Role of preoperative retrograde enema on inflammatory and healing parameters in colonic anastomosis: experimental study in dogs
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of Coloproctology (Rio de Janeiro. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2237-93632014000400193 |
Resumo: | Objective:The purpose of this experimental study was to compare the inflammatory and wound healing response of dogs submitted to colonic anastomosis with and without preoperative retrograde enema.Methods:The study included two groups of 31 female dogs (Canis familiaris). G-I (control): no preoperative bowel preparation; G-II (study): preoperative retrograde enema using a 10% glycerin solution. All the animals were submitted to laparotomy and colotomy at 20 cm from the anal verge, followed by closure with a running extramucosal single-layer suture (Prolene® 000). The animals were then anesthetized and euthanized on the 7th (n = 10) or 21st (n= 20) postoperative day (POD) to remove the anastomosed colon segment for histological and immunohistochemical analysis evaluating the parameters: anastomotic edema, vasoproliferation, abdominal adhesions, type I and III collagen, nitric oxide and myeloperoxidase. The observed differences were analyzed with the Mann–Whitney test for nonparametric data and Student's t test for unpaired samples and parametric data.Results:One animal from G-I and one from G-II died on POD 7 and POD 10 due to anastomotic complications and sepsis, respectively The groups did not differ significantly with regard to inflammatory and healing parameters, although the levels of mature collagen were significantly lower in the animals submitted to preoperative bowel preparation.Conclusion:It has been shown that both procedures are safe to be used, however, the group with bowel preparation showed a lower amount of mature collagen in the immediate postoperative period and may be constituted a preventive factor for surgical complications for this type of surgical procedure, although no evidence in this study could be determined. |
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Journal of Coloproctology (Rio de Janeiro. Online) |
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Role of preoperative retrograde enema on inflammatory and healing parameters in colonic anastomosis: experimental study in dogsEnemaWound healingInflammationCollagenObjective:The purpose of this experimental study was to compare the inflammatory and wound healing response of dogs submitted to colonic anastomosis with and without preoperative retrograde enema.Methods:The study included two groups of 31 female dogs (Canis familiaris). G-I (control): no preoperative bowel preparation; G-II (study): preoperative retrograde enema using a 10% glycerin solution. All the animals were submitted to laparotomy and colotomy at 20 cm from the anal verge, followed by closure with a running extramucosal single-layer suture (Prolene® 000). The animals were then anesthetized and euthanized on the 7th (n = 10) or 21st (n= 20) postoperative day (POD) to remove the anastomosed colon segment for histological and immunohistochemical analysis evaluating the parameters: anastomotic edema, vasoproliferation, abdominal adhesions, type I and III collagen, nitric oxide and myeloperoxidase. The observed differences were analyzed with the Mann–Whitney test for nonparametric data and Student's t test for unpaired samples and parametric data.Results:One animal from G-I and one from G-II died on POD 7 and POD 10 due to anastomotic complications and sepsis, respectively The groups did not differ significantly with regard to inflammatory and healing parameters, although the levels of mature collagen were significantly lower in the animals submitted to preoperative bowel preparation.Conclusion:It has been shown that both procedures are safe to be used, however, the group with bowel preparation showed a lower amount of mature collagen in the immediate postoperative period and may be constituted a preventive factor for surgical complications for this type of surgical procedure, although no evidence in this study could be determined.Sociedade Brasileira de Coloproctologia2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2237-93632014000400193Journal of Coloproctology (Rio de Janeiro) v.34 n.4 2014reponame:Journal of Coloproctology (Rio de Janeiro. Online)instname:Sociedade Brasileira de Coloproctologia (SBCP)instacron:SBCP10.1016/j.jcol.2014.08.007info:eu-repo/semantics/openAccessBezerra,Carlos Renato SalesRegadas,Francisco Sérgio PinheiroFigueiredo,Welligton RibeiroNogueira,Miguel Augusto ArcoverdeSousa,Péricles Cerqueira deLeite,Claudio Manuel Gonçalves da SilvaSilva,Kaline Fortes eMartins Filho,Erbert PortelaDiasLeite,José Albertoeng2015-09-04T00:00:00Zoai:scielo:S2237-93632014000400193Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=2237-9363&lng=pt&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||sbcp@sbcp.org.br2317-64232237-9363opendoar:2015-09-04T00:00Journal of Coloproctology (Rio de Janeiro. Online) - Sociedade Brasileira de Coloproctologia (SBCP)false |
