Chlamydia pneumoniae and atherosclerosis. Identification of bacterial DNA in the arterial wall
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos Brasileiros de Cardiologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2000000200002 |
Resumo: | OBJECTIVE: The intracellular Gram-negative bacterium Chlamydia pneumoniae has been associated with atherosclerosis. The presence of Chlamydia pneumoniae has been investigated in fragments of the arterial wall with a technique for DNA identification. METHODS: Arterial fragments obtained from vascular surgical procedures in 58 patients were analyzed. From these patients, 39 were males and the mean age was 65±6 years. The polymerase chain reaction was used to identify the bacterial DNA with a pair of primers that codify the major outer membrane protein (MOMP) of Chlamydia pneumoniae. The amplified product was visualized by electrophoresis in the 2% agarose gel stained with ethidium bromide, and it was considered positive when migrating in the band of molecular weight of the positive controls. RESULTS: Seven (12%) out of the 58 patients showed positive results for Chlamydia pneumoniae. CONCLUSION: DNA from Chlamydia pneumoniae was identified in the arterial wall of a substantial number of patients with atherosclerosis. This association, which has already been described in other countries, corroborates the evidence favoring a role played by Chlamydia pneumoniae in atherogenesis. |
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Arquivos Brasileiros de Cardiologia (Online) |
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Chlamydia pneumoniae and atherosclerosis. Identification of bacterial DNA in the arterial wallChlamydia pneumoniaeatherosclerosispolymerase chain reactionOBJECTIVE: The intracellular Gram-negative bacterium Chlamydia pneumoniae has been associated with atherosclerosis. The presence of Chlamydia pneumoniae has been investigated in fragments of the arterial wall with a technique for DNA identification. METHODS: Arterial fragments obtained from vascular surgical procedures in 58 patients were analyzed. From these patients, 39 were males and the mean age was 65±6 years. The polymerase chain reaction was used to identify the bacterial DNA with a pair of primers that codify the major outer membrane protein (MOMP) of Chlamydia pneumoniae. The amplified product was visualized by electrophoresis in the 2% agarose gel stained with ethidium bromide, and it was considered positive when migrating in the band of molecular weight of the positive controls. RESULTS: Seven (12%) out of the 58 patients showed positive results for Chlamydia pneumoniae. CONCLUSION: DNA from Chlamydia pneumoniae was identified in the arterial wall of a substantial number of patients with atherosclerosis. This association, which has already been described in other countries, corroborates the evidence favoring a role played by Chlamydia pneumoniae in atherogenesis.Sociedade Brasileira de Cardiologia - SBC2000-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2000000200002Arquivos Brasileiros de Cardiologia v.74 n.2 2000reponame:Arquivos Brasileiros de Cardiologia (Online)instname:Sociedade Brasileira de Cardiologia (SBC)instacron:SBC10.1590/S0066-782X2000000200002info:eu-repo/semantics/openAccessCoutinho,Mário Sérgio Soares de AzeredoNakamae,Fernando Joo DanielMenezes,Maria Elizabetheng2000-09-05T00:00:00Zoai:scielo:S0066-782X2000000200002Revistahttp://www.arquivosonline.com.br/https://old.scielo.br/oai/scielo-oai.php||arquivos@cardiol.br1678-41700066-782Xopendoar:2000-09-05T00:00Arquivos Brasileiros de Cardiologia (Online) - Sociedade Brasileira de Cardiologia (SBC)false |
dc.title.none.fl_str_mv |
Chlamydia pneumoniae and atherosclerosis. Identification of bacterial DNA in the arterial wall |
title |
Chlamydia pneumoniae and atherosclerosis. Identification of bacterial DNA in the arterial wall |
spellingShingle |
Chlamydia pneumoniae and atherosclerosis. Identification of bacterial DNA in the arterial wall Coutinho,Mário Sérgio Soares de Azeredo Chlamydia pneumoniae atherosclerosis polymerase chain reaction |
title_short |
Chlamydia pneumoniae and atherosclerosis. Identification of bacterial DNA in the arterial wall |
title_full |
Chlamydia pneumoniae and atherosclerosis. Identification of bacterial DNA in the arterial wall |
title_fullStr |
Chlamydia pneumoniae and atherosclerosis. Identification of bacterial DNA in the arterial wall |
title_full_unstemmed |
Chlamydia pneumoniae and atherosclerosis. Identification of bacterial DNA in the arterial wall |
title_sort |
Chlamydia pneumoniae and atherosclerosis. Identification of bacterial DNA in the arterial wall |
author |
Coutinho,Mário Sérgio Soares de Azeredo |
author_facet |
Coutinho,Mário Sérgio Soares de Azeredo Nakamae,Fernando Joo Daniel Menezes,Maria Elizabeth |
author_role |
author |
author2 |
Nakamae,Fernando Joo Daniel Menezes,Maria Elizabeth |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Coutinho,Mário Sérgio Soares de Azeredo Nakamae,Fernando Joo Daniel Menezes,Maria Elizabeth |
dc.subject.por.fl_str_mv |
Chlamydia pneumoniae atherosclerosis polymerase chain reaction |
topic |
Chlamydia pneumoniae atherosclerosis polymerase chain reaction |
description |
OBJECTIVE: The intracellular Gram-negative bacterium Chlamydia pneumoniae has been associated with atherosclerosis. The presence of Chlamydia pneumoniae has been investigated in fragments of the arterial wall with a technique for DNA identification. METHODS: Arterial fragments obtained from vascular surgical procedures in 58 patients were analyzed. From these patients, 39 were males and the mean age was 65±6 years. The polymerase chain reaction was used to identify the bacterial DNA with a pair of primers that codify the major outer membrane protein (MOMP) of Chlamydia pneumoniae. The amplified product was visualized by electrophoresis in the 2% agarose gel stained with ethidium bromide, and it was considered positive when migrating in the band of molecular weight of the positive controls. RESULTS: Seven (12%) out of the 58 patients showed positive results for Chlamydia pneumoniae. CONCLUSION: DNA from Chlamydia pneumoniae was identified in the arterial wall of a substantial number of patients with atherosclerosis. This association, which has already been described in other countries, corroborates the evidence favoring a role played by Chlamydia pneumoniae in atherogenesis. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2000000200002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2000000200002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0066-782X2000000200002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Cardiologia - SBC |
publisher.none.fl_str_mv |
Sociedade Brasileira de Cardiologia - SBC |
dc.source.none.fl_str_mv |
Arquivos Brasileiros de Cardiologia v.74 n.2 2000 reponame:Arquivos Brasileiros de Cardiologia (Online) instname:Sociedade Brasileira de Cardiologia (SBC) instacron:SBC |
instname_str |
Sociedade Brasileira de Cardiologia (SBC) |
instacron_str |
SBC |
institution |
SBC |
reponame_str |
Arquivos Brasileiros de Cardiologia (Online) |
collection |
Arquivos Brasileiros de Cardiologia (Online) |
repository.name.fl_str_mv |
Arquivos Brasileiros de Cardiologia (Online) - Sociedade Brasileira de Cardiologia (SBC) |
repository.mail.fl_str_mv |
||arquivos@cardiol.br |
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1752126551234707456 |