THE ACTION OF SERICIN PROTEIN ON INITIAL NERVE REPAIR, ASSOCIATED OR NOT WITH SWIMMING IN WISTAR RATS

Detalhes bibliográficos
Autor(a) principal: Debastiani,Jean Carlos
Data de Publicação: 2019
Outros Autores: Santana Junior,André, Tavares,Alana Ludemila de Freitas, Costa,Juliana Roncini Gomes da, Machado,Luiz Gustavo Vasconcelos, Kunz,Regina Inês, Ribeiro,Lucinéia de Fátima Chasko, Costa,Rose Meire, Bertolini,Gladson Ricardo Flor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Coluna/Columna
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-18512019000400262
Resumo: ABSTRACT Objective: To analyze the effects of sericin treatment, associated or not with swimming with load exercise, on initial sciatic nerve repair after compression in Wistar rats. Methods: Forty animals were divided into five groups: control, injury, injury-sericin, injury-swimming and injury-sericin-swimming. During the axonotmesis procedure, the sericin was applied to the injury-sericin and injury-sericin-swimming groups. The injury-swimming and injury-sericin-swimming groups performed the swimming with load exercise for five days, beginning on the third postoperative day (PO), and were evaluated for function, nociception and allodynia. Euthanasia was performed on the 8th PO day and fragments of the nerve were collected and prepared for quantitative and descriptive analysis in relation to the total amount of viable nerve fibers and non-viable nerve fibers, nerve fiber diameter, axon diameter and myelin sheath thickness. Results: There was no significant improvement in the sciatic functional index up to the eighth day. The Von Frey test of the surgical scar and plantar fascia indicated a reduction in pain and allodynia for the injury-swimming and injury-sericin-swimming groups. The morphological analysis presented similar characteristics in the injury-sericin, injury-swimming and injury-sericin-swimming groups, but there was a significant difference in the number of smaller non-viable nerve fibers in the injury-swimming and injury-sericin-swimming groups as compared to the others. Conclusions: Isolated sericin protein presented proinflammatory characteristics. There was improvement of allodynia and a decrease in the pain at the site of the surgical incision, possibly linked to an aquatic effect. There was no acceleration of nerve repair on the eighth day after the injury. Level of Evidence I; High quality randomized clinical trial with or without statistically significant difference, but with narrow confidence intervals.
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spelling THE ACTION OF SERICIN PROTEIN ON INITIAL NERVE REPAIR, ASSOCIATED OR NOT WITH SWIMMING IN WISTAR RATSBiocompatible materialsNerve crushSwimmingSilkABSTRACT Objective: To analyze the effects of sericin treatment, associated or not with swimming with load exercise, on initial sciatic nerve repair after compression in Wistar rats. Methods: Forty animals were divided into five groups: control, injury, injury-sericin, injury-swimming and injury-sericin-swimming. During the axonotmesis procedure, the sericin was applied to the injury-sericin and injury-sericin-swimming groups. The injury-swimming and injury-sericin-swimming groups performed the swimming with load exercise for five days, beginning on the third postoperative day (PO), and were evaluated for function, nociception and allodynia. Euthanasia was performed on the 8th PO day and fragments of the nerve were collected and prepared for quantitative and descriptive analysis in relation to the total amount of viable nerve fibers and non-viable nerve fibers, nerve fiber diameter, axon diameter and myelin sheath thickness. Results: There was no significant improvement in the sciatic functional index up to the eighth day. The Von Frey test of the surgical scar and plantar fascia indicated a reduction in pain and allodynia for the injury-swimming and injury-sericin-swimming groups. The morphological analysis presented similar characteristics in the injury-sericin, injury-swimming and injury-sericin-swimming groups, but there was a significant difference in the number of smaller non-viable nerve fibers in the injury-swimming and injury-sericin-swimming groups as compared to the others. Conclusions: Isolated sericin protein presented proinflammatory characteristics. There was improvement of allodynia and a decrease in the pain at the site of the surgical incision, possibly linked to an aquatic effect. There was no acceleration of nerve repair on the eighth day after the injury. Level of Evidence I; High quality randomized clinical trial with or without statistically significant difference, but with narrow confidence intervals.Sociedade Brasileira de Coluna2019-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-18512019000400262Coluna/Columna v.18 n.4 2019reponame:Coluna/Columnainstname:Sociedade Brasileira de Coluna (SBCO)instacron:SBCO10.1590/s1808-185120191804181924info:eu-repo/semantics/openAccessDebastiani,Jean CarlosSantana Junior,AndréTavares,Alana Ludemila de FreitasCosta,Juliana Roncini Gomes daMachado,Luiz Gustavo VasconcelosKunz,Regina InêsRibeiro,Lucinéia de Fátima ChaskoCosta,Rose MeireBertolini,Gladson Ricardo Floreng2019-11-12T00:00:00Zoai:scielo:S1808-18512019000400262Revistahttps://www.revistacoluna.org/ONGhttps://old.scielo.br/oai/scielo-oai.phpcoluna.columna@uol.com.br||revistacoluna@uol.com.br2177-014X1808-1851opendoar:2019-11-12T00:00Coluna/Columna - Sociedade Brasileira de Coluna (SBCO)false
