Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats

Detalhes bibliográficos
Autor(a) principal: Rodrigues,Joel Alves
Data de Publicação: 2018
Outros Autores: Prímola-Gomes,Thales Nicolau, Soares,Leôncio Lopes, Leal,Tiago Ferreira, Nóbrega,Clara, Pedrosa,Danillo Laviola, Rezende,Leonardo Mateus Teixeira, Oliveira,Edilamar Menezes de, Natali,Antonio Jose
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivos Brasileiros de Cardiologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2018001400172
Resumo: Abstract Background: Regulation of intracellular calcium (Ca2+) in cardiomyocytes is altered by hypertension; and aerobic exercise brings benefits to hypertensive individuals. Objective: To verify the effects of aerobic exercise training on contractility and intracellular calcium (Ca2+) transients of cardiomyocytes and on the expression of microRNA 214 (miR-214) in the left ventricle of spontaneously hypertensive rats (SHR). Methods: SHR and normotensive Wistar rats of 16 weeks were divided into 4 groups -sedentary hypertensive (SH); trained hypertensive (TH); sedentary normotensive (SN); and trained normotensive (TN). Animals of the TH and TN groups were subjected to treadmill running program, 5 days/week, 1 hour/day at 60-70% of maximum running velocity for 8 weeks. We adopted a p ≤ 0.05 as significance level for all comparisons. Results: Exercise training reduced systolic arterial pressure in hypertensive rats. In normotensive rats, exercise training reduced the time to 50% cell relaxation and the time to peak contraction and increased the time to 50% decay of the intracellular Ca2+ transients. In SHR, exercise increased the amplitude and reduced the time to 50% decay of Ca2+ transients. Exercise training increased the expression of miR-214 in hypertensive rats only. Conclusion: The aerobic training applied in this study increased the availability of intracellular Ca2+ and accelerated the sequestration of these ions in left ventricular myocytes of hypertensive rats, despite increased expression of miR-214 and maintenance of cell contractility.
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spelling Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive RatsHypertensionExerciseRatsCalcium SignalingIntracellular CalciumSensing ProteinsAbstract Background: Regulation of intracellular calcium (Ca2+) in cardiomyocytes is altered by hypertension; and aerobic exercise brings benefits to hypertensive individuals. Objective: To verify the effects of aerobic exercise training on contractility and intracellular calcium (Ca2+) transients of cardiomyocytes and on the expression of microRNA 214 (miR-214) in the left ventricle of spontaneously hypertensive rats (SHR). Methods: SHR and normotensive Wistar rats of 16 weeks were divided into 4 groups -sedentary hypertensive (SH); trained hypertensive (TH); sedentary normotensive (SN); and trained normotensive (TN). Animals of the TH and TN groups were subjected to treadmill running program, 5 days/week, 1 hour/day at 60-70% of maximum running velocity for 8 weeks. We adopted a p ≤ 0.05 as significance level for all comparisons. Results: Exercise training reduced systolic arterial pressure in hypertensive rats. In normotensive rats, exercise training reduced the time to 50% cell relaxation and the time to peak contraction and increased the time to 50% decay of the intracellular Ca2+ transients. In SHR, exercise increased the amplitude and reduced the time to 50% decay of Ca2+ transients. Exercise training increased the expression of miR-214 in hypertensive rats only. Conclusion: The aerobic training applied in this study increased the availability of intracellular Ca2+ and accelerated the sequestration of these ions in left ventricular myocytes of hypertensive rats, despite increased expression of miR-214 and maintenance of cell contractility.Sociedade Brasileira de Cardiologia - SBC2018-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2018001400172Arquivos Brasileiros de Cardiologia v.111 n.2 2018reponame:Arquivos Brasileiros de Cardiologia (Online)instname:Sociedade Brasileira de Cardiologia (SBC)instacron:SBC10.5935/abc.20180113info:eu-repo/semantics/openAccessRodrigues,Joel AlvesPrímola-Gomes,Thales NicolauSoares,Leôncio LopesLeal,Tiago FerreiraNóbrega,ClaraPedrosa,Danillo LaviolaRezende,Leonardo Mateus TeixeiraOliveira,Edilamar Menezes deNatali,Antonio Joseeng2019-01-17T00:00:00Zoai:scielo:S0066-782X2018001400172Revistahttp://www.arquivosonline.com.br/https://old.scielo.br/oai/scielo-oai.php||arquivos@cardiol.br1678-41700066-782Xopendoar:2019-01-17T00:00Arquivos Brasileiros de Cardiologia (Online) - Sociedade Brasileira de Cardiologia (SBC)false
