One-tube nested Polymerase Chain Reaction for detection of Chlamydia trachomatis

Detalhes bibliográficos
Autor(a) principal: Cribb,Pamela
Data de Publicação: 2002
Outros Autores: Scapini,Juan Pablo, Serra,Esteban
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600027
Resumo: Here we present a one-tube nested PCR test, which allows the detection of minimal quantities of Chlamydia trachomatis in human fluids. This assay includes the use of an internal control to avoid false negative results due to the presence of inhibitors. The results obtained show that this assay is robust enough to be used for clinical diagnosis.
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spelling One-tube nested Polymerase Chain Reaction for detection of Chlamydia trachomatisChlamydiaPolymerase Chain ReactiondiagnosticHere we present a one-tube nested PCR test, which allows the detection of minimal quantities of Chlamydia trachomatis in human fluids. This assay includes the use of an internal control to avoid false negative results due to the presence of inhibitors. The results obtained show that this assay is robust enough to be used for clinical diagnosis.Instituto Oswaldo Cruz, Ministério da Saúde2002-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600027Memórias do Instituto Oswaldo Cruz v.97 n.6 2002reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762002000600027info:eu-repo/semantics/openAccessCribb,PamelaScapini,Juan PabloSerra,Estebaneng2020-04-25T17:48:51Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:11:22.434Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv One-tube nested Polymerase Chain Reaction for detection of Chlamydia trachomatis
title One-tube nested Polymerase Chain Reaction for detection of Chlamydia trachomatis
spellingShingle One-tube nested Polymerase Chain Reaction for detection of Chlamydia trachomatis
Cribb,Pamela
Chlamydia
Polymerase Chain Reaction
diagnostic
title_short One-tube nested Polymerase Chain Reaction for detection of Chlamydia trachomatis
title_full One-tube nested Polymerase Chain Reaction for detection of Chlamydia trachomatis
title_fullStr One-tube nested Polymerase Chain Reaction for detection of Chlamydia trachomatis
title_full_unstemmed One-tube nested Polymerase Chain Reaction for detection of Chlamydia trachomatis
title_sort One-tube nested Polymerase Chain Reaction for detection of Chlamydia trachomatis
author Cribb,Pamela
author_facet Cribb,Pamela
Scapini,Juan Pablo
Serra,Esteban
author_role author
author2 Scapini,Juan Pablo
Serra,Esteban
author2_role author
author
dc.contributor.author.fl_str_mv Cribb,Pamela
Scapini,Juan Pablo
Serra,Esteban
dc.subject.por.fl_str_mv Chlamydia
Polymerase Chain Reaction
diagnostic
topic Chlamydia
Polymerase Chain Reaction
diagnostic
dc.description.none.fl_txt_mv Here we present a one-tube nested PCR test, which allows the detection of minimal quantities of Chlamydia trachomatis in human fluids. This assay includes the use of an internal control to avoid false negative results due to the presence of inhibitors. The results obtained show that this assay is robust enough to be used for clinical diagnosis.
description Here we present a one-tube nested PCR test, which allows the detection of minimal quantities of Chlamydia trachomatis in human fluids. This assay includes the use of an internal control to avoid false negative results due to the presence of inhibitors. The results obtained show that this assay is robust enough to be used for clinical diagnosis.
publishDate 2002
dc.date.none.fl_str_mv 2002-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600027
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000600027
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762002000600027
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.97 n.6 2002
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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