Inflammatory bowel and oxidative stress changes in an experimental model of portal hypertension: action of N-acetylcysteine
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of Coloproctology (Rio de Janeiro. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2237-93632016000400231 |
Resumo: | ABSTRACT Introduction: Portal hypertension (PH) is characterized by vasodilatation in the portal system and the bowel is one of the severely affected organs. N-acetylcysteine (NAC) is a molecule with important properties and widely used in clinical practice. Objective: To evaluate NAC action in the bowel of animals submitted to the animal model of partial portal vein ligation (PPVL). Methods: 18 male Wistar rats were divided into three experimental groups (n = 6): sham-operated (SO), PPVL, and PPVL + NAC. On the 8th day after surgery, N-acetylcysteine (10 mg/kg, ip) was administered daily for 7 days. On the 15th day the animals’ bowel was collected for oxidative stress analysis, immunohistochemistry and Western blot. We evaluated the expression of NF-KB and TNF-α by immunohistochemistry and of iNOS by Western blot. Lipid peroxidation was assessed by TBARS technique, and the activities of antioxidant enzymes superoxide dismutase (SOD) and glutation peroxidase (GPx) were checked. Results: We observed an increased expression of NF-KB and TNF-α in PPVL group, and an increased iNOS expression assessed by Western blot. NAC reduced the expression of all proteins evaluated. We also observed an increase in oxidative stress in the bowel of mice PPVL group compared to controls (SO), and NAC was effective in reducing these values in PPVL + NAC group. Also, a reduction in the activity of SOD and GPx enzymes was observed in the diseased group, and NAC was able to restore the activity of the enzymes assessed. Conclusion: We suggest the anti-inflammatory and antioxidant action of NAC in the bowel of animals submitted to PPVL model. |
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Journal of Coloproctology (Rio de Janeiro. Online) |
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Inflammatory bowel and oxidative stress changes in an experimental model of portal hypertension: action of N-acetylcysteineN-acetylcysteinePortal hypertensionIntestineOxidative stressInflammationABSTRACT Introduction: Portal hypertension (PH) is characterized by vasodilatation in the portal system and the bowel is one of the severely affected organs. N-acetylcysteine (NAC) is a molecule with important properties and widely used in clinical practice. Objective: To evaluate NAC action in the bowel of animals submitted to the animal model of partial portal vein ligation (PPVL). Methods: 18 male Wistar rats were divided into three experimental groups (n = 6): sham-operated (SO), PPVL, and PPVL + NAC. On the 8th day after surgery, N-acetylcysteine (10 mg/kg, ip) was administered daily for 7 days. On the 15th day the animals’ bowel was collected for oxidative stress analysis, immunohistochemistry and Western blot. We evaluated the expression of NF-KB and TNF-α by immunohistochemistry and of iNOS by Western blot. Lipid peroxidation was assessed by TBARS technique, and the activities of antioxidant enzymes superoxide dismutase (SOD) and glutation peroxidase (GPx) were checked. Results: We observed an increased expression of NF-KB and TNF-α in PPVL group, and an increased iNOS expression assessed by Western blot. NAC reduced the expression of all proteins evaluated. We also observed an increase in oxidative stress in the bowel of mice PPVL group compared to controls (SO), and NAC was effective in reducing these values in PPVL + NAC group. Also, a reduction in the activity of SOD and GPx enzymes was observed in the diseased group, and NAC was able to restore the activity of the enzymes assessed. Conclusion: We suggest the anti-inflammatory and antioxidant action of NAC in the bowel of animals submitted to PPVL model.Sociedade Brasileira de Coloproctologia2016-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2237-93632016000400231Journal of Coloproctology (Rio de Janeiro) v.36 n.4 2016reponame:Journal of Coloproctology (Rio de Janeiro. Online)instname:Sociedade Brasileira de Coloproctologia (SBCP)instacron:SBCP10.1016/j.jcol.2016.05.005info:eu-repo/semantics/openAccessLicks,FrancielliHartmann,Renata MinuzzoSchemitt,ElizângelaColares,Josieli RaskopfFillmann,Lúcio SarubbiFillmann,HenriqueMarroni,Norma Possaeng2016-12-15T00:00:00Zoai:scielo:S2237-93632016000400231Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=2237-9363&lng=pt&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||sbcp@sbcp.org.br2317-64232237-9363opendoar:2016-12-15T00:00Journal of Coloproctology (Rio de Janeiro. Online) - Sociedade Brasileira de Coloproctologia (SBCP)false |