dc.title.none.fl_str_mv Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats
title Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats
spellingShingle Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats
Rodrigues,Joel Alves
Hypertension
Exercise
Rats
Calcium Signaling
Intracellular Calcium
Sensing Proteins
title_short Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats
title_full Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats
title_fullStr Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats
title_full_unstemmed Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats
title_sort Physical Exercise and Regulation of Intracellular Calcium in Cardiomyocytes of Hypertensive Rats
author Rodrigues,Joel Alves
author_facet Rodrigues,Joel Alves
Prímola-Gomes,Thales Nicolau
Soares,Leôncio Lopes
Leal,Tiago Ferreira
Nóbrega,Clara
Pedrosa,Danillo Laviola
Rezende,Leonardo Mateus Teixeira
Oliveira,Edilamar Menezes de
Natali,Antonio Jose
author_role author
author2 Prímola-Gomes,Thales Nicolau
Soares,Leôncio Lopes
Leal,Tiago Ferreira
Nóbrega,Clara
Pedrosa,Danillo Laviola
Rezende,Leonardo Mateus Teixeira
Oliveira,Edilamar Menezes de
Natali,Antonio Jose
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rodrigues,Joel Alves
Prímola-Gomes,Thales Nicolau
Soares,Leôncio Lopes
Leal,Tiago Ferreira
Nóbrega,Clara
Pedrosa,Danillo Laviola
Rezende,Leonardo Mateus Teixeira
Oliveira,Edilamar Menezes de
Natali,Antonio Jose
dc.subject.por.fl_str_mv Hypertension
Exercise
Rats
Calcium Signaling
Intracellular Calcium
Sensing Proteins
topic Hypertension
Exercise
Rats
Calcium Signaling
Intracellular Calcium
Sensing Proteins
description Abstract Background: Regulation of intracellular calcium (Ca2+) in cardiomyocytes is altered by hypertension; and aerobic exercise brings benefits to hypertensive individuals. Objective: To verify the effects of aerobic exercise training on contractility and intracellular calcium (Ca2+) transients of cardiomyocytes and on the expression of microRNA 214 (miR-214) in the left ventricle of spontaneously hypertensive rats (SHR). Methods: SHR and normotensive Wistar rats of 16 weeks were divided into 4 groups -sedentary hypertensive (SH); trained hypertensive (TH); sedentary normotensive (SN); and trained normotensive (TN). Animals of the TH and TN groups were subjected to treadmill running program, 5 days/week, 1 hour/day at 60-70% of maximum running velocity for 8 weeks. We adopted a p ≤ 0.05 as significance level for all comparisons. Results: Exercise training reduced systolic arterial pressure in hypertensive rats. In normotensive rats, exercise training reduced the time to 50% cell relaxation and the time to peak contraction and increased the time to 50% decay of the intracellular Ca2+ transients. In SHR, exercise increased the amplitude and reduced the time to 50% decay of Ca2+ transients. Exercise training increased the expression of miR-214 in hypertensive rats only. Conclusion: The aerobic training applied in this study increased the availability of intracellular Ca2+ and accelerated the sequestration of these ions in left ventricular myocytes of hypertensive rats, despite increased expression of miR-214 and maintenance of cell contractility.
publishDate 2018
dc.date.none.fl_str_mv 2018-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2018001400172
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2018001400172
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.5935/abc.20180113
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Cardiologia - SBC
publisher.none.fl_str_mv Sociedade Brasileira de Cardiologia - SBC
dc.source.none.fl_str_mv Arquivos Brasileiros de Cardiologia v.111 n.2 2018
reponame:Arquivos Brasileiros de Cardiologia (Online)
instname:Sociedade Brasileira de Cardiologia (SBC)
instacron:SBC
instname_str Sociedade Brasileira de Cardiologia (SBC)
instacron_str SBC
institution SBC
reponame_str Arquivos Brasileiros de Cardiologia (Online)
collection Arquivos Brasileiros de Cardiologia (Online)
repository.name.fl_str_mv Arquivos Brasileiros de Cardiologia (Online) - Sociedade Brasileira de Cardiologia (SBC)
repository.mail.fl_str_mv ||arquivos@cardiol.br
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