Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Cirurgia Plástica (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-51752011000300007 |
Resumo: | BACKGROUND: The possible participation of keratinocytes in wound remodeling has been widely studied. This study investigated the impact of keratinocytes in wound contraction. METHODS: Murine type I collagen gels populated by human fibroblasts and seeded with human keratinocytes on the surface to form a dermo-epidermal equivalent were used as the study group. Collagen gels populated by only fibroblasts were used as the control group. The criteria for the preparation and storage of gels were similar for both groups. RESULTS: An evident and statistically significant increase in gel contraction was observed in samples populated by keratinocytes compared to the control group. CONCLUSIONS: These results suggest that keratinocytes not only modulate fibroblast proliferation but also play an active role in wound contraction per se. Further research on the mechanisms involved in the communication pathways between cells and between cells and the matrix shall be assessed from the perspective of keratinocyte participation in wound healing and pathologic scarring. |
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Revista Brasileira de Cirurgia Plástica (Online) |
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|
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Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblastsKeratinocytesWound healingCell culture techniquesFibroblastsBACKGROUND: The possible participation of keratinocytes in wound remodeling has been widely studied. This study investigated the impact of keratinocytes in wound contraction. METHODS: Murine type I collagen gels populated by human fibroblasts and seeded with human keratinocytes on the surface to form a dermo-epidermal equivalent were used as the study group. Collagen gels populated by only fibroblasts were used as the control group. The criteria for the preparation and storage of gels were similar for both groups. RESULTS: An evident and statistically significant increase in gel contraction was observed in samples populated by keratinocytes compared to the control group. CONCLUSIONS: These results suggest that keratinocytes not only modulate fibroblast proliferation but also play an active role in wound contraction per se. Further research on the mechanisms involved in the communication pathways between cells and between cells and the matrix shall be assessed from the perspective of keratinocyte participation in wound healing and pathologic scarring.Sociedade Brasileira de Cirurgia Plástica2011-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-51752011000300007Revista Brasileira de Cirurgia Plástica v.26 n.3 2011reponame:Revista Brasileira de Cirurgia Plástica (Online)instname:Sociedade Brasileira de Cirurgia Plástica (SBCP)instacron:SBCP10.1590/S1983-51752011000300007info:eu-repo/semantics/openAccessIsaac,CésarPaggiaro,André OliveiraAldunate,Johnny Leandro Conduta BordaHerson,Marisa RomaAltran,Silvana CereijidoMônica Beatriz,MathorFerreira,Marcus Castroeng2011-12-09T00:00:00Zoai:scielo:S1983-51752011000300007Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=1983-5175&lng=pt&nrm=isoONGhttps://old.scielo.br/oai/scielo-oai.php||rbcp@cirurgiaplastica.org.br2177-12351983-5175opendoar:2011-12-09T00:00Revista Brasileira de Cirurgia Plástica (Online) - Sociedade Brasileira de Cirurgia Plástica (SBCP)false |
dc.title.none.fl_str_mv |
Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts |
title |
Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts |
spellingShingle |
Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts Isaac,César Keratinocytes Wound healing Cell culture techniques Fibroblasts |
title_short |
Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts |
title_full |
Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts |
title_fullStr |
Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts |
title_full_unstemmed |
Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts |
title_sort |
Role of keratinocytes in wound contraction: an impact assessment using a model of collagen matrix populated with fibroblasts |
author |
Isaac,César |
author_facet |
Isaac,César Paggiaro,André Oliveira Aldunate,Johnny Leandro Conduta Borda Herson,Marisa Roma Altran,Silvana Cereijido Mônica Beatriz,Mathor Ferreira,Marcus Castro |
author_role |
author |
author2 |
Paggiaro,André Oliveira Aldunate,Johnny Leandro Conduta Borda Herson,Marisa Roma Altran,Silvana Cereijido Mônica Beatriz,Mathor Ferreira,Marcus Castro |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Isaac,César Paggiaro,André Oliveira Aldunate,Johnny Leandro Conduta Borda Herson,Marisa Roma Altran,Silvana Cereijido Mônica Beatriz,Mathor Ferreira,Marcus Castro |
dc.subject.por.fl_str_mv |
Keratinocytes Wound healing Cell culture techniques Fibroblasts |
topic |
Keratinocytes Wound healing Cell culture techniques Fibroblasts |
description |
BACKGROUND: The possible participation of keratinocytes in wound remodeling has been widely studied. This study investigated the impact of keratinocytes in wound contraction. METHODS: Murine type I collagen gels populated by human fibroblasts and seeded with human keratinocytes on the surface to form a dermo-epidermal equivalent were used as the study group. Collagen gels populated by only fibroblasts were used as the control group. The criteria for the preparation and storage of gels were similar for both groups. RESULTS: An evident and statistically significant increase in gel contraction was observed in samples populated by keratinocytes compared to the control group. CONCLUSIONS: These results suggest that keratinocytes not only modulate fibroblast proliferation but also play an active role in wound contraction per se. Further research on the mechanisms involved in the communication pathways between cells and between cells and the matrix shall be assessed from the perspective of keratinocyte participation in wound healing and pathologic scarring. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-51752011000300007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1983-51752011000300007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1983-51752011000300007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Cirurgia Plástica |
publisher.none.fl_str_mv |
Sociedade Brasileira de Cirurgia Plástica |
dc.source.none.fl_str_mv |
Revista Brasileira de Cirurgia Plástica v.26 n.3 2011 reponame:Revista Brasileira de Cirurgia Plástica (Online) instname:Sociedade Brasileira de Cirurgia Plástica (SBCP) instacron:SBCP |
instname_str |
Sociedade Brasileira de Cirurgia Plástica (SBCP) |
instacron_str |
SBCP |
institution |
SBCP |
reponame_str |
Revista Brasileira de Cirurgia Plástica (Online) |
collection |
Revista Brasileira de Cirurgia Plástica (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Cirurgia Plástica (Online) - Sociedade Brasileira de Cirurgia Plástica (SBCP) |
repository.mail.fl_str_mv |
||rbcp@cirurgiaplastica.org.br |
_version_ |
1754821114562871296 |