dc.title.none.fl_str_mv |
Inflammatory bowel and oxidative stress changes in an experimental model of portal hypertension: action of N-acetylcysteine |
title |
Inflammatory bowel and oxidative stress changes in an experimental model of portal hypertension: action of N-acetylcysteine |
spellingShingle |
Inflammatory bowel and oxidative stress changes in an experimental model of portal hypertension: action of N-acetylcysteine Licks,Francielli N-acetylcysteine Portal hypertension Intestine Oxidative stress Inflammation |
title_short |
Inflammatory bowel and oxidative stress changes in an experimental model of portal hypertension: action of N-acetylcysteine |
title_full |
Inflammatory bowel and oxidative stress changes in an experimental model of portal hypertension: action of N-acetylcysteine |
title_fullStr |
Inflammatory bowel and oxidative stress changes in an experimental model of portal hypertension: action of N-acetylcysteine |
title_full_unstemmed |
Inflammatory bowel and oxidative stress changes in an experimental model of portal hypertension: action of N-acetylcysteine |
title_sort |
Inflammatory bowel and oxidative stress changes in an experimental model of portal hypertension: action of N-acetylcysteine |
author |
Licks,Francielli |
author_facet |
Licks,Francielli Hartmann,Renata Minuzzo Schemitt,Elizângela Colares,Josieli Raskopf Fillmann,Lúcio Sarubbi Fillmann,Henrique Marroni,Norma Possa |
author_role |
author |
author2 |
Hartmann,Renata Minuzzo Schemitt,Elizângela Colares,Josieli Raskopf Fillmann,Lúcio Sarubbi Fillmann,Henrique Marroni,Norma Possa |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Licks,Francielli Hartmann,Renata Minuzzo Schemitt,Elizângela Colares,Josieli Raskopf Fillmann,Lúcio Sarubbi Fillmann,Henrique Marroni,Norma Possa |
dc.subject.por.fl_str_mv |
N-acetylcysteine Portal hypertension Intestine Oxidative stress Inflammation |
topic |
N-acetylcysteine Portal hypertension Intestine Oxidative stress Inflammation |
description |
ABSTRACT Introduction: Portal hypertension (PH) is characterized by vasodilatation in the portal system and the bowel is one of the severely affected organs. N-acetylcysteine (NAC) is a molecule with important properties and widely used in clinical practice. Objective: To evaluate NAC action in the bowel of animals submitted to the animal model of partial portal vein ligation (PPVL). Methods: 18 male Wistar rats were divided into three experimental groups (n = 6): sham-operated (SO), PPVL, and PPVL + NAC. On the 8th day after surgery, N-acetylcysteine (10 mg/kg, ip) was administered daily for 7 days. On the 15th day the animals’ bowel was collected for oxidative stress analysis, immunohistochemistry and Western blot. We evaluated the expression of NF-KB and TNF-α by immunohistochemistry and of iNOS by Western blot. Lipid peroxidation was assessed by TBARS technique, and the activities of antioxidant enzymes superoxide dismutase (SOD) and glutation peroxidase (GPx) were checked. Results: We observed an increased expression of NF-KB and TNF-α in PPVL group, and an increased iNOS expression assessed by Western blot. NAC reduced the expression of all proteins evaluated. We also observed an increase in oxidative stress in the bowel of mice PPVL group compared to controls (SO), and NAC was effective in reducing these values in PPVL + NAC group. Also, a reduction in the activity of SOD and GPx enzymes was observed in the diseased group, and NAC was able to restore the activity of the enzymes assessed. Conclusion: We suggest the anti-inflammatory and antioxidant action of NAC in the bowel of animals submitted to PPVL model. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2237-93632016000400231 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2237-93632016000400231 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.jcol.2016.05.005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Coloproctologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Coloproctologia |
dc.source.none.fl_str_mv |
Journal of Coloproctology (Rio de Janeiro) v.36 n.4 2016 reponame:Journal of Coloproctology (Rio de Janeiro. Online) instname:Sociedade Brasileira de Coloproctologia (SBCP) instacron:SBCP |
instname_str |
Sociedade Brasileira de Coloproctologia (SBCP) |
instacron_str |
SBCP |
institution |
SBCP |
reponame_str |
Journal of Coloproctology (Rio de Janeiro. Online) |
collection |
Journal of Coloproctology (Rio de Janeiro. Online) |
repository.name.fl_str_mv |
Journal of Coloproctology (Rio de Janeiro. Online) - Sociedade Brasileira de Coloproctologia (SBCP) |
repository.mail.fl_str_mv |
||sbcp@sbcp.org.br |
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1752126477881573376 |