dc.title.none.fl_str_mv |
Role of preoperative retrograde enema on inflammatory and healing parameters in colonic anastomosis: experimental study in dogs |
title |
Role of preoperative retrograde enema on inflammatory and healing parameters in colonic anastomosis: experimental study in dogs |
spellingShingle |
Role of preoperative retrograde enema on inflammatory and healing parameters in colonic anastomosis: experimental study in dogs Bezerra,Carlos Renato Sales Enema Wound healing Inflammation Collagen |
title_short |
Role of preoperative retrograde enema on inflammatory and healing parameters in colonic anastomosis: experimental study in dogs |
title_full |
Role of preoperative retrograde enema on inflammatory and healing parameters in colonic anastomosis: experimental study in dogs |
title_fullStr |
Role of preoperative retrograde enema on inflammatory and healing parameters in colonic anastomosis: experimental study in dogs |
title_full_unstemmed |
Role of preoperative retrograde enema on inflammatory and healing parameters in colonic anastomosis: experimental study in dogs |
title_sort |
Role of preoperative retrograde enema on inflammatory and healing parameters in colonic anastomosis: experimental study in dogs |
author |
Bezerra,Carlos Renato Sales |
author_facet |
Bezerra,Carlos Renato Sales Regadas,Francisco Sérgio Pinheiro Figueiredo,Welligton Ribeiro Nogueira,Miguel Augusto Arcoverde Sousa,Péricles Cerqueira de Leite,Claudio Manuel Gonçalves da Silva Silva,Kaline Fortes e Martins Filho,Erbert Portela DiasLeite,José Alberto |
author_role |
author |
author2 |
Regadas,Francisco Sérgio Pinheiro Figueiredo,Welligton Ribeiro Nogueira,Miguel Augusto Arcoverde Sousa,Péricles Cerqueira de Leite,Claudio Manuel Gonçalves da Silva Silva,Kaline Fortes e Martins Filho,Erbert Portela DiasLeite,José Alberto |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Bezerra,Carlos Renato Sales Regadas,Francisco Sérgio Pinheiro Figueiredo,Welligton Ribeiro Nogueira,Miguel Augusto Arcoverde Sousa,Péricles Cerqueira de Leite,Claudio Manuel Gonçalves da Silva Silva,Kaline Fortes e Martins Filho,Erbert Portela DiasLeite,José Alberto |
dc.subject.por.fl_str_mv |
Enema Wound healing Inflammation Collagen |
topic |
Enema Wound healing Inflammation Collagen |
description |
Objective:The purpose of this experimental study was to compare the inflammatory and wound healing response of dogs submitted to colonic anastomosis with and without preoperative retrograde enema.Methods:The study included two groups of 31 female dogs (Canis familiaris). G-I (control): no preoperative bowel preparation; G-II (study): preoperative retrograde enema using a 10% glycerin solution. All the animals were submitted to laparotomy and colotomy at 20 cm from the anal verge, followed by closure with a running extramucosal single-layer suture (Prolene® 000). The animals were then anesthetized and euthanized on the 7th (n = 10) or 21st (n= 20) postoperative day (POD) to remove the anastomosed colon segment for histological and immunohistochemical analysis evaluating the parameters: anastomotic edema, vasoproliferation, abdominal adhesions, type I and III collagen, nitric oxide and myeloperoxidase. The observed differences were analyzed with the Mann–Whitney test for nonparametric data and Student's t test for unpaired samples and parametric data.Results:One animal from G-I and one from G-II died on POD 7 and POD 10 due to anastomotic complications and sepsis, respectively The groups did not differ significantly with regard to inflammatory and healing parameters, although the levels of mature collagen were significantly lower in the animals submitted to preoperative bowel preparation.Conclusion:It has been shown that both procedures are safe to be used, however, the group with bowel preparation showed a lower amount of mature collagen in the immediate postoperative period and may be constituted a preventive factor for surgical complications for this type of surgical procedure, although no evidence in this study could be determined. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2237-93632014000400193 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2237-93632014000400193 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.jcol.2014.08.007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Coloproctologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Coloproctologia |
dc.source.none.fl_str_mv |
Journal of Coloproctology (Rio de Janeiro) v.34 n.4 2014 reponame:Journal of Coloproctology (Rio de Janeiro. Online) instname:Sociedade Brasileira de Coloproctologia (SBCP) instacron:SBCP |
instname_str |
Sociedade Brasileira de Coloproctologia (SBCP) |
instacron_str |
SBCP |
institution |
SBCP |
reponame_str |
Journal of Coloproctology (Rio de Janeiro. Online) |
collection |
Journal of Coloproctology (Rio de Janeiro. Online) |
repository.name.fl_str_mv |
Journal of Coloproctology (Rio de Janeiro. Online) - Sociedade Brasileira de Coloproctologia (SBCP) |
repository.mail.fl_str_mv |
||sbcp@sbcp.org.br |
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1752126477475774464 |