J.K.Angotti, J.A.P.2011-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResumos SBBq - avaliados pelos paresSBBq resúmenes - revisada por paresSBBq abstracts / peer-reviewedapplication/pdfhttp://bioquimica.org.br/revista/ojs/index.php/REB/article/view/22710.16923/reb.v9i2.227Revista de Ensino de Bioquímica; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 1Revista de Enseñanza de Bioquímica; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 1Journal of Biochemistry Education; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 1Revista de Ensino de Bioquímica; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 12318-8790reponame:Revista de Ensino de Bioquímicainstname:Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq)instacron:SBBQMporhttp://bioquimica.org.br/revista/ojs/index.php/REB/article/view/227/212Direitos autorais 2011 Revista de Ensino de Bioquímicahttp://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2022-03-25T17:17:54Zoai:ojs.bioquimica.org.br:article/227Revistahttp://bioquimica.org.br/revista/ojs/index.php/REBONGhttp://bioquimica.org.br/revista/ojs/index.php/REB/oaicontato@bioquimica.org.br||ensinodebioquimica@gmail.com2318-87901677-2318opendoar:2022-03-25T17:17:54Revista de Ensino de Bioquímica - Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq)false
dc.title.none.fl_str_mv Virtual Biochemistry – pH effect on enzyme activity
title Virtual Biochemistry – pH effect on enzyme activity
spellingShingle Virtual Biochemistry – pH effect on enzyme activity
Heidrich, D.N.
biochemical hypermedia, pH effect, salivary amylase, virtual experiment.
title_short Virtual Biochemistry – pH effect on enzyme activity
title_full Virtual Biochemistry – pH effect on enzyme activity
title_fullStr Virtual Biochemistry – pH effect on enzyme activity
title_full_unstemmed Virtual Biochemistry – pH effect on enzyme activity
title_sort Virtual Biochemistry – pH effect on enzyme activity
author Heidrich, D.N.
author_facet Heidrich, D.N.
Antônio, R.V.
Figueiredo, M.S.R.B.
Sugai, J.K.
Angotti, J.A.P.
author_role author
author2 Antônio, R.V.
Figueiredo, M.S.R.B.
Sugai, J.K.
Angotti, J.A.P.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv CNPq.
dc.contributor.author.fl_str_mv Heidrich, D.N.
Antônio, R.V.
Figueiredo, M.S.R.B.
Sugai, J.K.
Angotti, J.A.P.
dc.subject.por.fl_str_mv biochemical hypermedia, pH effect, salivary amylase, virtual experiment.
topic biochemical hypermedia, pH effect, salivary amylase, virtual experiment.
description Protocols of laboratory experiments, followed by teacher's explanation, not always clearly translate to the student the dynamics to beadopted for the implementation of the proposed practice. One of these cases is related to the study of the effect of pH on enzyme activity. For better help the understanding of the technical procedure, a hypermedia was built based on a protocol adopted at the Department of Biochemistry, UFSC. The hypermedia shows how theeffect of variations in pH can be observed  in vitro. Taking as example salivary amylase and the consumption of starch (substrate) by means of iodine staining, a set of pH buffers was tested to identify the best pH for this enzyme  activity. This hypermedia as introductory tool for such practice was tested on aNutrition course classroom. Students agree that the hypermedia provided a better understanding of the proposed activities. Teachers also notice a smallerreagents consumption and reduction of the time spent by the students in the achievement of the experiment.
publishDate 2011
dc.date.none.fl_str_mv 2011-04-30
dc.type.none.fl_str_mv

dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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Resumos SBBq - avaliados pelos pares
SBBq resúmenes - revisada por pares
SBBq abstracts / peer-reviewed
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://bioquimica.org.br/revista/ojs/index.php/REB/article/view/227
10.16923/reb.v9i2.227
url http://bioquimica.org.br/revista/ojs/index.php/REB/article/view/227
identifier_str_mv 10.16923/reb.v9i2.227
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://bioquimica.org.br/revista/ojs/index.php/REB/article/view/227/212
dc.rights.driver.fl_str_mv Direitos autorais 2011 Revista de Ensino de Bioquímica
http://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Direitos autorais 2011 Revista de Ensino de Bioquímica
http://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq
publisher.none.fl_str_mv Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq
dc.source.none.fl_str_mv Revista de Ensino de Bioquímica; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 1
Revista de Enseñanza de Bioquímica; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 1
Journal of Biochemistry Education; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 1
Revista de Ensino de Bioquímica; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 1
2318-8790
reponame:Revista de Ensino de Bioquímica
instname:Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq)
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instname_str Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq)
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