Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Arquivos Brasileiros de Cardiologia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2018001400172 |
Resumo: | Abstract Background: Regulation of intracellular calcium (Ca2+) in cardiomyocytes is altered by hypertension; and aerobic exercise brings benefits to hypertensive individuals. Objective: To verify the effects of aerobic exercise training on contractility and intracellular calcium (Ca2+) transients of cardiomyocytes and on the expression of microRNA 214 (miR-214) in the left ventricle of spontaneously hypertensive rats (SHR). Methods: SHR and normotensive Wistar rats of 16 weeks were divided into 4 groups -sedentary hypertensive (SH); trained hypertensive (TH); sedentary normotensive (SN); and trained normotensive (TN). Animals of the TH and TN groups were subjected to treadmill running program, 5 days/week, 1 hour/day at 60-70% of maximum running velocity for 8 weeks. We adopted a p ≤ 0.05 as significance level for all comparisons. Results: Exercise training reduced systolic arterial pressure in hypertensive rats. In normotensive rats, exercise training reduced the time to 50% cell relaxation and the time to peak contraction and increased the time to 50% decay of the intracellular Ca2+ transients. In SHR, exercise increased the amplitude and reduced the time to 50% decay of Ca2+ transients. Exercise training increased the expression of miR-214 in hypertensive rats only. Conclusion: The aerobic training applied in this study increased the availability of intracellular Ca2+ and accelerated the sequestration of these ions in left ventricular myocytes of hypertensive rats, despite increased expression of miR-214 and maintenance of cell contractility. |
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Arquivos Brasileiros de Cardiologia (Online) |
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Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive RatsHypertensionExerciseRatsCalcium SignalingIntracellular CalciumSensing ProteinsAbstract Background: Regulation of intracellular calcium (Ca2+) in cardiomyocytes is altered by hypertension; and aerobic exercise brings benefits to hypertensive individuals. Objective: To verify the effects of aerobic exercise training on contractility and intracellular calcium (Ca2+) transients of cardiomyocytes and on the expression of microRNA 214 (miR-214) in the left ventricle of spontaneously hypertensive rats (SHR). Methods: SHR and normotensive Wistar rats of 16 weeks were divided into 4 groups -sedentary hypertensive (SH); trained hypertensive (TH); sedentary normotensive (SN); and trained normotensive (TN). Animals of the TH and TN groups were subjected to treadmill running program, 5 days/week, 1 hour/day at 60-70% of maximum running velocity for 8 weeks. We adopted a p ≤ 0.05 as significance level for all comparisons. Results: Exercise training reduced systolic arterial pressure in hypertensive rats. In normotensive rats, exercise training reduced the time to 50% cell relaxation and the time to peak contraction and increased the time to 50% decay of the intracellular Ca2+ transients. In SHR, exercise increased the amplitude and reduced the time to 50% decay of Ca2+ transients. Exercise training increased the expression of miR-214 in hypertensive rats only. Conclusion: The aerobic training applied in this study increased the availability of intracellular Ca2+ and accelerated the sequestration of these ions in left ventricular myocytes of hypertensive rats, despite increased expression of miR-214 and maintenance of cell contractility.Sociedade Brasileira de Cardiologia - SBC2018-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2018001400172Arquivos Brasileiros de Cardiologia v.111 n.2 2018reponame:Arquivos Brasileiros de Cardiologia (Online)instname:Sociedade Brasileira de Cardiologia (SBC)instacron:SBC10.5935/abc.20180113info:eu-repo/semantics/openAccessRodrigues,Joel AlvesPrímola-Gomes,Thales NicolauSoares,Leôncio LopesLeal,Tiago FerreiraNóbrega,ClaraPedrosa,Danillo LaviolaRezende,Leonardo Mateus TeixeiraOliveira,Edilamar Menezes deNatali,Antonio Joseeng2019-01-17T00:00:00Zoai:scielo:S0066-782X2018001400172Revistahttp://www.arquivosonline.com.br/https://old.scielo.br/oai/scielo-oai.php||arquivos@cardiol.br1678-41700066-782Xopendoar:2019-01-17T00:00Arquivos Brasileiros de Cardiologia (Online) - Sociedade Brasileira de Cardiologia (SBC)false |
