Virtual Biochemistry – pH effect on enzyme activity
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Ensino de Bioquímica |
Texto Completo: | http://bioquimica.org.br/revista/ojs/index.php/REB/article/view/227 |
Resumo: | Protocols of laboratory experiments, followed by teacher's explanation, not always clearly translate to the student the dynamics to beadopted for the implementation of the proposed practice. One of these cases is related to the study of the effect of pH on enzyme activity. For better help the understanding of the technical procedure, a hypermedia was built based on a protocol adopted at the Department of Biochemistry, UFSC. The hypermedia shows how theeffect of variations in pH can be observed in vitro. Taking as example salivary amylase and the consumption of starch (substrate) by means of iodine staining, a set of pH buffers was tested to identify the best pH for this enzyme activity. This hypermedia as introductory tool for such practice was tested on aNutrition course classroom. Students agree that the hypermedia provided a better understanding of the proposed activities. Teachers also notice a smallerreagents consumption and reduction of the time spent by the students in the achievement of the experiment. |
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Virtual Biochemistry – pH effect on enzyme activitybiochemical hypermedia, pH effect, salivary amylase, virtual experiment.Protocols of laboratory experiments, followed by teacher's explanation, not always clearly translate to the student the dynamics to beadopted for the implementation of the proposed practice. One of these cases is related to the study of the effect of pH on enzyme activity. For better help the understanding of the technical procedure, a hypermedia was built based on a protocol adopted at the Department of Biochemistry, UFSC. The hypermedia shows how theeffect of variations in pH can be observed in vitro. Taking as example salivary amylase and the consumption of starch (substrate) by means of iodine staining, a set of pH buffers was tested to identify the best pH for this enzyme activity. This hypermedia as introductory tool for such practice was tested on aNutrition course classroom. Students agree that the hypermedia provided a better understanding of the proposed activities. Teachers also notice a smallerreagents consumption and reduction of the time spent by the students in the achievement of the experiment.Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBqCNPq.Heidrich, D.N.Antônio, R.V.Figueiredo, M.S.R.B.Sugai, J.K.Angotti, J.A.P.2011-04-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResumos SBBq - avaliados pelos paresSBBq resúmenes - revisada por paresSBBq abstracts / peer-reviewedapplication/pdfhttp://bioquimica.org.br/revista/ojs/index.php/REB/article/view/22710.16923/reb.v9i2.227Revista de Ensino de Bioquímica; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 1Revista de Enseñanza de Bioquímica; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 1Journal of Biochemistry Education; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 1Revista de Ensino de Bioquímica; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 12318-8790reponame:Revista de Ensino de Bioquímicainstname:Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq)instacron:SBBQMporhttp://bioquimica.org.br/revista/ojs/index.php/REB/article/view/227/212Direitos autorais 2011 Revista de Ensino de Bioquímicahttp://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccess2022-03-25T17:17:54Zoai:ojs.bioquimica.org.br:article/227Revistahttp://bioquimica.org.br/revista/ojs/index.php/REBONGhttp://bioquimica.org.br/revista/ojs/index.php/REB/oaicontato@bioquimica.org.br||ensinodebioquimica@gmail.com2318-87901677-2318opendoar:2022-03-25T17:17:54Revista de Ensino de Bioquímica - Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq)false |
dc.title.none.fl_str_mv |
Virtual Biochemistry – pH effect on enzyme activity |
title |
Virtual Biochemistry – pH effect on enzyme activity |
spellingShingle |
Virtual Biochemistry – pH effect on enzyme activity Heidrich, D.N. biochemical hypermedia, pH effect, salivary amylase, virtual experiment. |
title_short |
Virtual Biochemistry – pH effect on enzyme activity |
title_full |
Virtual Biochemistry – pH effect on enzyme activity |
title_fullStr |
Virtual Biochemistry – pH effect on enzyme activity |
title_full_unstemmed |
Virtual Biochemistry – pH effect on enzyme activity |
title_sort |
Virtual Biochemistry – pH effect on enzyme activity |
author |
Heidrich, D.N. |
author_facet |
Heidrich, D.N. Antônio, R.V. Figueiredo, M.S.R.B. Sugai, J.K. Angotti, J.A.P. |
author_role |
author |
author2 |
Antônio, R.V. Figueiredo, M.S.R.B. Sugai, J.K. Angotti, J.A.P. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
CNPq. |
dc.contributor.author.fl_str_mv |
Heidrich, D.N. Antônio, R.V. Figueiredo, M.S.R.B. Sugai, J.K. Angotti, J.A.P. |
dc.subject.por.fl_str_mv |
biochemical hypermedia, pH effect, salivary amylase, virtual experiment. |
topic |
biochemical hypermedia, pH effect, salivary amylase, virtual experiment. |
description |
Protocols of laboratory experiments, followed by teacher's explanation, not always clearly translate to the student the dynamics to beadopted for the implementation of the proposed practice. One of these cases is related to the study of the effect of pH on enzyme activity. For better help the understanding of the technical procedure, a hypermedia was built based on a protocol adopted at the Department of Biochemistry, UFSC. The hypermedia shows how theeffect of variations in pH can be observed in vitro. Taking as example salivary amylase and the consumption of starch (substrate) by means of iodine staining, a set of pH buffers was tested to identify the best pH for this enzyme activity. This hypermedia as introductory tool for such practice was tested on aNutrition course classroom. Students agree that the hypermedia provided a better understanding of the proposed activities. Teachers also notice a smallerreagents consumption and reduction of the time spent by the students in the achievement of the experiment. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-04-30 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Resumos SBBq - avaliados pelos pares SBBq resúmenes - revisada por pares SBBq abstracts / peer-reviewed |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://bioquimica.org.br/revista/ojs/index.php/REB/article/view/227 10.16923/reb.v9i2.227 |
url |
http://bioquimica.org.br/revista/ojs/index.php/REB/article/view/227 |
identifier_str_mv |
10.16923/reb.v9i2.227 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://bioquimica.org.br/revista/ojs/index.php/REB/article/view/227/212 |
dc.rights.driver.fl_str_mv |
Direitos autorais 2011 Revista de Ensino de Bioquímica http://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos autorais 2011 Revista de Ensino de Bioquímica http://creativecommons.org/licenses/by-nc-sa/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq |
publisher.none.fl_str_mv |
Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq |
dc.source.none.fl_str_mv |
Revista de Ensino de Bioquímica; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 1 Revista de Enseñanza de Bioquímica; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 1 Journal of Biochemistry Education; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 1 Revista de Ensino de Bioquímica; v. 9, n. 2 (2011): Especial 11 (SBBq): RBEBBM; 1 2318-8790 reponame:Revista de Ensino de Bioquímica instname:Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq) instacron:SBBQM |
instname_str |
Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq) |
instacron_str |
SBBQM |
institution |
SBBQM |
reponame_str |
Revista de Ensino de Bioquímica |
collection |
Revista de Ensino de Bioquímica |
repository.name.fl_str_mv |
Revista de Ensino de Bioquímica - Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq) |
repository.mail.fl_str_mv |
contato@bioquimica.org.br||ensinodebioquimica@gmail.com |
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