Skills to Perform Vessel Eversion in Mouse Cervical Cardiac Transplantation with Cuff Technique
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Cardiovascular Surgery (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382021000300318 |
Resumo: | Abstract Introduction: The mouse heterotopic cardiac transplant model has been extensively used to explore transplant immunity. Although the cuff technique facilitates the operation, the procedure remains difficult, and vessel eversion is the most difficult step. Cuff movement and everted vessel wall slippage are the main adverse factors in vessel eversion. Traditional strategies to prevent these factors focus on cuff fixation, while more steps or surgical instruments would be required. Methods: According to the reported protocols and our experience, the vessel eversion skills were modified and used for transplantation. Cardiac grafts from C57BL/6(H-2b) or BALB/c(H-2d) mice were transplanted into C57BL/6(H-2b) mice. The operating times of recent 90 operations, which were divided into 9 groups according to their sequence, were summarized and analyzed. Results: The mouse cervical cardiac transplantation was successfully performed by using the modified vessel eversion skills. The cuff movement, which is the most important adverse factor to prevent vessel eversion, was effectively prevented. In the recent 90 operations, the total operating time was 47.3±7.9 min and the success rate was 98%. Conclusions: The modified surgical skills simplify the vessel eversion in mouse cervical cardiac transplantation with cuff technique, characterized by less cuff movement, fewer steps, and surgical instruments. Using these surgical skills, the transplant can be performed in a short time. |
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Brazilian Journal of Cardiovascular Surgery (Online) |
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Skills to Perform Vessel Eversion in Mouse Cervical Cardiac Transplantation with Cuff TechniqueHeart TransplantationTissue DonorsHeartVascular FactorSurgical InstrumentsAnimalsAbstract Introduction: The mouse heterotopic cardiac transplant model has been extensively used to explore transplant immunity. Although the cuff technique facilitates the operation, the procedure remains difficult, and vessel eversion is the most difficult step. Cuff movement and everted vessel wall slippage are the main adverse factors in vessel eversion. Traditional strategies to prevent these factors focus on cuff fixation, while more steps or surgical instruments would be required. Methods: According to the reported protocols and our experience, the vessel eversion skills were modified and used for transplantation. Cardiac grafts from C57BL/6(H-2b) or BALB/c(H-2d) mice were transplanted into C57BL/6(H-2b) mice. The operating times of recent 90 operations, which were divided into 9 groups according to their sequence, were summarized and analyzed. Results: The mouse cervical cardiac transplantation was successfully performed by using the modified vessel eversion skills. The cuff movement, which is the most important adverse factor to prevent vessel eversion, was effectively prevented. In the recent 90 operations, the total operating time was 47.3±7.9 min and the success rate was 98%. Conclusions: The modified surgical skills simplify the vessel eversion in mouse cervical cardiac transplantation with cuff technique, characterized by less cuff movement, fewer steps, and surgical instruments. Using these surgical skills, the transplant can be performed in a short time.Sociedade Brasileira de Cirurgia Cardiovascular2021-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382021000300318Brazilian Journal of Cardiovascular Surgery v.36 n.3 2021reponame:Brazilian Journal of Cardiovascular Surgery (Online)instname:Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV)instacron:SBCCV10.21470/1678-9741-2020-0125info:eu-repo/semantics/openAccessTan,LiangXie,XubiaoXu,YananTian,QianchuanZhang,QianLan,GongbinWang,HongxiaZhao,YongPeng,Longkaieng2021-08-09T00:00:00Zoai:scielo:S0102-76382021000300318Revistahttp://www.rbccv.org.br/https://old.scielo.br/oai/scielo-oai.php||rosangela.monteiro@incor.usp.br|| domingo@braile.com.br|| brandau@braile.com.br1678-97410102-7638opendoar:2021-08-09T00:00Brazilian Journal of Cardiovascular Surgery (Online) - Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV)false |