dc.title.none.fl_str_mv |
Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats |
title |
Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats |
spellingShingle |
Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats Rodrigues,Joel Alves Hypertension Exercise Rats Calcium Signaling Intracellular Calcium Sensing Proteins |
title_short |
Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats |
title_full |
Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats |
title_fullStr |
Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats |
title_full_unstemmed |
Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats |
title_sort |
Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats |
author |
Rodrigues,Joel Alves |
author_facet |
Rodrigues,Joel Alves Prímola-Gomes,Thales Nicolau Soares,Leôncio Lopes Leal,Tiago Ferreira Nóbrega,Clara Pedrosa,Danillo Laviola Rezende,Leonardo Mateus Teixeira Oliveira,Edilamar Menezes de Natali,Antonio Jose |
author_role |
author |
author2 |
Prímola-Gomes,Thales Nicolau Soares,Leôncio Lopes Leal,Tiago Ferreira Nóbrega,Clara Pedrosa,Danillo Laviola Rezende,Leonardo Mateus Teixeira Oliveira,Edilamar Menezes de Natali,Antonio Jose |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues,Joel Alves Prímola-Gomes,Thales Nicolau Soares,Leôncio Lopes Leal,Tiago Ferreira Nóbrega,Clara Pedrosa,Danillo Laviola Rezende,Leonardo Mateus Teixeira Oliveira,Edilamar Menezes de Natali,Antonio Jose |
dc.subject.por.fl_str_mv |
Hypertension Exercise Rats Calcium Signaling Intracellular Calcium Sensing Proteins |
topic |
Hypertension Exercise Rats Calcium Signaling Intracellular Calcium Sensing Proteins |
description |
Abstract Background: Regulation of intracellular calcium (Ca2+) in cardiomyocytes is altered by hypertension; and aerobic exercise brings benefits to hypertensive individuals. Objective: To verify the effects of aerobic exercise training on contractility and intracellular calcium (Ca2+) transients of cardiomyocytes and on the expression of microRNA 214 (miR-214) in the left ventricle of spontaneously hypertensive rats (SHR). Methods: SHR and normotensive Wistar rats of 16 weeks were divided into 4 groups -sedentary hypertensive (SH); trained hypertensive (TH); sedentary normotensive (SN); and trained normotensive (TN). Animals of the TH and TN groups were subjected to treadmill running program, 5 days/week, 1 hour/day at 60-70% of maximum running velocity for 8 weeks. We adopted a p ≤ 0.05 as significance level for all comparisons. Results: Exercise training reduced systolic arterial pressure in hypertensive rats. In normotensive rats, exercise training reduced the time to 50% cell relaxation and the time to peak contraction and increased the time to 50% decay of the intracellular Ca2+ transients. In SHR, exercise increased the amplitude and reduced the time to 50% decay of Ca2+ transients. Exercise training increased the expression of miR-214 in hypertensive rats only. Conclusion: The aerobic training applied in this study increased the availability of intracellular Ca2+ and accelerated the sequestration of these ions in left ventricular myocytes of hypertensive rats, despite increased expression of miR-214 and maintenance of cell contractility. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2018001400172 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2018001400172 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/abc.20180113 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Cardiologia - SBC |
publisher.none.fl_str_mv |
Sociedade Brasileira de Cardiologia - SBC |
dc.source.none.fl_str_mv |
Arquivos Brasileiros de Cardiologia v.111 n.2 2018 reponame:Arquivos Brasileiros de Cardiologia (Online) instname:Sociedade Brasileira de Cardiologia (SBC) instacron:SBC |
instname_str |
Sociedade Brasileira de Cardiologia (SBC) |
instacron_str |
SBC |
institution |
SBC |
reponame_str |
Arquivos Brasileiros de Cardiologia (Online) |
collection |
Arquivos Brasileiros de Cardiologia (Online) |
repository.name.fl_str_mv |
Arquivos Brasileiros de Cardiologia (Online) - Sociedade Brasileira de Cardiologia (SBC) |
repository.mail.fl_str_mv |
||arquivos@cardiol.br |
_version_ |
1752126568715517952 |