Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts

Detalhes bibliográficos
Autor(a) principal: Isaac,César
Data de Publicação: 2011
Outros Autores: Paggiaro,André Oliveira, Aldunate,Johnny Leandro Conduta Borda, Herson,Marisa Roma, Altran,Silvana Cereijido, Mônica Beatriz,Mathor, Ferreira,Marcus Castro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Brasileira de Cirurgia Plástica (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-51752011000300007
Resumo: BACKGROUND: The possible participation of keratinocytes in wound remodeling has been widely studied. This study investigated the impact of keratinocytes in wound contraction. METHODS: Murine type I collagen gels populated by human fibroblasts and seeded with human keratinocytes on the surface to form a dermo-epidermal equivalent were used as the study group. Collagen gels populated by only fibroblasts were used as the control group. The criteria for the preparation and storage of gels were similar for both groups. RESULTS: An evident and statistically significant increase in gel contraction was observed in samples populated by keratinocytes compared to the control group. CONCLUSIONS: These results suggest that keratinocytes not only modulate fibroblast proliferation but also play an active role in wound contraction per se. Further research on the mechanisms involved in the communication pathways between cells and between cells and the matrix shall be assessed from the perspective of keratinocyte participation in wound healing and pathologic scarring.
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spelling Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblastsKeratinocytesWound healingCell culture techniquesFibroblastsBACKGROUND: The possible participation of keratinocytes in wound remodeling has been widely studied. This study investigated the impact of keratinocytes in wound contraction. METHODS: Murine type I collagen gels populated by human fibroblasts and seeded with human keratinocytes on the surface to form a dermo-epidermal equivalent were used as the study group. Collagen gels populated by only fibroblasts were used as the control group. The criteria for the preparation and storage of gels were similar for both groups. RESULTS: An evident and statistically significant increase in gel contraction was observed in samples populated by keratinocytes compared to the control group. CONCLUSIONS: These results suggest that keratinocytes not only modulate fibroblast proliferation but also play an active role in wound contraction per se. Further research on the mechanisms involved in the communication pathways between cells and between cells and the matrix shall be assessed from the perspective of keratinocyte participation in wound healing and pathologic scarring.Sociedade Brasileira de Cirurgia Plástica2011-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-51752011000300007Revista Brasileira de Cirurgia Plástica v.26 n.3 2011reponame:Revista Brasileira de Cirurgia Plástica (Online)instname:Sociedade Brasileira de Cirurgia Plástica (SBCP)instacron:SBCP10.1590/S1983-51752011000300007info:eu-repo/semantics/openAccessIsaac,CésarPaggiaro,André OliveiraAldunate,Johnny Leandro Conduta BordaHerson,Marisa RomaAltran,Silvana CereijidoMônica Beatriz,MathorFerreira,Marcus Castroeng2011-12-09T00:00:00Zoai:scielo:S1983-51752011000300007Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=1983-5175&lng=pt&nrm=isoONGhttps://old.scielo.br/oai/scielo-oai.php||rbcp@cirurgiaplastica.org.br2177-12351983-5175opendoar:2011-12-09T00:00Revista Brasileira de Cirurgia Plástica (Online) - Sociedade Brasileira de Cirurgia Plástica (SBCP)false
dc.title.none.fl_str_mv Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts
title Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts
spellingShingle Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts
Isaac,César
Keratinocytes
Wound healing
Cell culture techniques
Fibroblasts
title_short Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts
title_full Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts
title_fullStr Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts
title_full_unstemmed Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts
title_sort Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts
author Isaac,César
author_facet Isaac,César
Paggiaro,André Oliveira
Aldunate,Johnny Leandro Conduta Borda
Herson,Marisa Roma
Altran,Silvana Cereijido
Mônica Beatriz,Mathor
Ferreira,Marcus Castro
author_role author
author2 Paggiaro,André Oliveira
Aldunate,Johnny Leandro Conduta Borda
Herson,Marisa Roma
Altran,Silvana Cereijido
Mônica Beatriz,Mathor
Ferreira,Marcus Castro
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Isaac,César
Paggiaro,André Oliveira
Aldunate,Johnny Leandro Conduta Borda
Herson,Marisa Roma
Altran,Silvana Cereijido
Mônica Beatriz,Mathor
Ferreira,Marcus Castro
dc.subject.por.fl_str_mv Keratinocytes
Wound healing
Cell culture techniques
Fibroblasts
topic Keratinocytes
Wound healing
Cell culture techniques
Fibroblasts
description BACKGROUND: The possible participation of keratinocytes in wound remodeling has been widely studied. This study investigated the impact of keratinocytes in wound contraction. METHODS: Murine type I collagen gels populated by human fibroblasts and seeded with human keratinocytes on the surface to form a dermo-epidermal equivalent were used as the study group. Collagen gels populated by only fibroblasts were used as the control group. The criteria for the preparation and storage of gels were similar for both groups. RESULTS: An evident and statistically significant increase in gel contraction was observed in samples populated by keratinocytes compared to the control group. CONCLUSIONS: These results suggest that keratinocytes not only modulate fibroblast proliferation but also play an active role in wound contraction per se. Further research on the mechanisms involved in the communication pathways between cells and between cells and the matrix shall be assessed from the perspective of keratinocyte participation in wound healing and pathologic scarring.
publishDate 2011
dc.date.none.fl_str_mv 2011-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-51752011000300007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-51752011000300007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1983-51752011000300007
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Cirurgia Plástica
publisher.none.fl_str_mv Sociedade Brasileira de Cirurgia Plástica
dc.source.none.fl_str_mv Revista Brasileira de Cirurgia Plástica v.26 n.3 2011
reponame:Revista Brasileira de Cirurgia Plástica (Online)
instname:Sociedade Brasileira de Cirurgia Plástica (SBCP)
instacron:SBCP
instname_str Sociedade Brasileira de Cirurgia Plástica (SBCP)
instacron_str SBCP
institution SBCP
reponame_str Revista Brasileira de Cirurgia Plástica (Online)
collection Revista Brasileira de Cirurgia Plástica (Online)
repository.name.fl_str_mv Revista Brasileira de Cirurgia Plástica (Online) - Sociedade Brasileira de Cirurgia Plástica (SBCP)
repository.mail.fl_str_mv ||rbcp@cirurgiaplastica.org.br
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