dc.title.none.fl_str_mv |
Skills to Perform Vessel Eversion in Mouse Cervical Cardiac Transplantation with Cuff Technique |
title |
Skills to Perform Vessel Eversion in Mouse Cervical Cardiac Transplantation with Cuff Technique |
spellingShingle |
Skills to Perform Vessel Eversion in Mouse Cervical Cardiac Transplantation with Cuff Technique Tan,Liang Heart Transplantation Tissue Donors Heart Vascular Factor Surgical Instruments Animals |
title_short |
Skills to Perform Vessel Eversion in Mouse Cervical Cardiac Transplantation with Cuff Technique |
title_full |
Skills to Perform Vessel Eversion in Mouse Cervical Cardiac Transplantation with Cuff Technique |
title_fullStr |
Skills to Perform Vessel Eversion in Mouse Cervical Cardiac Transplantation with Cuff Technique |
title_full_unstemmed |
Skills to Perform Vessel Eversion in Mouse Cervical Cardiac Transplantation with Cuff Technique |
title_sort |
Skills to Perform Vessel Eversion in Mouse Cervical Cardiac Transplantation with Cuff Technique |
author |
Tan,Liang |
author_facet |
Tan,Liang Xie,Xubiao Xu,Yanan Tian,Qianchuan Zhang,Qian Lan,Gongbin Wang,Hongxia Zhao,Yong Peng,Longkai |
author_role |
author |
author2 |
Xie,Xubiao Xu,Yanan Tian,Qianchuan Zhang,Qian Lan,Gongbin Wang,Hongxia Zhao,Yong Peng,Longkai |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Tan,Liang Xie,Xubiao Xu,Yanan Tian,Qianchuan Zhang,Qian Lan,Gongbin Wang,Hongxia Zhao,Yong Peng,Longkai |
dc.subject.por.fl_str_mv |
Heart Transplantation Tissue Donors Heart Vascular Factor Surgical Instruments Animals |
topic |
Heart Transplantation Tissue Donors Heart Vascular Factor Surgical Instruments Animals |
description |
Abstract Introduction: The mouse heterotopic cardiac transplant model has been extensively used to explore transplant immunity. Although the cuff technique facilitates the operation, the procedure remains difficult, and vessel eversion is the most difficult step. Cuff movement and everted vessel wall slippage are the main adverse factors in vessel eversion. Traditional strategies to prevent these factors focus on cuff fixation, while more steps or surgical instruments would be required. Methods: According to the reported protocols and our experience, the vessel eversion skills were modified and used for transplantation. Cardiac grafts from C57BL/6(H-2b) or BALB/c(H-2d) mice were transplanted into C57BL/6(H-2b) mice. The operating times of recent 90 operations, which were divided into 9 groups according to their sequence, were summarized and analyzed. Results: The mouse cervical cardiac transplantation was successfully performed by using the modified vessel eversion skills. The cuff movement, which is the most important adverse factor to prevent vessel eversion, was effectively prevented. In the recent 90 operations, the total operating time was 47.3±7.9 min and the success rate was 98%. Conclusions: The modified surgical skills simplify the vessel eversion in mouse cervical cardiac transplantation with cuff technique, characterized by less cuff movement, fewer steps, and surgical instruments. Using these surgical skills, the transplant can be performed in a short time. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382021000300318 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-76382021000300318 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.21470/1678-9741-2020-0125 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Cirurgia Cardiovascular |
publisher.none.fl_str_mv |
Sociedade Brasileira de Cirurgia Cardiovascular |
dc.source.none.fl_str_mv |
Brazilian Journal of Cardiovascular Surgery v.36 n.3 2021 reponame:Brazilian Journal of Cardiovascular Surgery (Online) instname:Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV) instacron:SBCCV |
instname_str |
Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV) |
instacron_str |
SBCCV |
institution |
SBCCV |
reponame_str |
Brazilian Journal of Cardiovascular Surgery (Online) |
collection |
Brazilian Journal of Cardiovascular Surgery (Online) |
repository.name.fl_str_mv |
Brazilian Journal of Cardiovascular Surgery (Online) - Sociedade Brasileira de Cirurgia Cardiovascular (SBCCV) |
repository.mail.fl_str_mv |
||rosangela.monteiro@incor.usp.br|| domingo@braile.com.br|| brandau@braile.com.br |
_version_ |
1752126602655825920 |