dc.title.none.fl_str_mv THE ACTION OF SERICIN PROTEIN ON INITIAL NERVE REPAIR, ASSOCIATED OR NOT WITH SWIMMING IN WISTAR RATS
title THE ACTION OF SERICIN PROTEIN ON INITIAL NERVE REPAIR, ASSOCIATED OR NOT WITH SWIMMING IN WISTAR RATS
spellingShingle THE ACTION OF SERICIN PROTEIN ON INITIAL NERVE REPAIR, ASSOCIATED OR NOT WITH SWIMMING IN WISTAR RATS
Debastiani,Jean Carlos
Biocompatible materials
Nerve crush
Swimming
Silk
title_short THE ACTION OF SERICIN PROTEIN ON INITIAL NERVE REPAIR, ASSOCIATED OR NOT WITH SWIMMING IN WISTAR RATS
title_full THE ACTION OF SERICIN PROTEIN ON INITIAL NERVE REPAIR, ASSOCIATED OR NOT WITH SWIMMING IN WISTAR RATS
title_fullStr THE ACTION OF SERICIN PROTEIN ON INITIAL NERVE REPAIR, ASSOCIATED OR NOT WITH SWIMMING IN WISTAR RATS
title_full_unstemmed THE ACTION OF SERICIN PROTEIN ON INITIAL NERVE REPAIR, ASSOCIATED OR NOT WITH SWIMMING IN WISTAR RATS
title_sort THE ACTION OF SERICIN PROTEIN ON INITIAL NERVE REPAIR, ASSOCIATED OR NOT WITH SWIMMING IN WISTAR RATS
author Debastiani,Jean Carlos
author_facet Debastiani,Jean Carlos
Santana Junior,André
Tavares,Alana Ludemila de Freitas
Costa,Juliana Roncini Gomes da
Machado,Luiz Gustavo Vasconcelos
Kunz,Regina Inês
Ribeiro,Lucinéia de Fátima Chasko
Costa,Rose Meire
Bertolini,Gladson Ricardo Flor
author_role author
author2 Santana Junior,André
Tavares,Alana Ludemila de Freitas
Costa,Juliana Roncini Gomes da
Machado,Luiz Gustavo Vasconcelos
Kunz,Regina Inês
Ribeiro,Lucinéia de Fátima Chasko
Costa,Rose Meire
Bertolini,Gladson Ricardo Flor
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Debastiani,Jean Carlos
Santana Junior,André
Tavares,Alana Ludemila de Freitas
Costa,Juliana Roncini Gomes da
Machado,Luiz Gustavo Vasconcelos
Kunz,Regina Inês
Ribeiro,Lucinéia de Fátima Chasko
Costa,Rose Meire
Bertolini,Gladson Ricardo Flor
dc.subject.por.fl_str_mv Biocompatible materials
Nerve crush
Swimming
Silk
topic Biocompatible materials
Nerve crush
Swimming
Silk
description ABSTRACT Objective: To analyze the effects of sericin treatment, associated or not with swimming with load exercise, on initial sciatic nerve repair after compression in Wistar rats. Methods: Forty animals were divided into five groups: control, injury, injury-sericin, injury-swimming and injury-sericin-swimming. During the axonotmesis procedure, the sericin was applied to the injury-sericin and injury-sericin-swimming groups. The injury-swimming and injury-sericin-swimming groups performed the swimming with load exercise for five days, beginning on the third postoperative day (PO), and were evaluated for function, nociception and allodynia. Euthanasia was performed on the 8th PO day and fragments of the nerve were collected and prepared for quantitative and descriptive analysis in relation to the total amount of viable nerve fibers and non-viable nerve fibers, nerve fiber diameter, axon diameter and myelin sheath thickness. Results: There was no significant improvement in the sciatic functional index up to the eighth day. The Von Frey test of the surgical scar and plantar fascia indicated a reduction in pain and allodynia for the injury-swimming and injury-sericin-swimming groups. The morphological analysis presented similar characteristics in the injury-sericin, injury-swimming and injury-sericin-swimming groups, but there was a significant difference in the number of smaller non-viable nerve fibers in the injury-swimming and injury-sericin-swimming groups as compared to the others. Conclusions: Isolated sericin protein presented proinflammatory characteristics. There was improvement of allodynia and a decrease in the pain at the site of the surgical incision, possibly linked to an aquatic effect. There was no acceleration of nerve repair on the eighth day after the injury. Level of Evidence I; High quality randomized clinical trial with or without statistically significant difference, but with narrow confidence intervals.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-18512019000400262
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1808-18512019000400262
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s1808-185120191804181924
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Coluna
publisher.none.fl_str_mv Sociedade Brasileira de Coluna
dc.source.none.fl_str_mv Coluna/Columna v.18 n.4 2019
reponame:Coluna/Columna
instname:Sociedade Brasileira de Coluna (SBCO)
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collection Coluna/Columna
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