Lupeol, a pentacyclic triterpene, promotes migration, wound closure, and contractile effect in vitro: Possible involvement of PI3K/Akt and p38/ERK/MAPK pathways

Detalhes bibliográficos
Autor(a) principal: Beserra, Fernando Pereira [UNESP]
Data de Publicação: 2018
Outros Autores: Xue, Meilang, De Azevedo Maia, Gabriela Lemos, Rozza, Ariane Leite [UNESP], Pellizzon, Cláudia Helena [UNESP], Jackson, Christopher John
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/molecules23112819
http://hdl.handle.net/11449/189853
Resumo: Skin wound healing is a dynamic and complex process involving several mediators at the cellular and molecular levels. Lupeol, a phytoconstituent belonging to the triterpenes class, is found in several fruit plants and medicinal plants that have been the object of study in the treatment of various diseases, including skin wounds. Various medicinal properties of lupeol have been reported in the literature, including anti-inflammatory, antioxidant, anti-diabetic, and anti-mutagenic effects. We investigated the effects of lupeol (0.1, 1, 10, and 20 µg/mL) on in vitro wound healing assays and signaling mechanisms in human neonatal foreskin keratinocytes and fibroblasts. Results showed that, at high concentrations, Lupeol reduced cell proliferation of both keratinocytes and fibroblasts, but increased in vitro wound healing in keratinocytes and promoted the contraction of dermal fibroblasts in the collagen gel matrix. This triterpene positively regulated matrix metalloproteinase (MMP)-2 and inhibited the NF-κB expression in keratinocytes, suggesting an anti-inflammatory effect. Lupeol also modulated the expression of keratin 16 according to the concentration tested. Additionally, in keratinocytes, lupeol treatment resulted in the activation of Akt, p38, and Tie-2, which are signaling proteins involved in cell proliferation and migration, angiogenesis, and tissue repair. These findings suggest that lupeol has therapeutic potential for accelerating wound healing.
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spelling Lupeol, a pentacyclic triterpene, promotes migration, wound closure, and contractile effect in vitro: Possible involvement of PI3K/Akt and p38/ERK/MAPK pathwaysCell migrationFibroblastsKeratinocytesLupeolWound healingSkin wound healing is a dynamic and complex process involving several mediators at the cellular and molecular levels. Lupeol, a phytoconstituent belonging to the triterpenes class, is found in several fruit plants and medicinal plants that have been the object of study in the treatment of various diseases, including skin wounds. Various medicinal properties of lupeol have been reported in the literature, including anti-inflammatory, antioxidant, anti-diabetic, and anti-mutagenic effects. We investigated the effects of lupeol (0.1, 1, 10, and 20 µg/mL) on in vitro wound healing assays and signaling mechanisms in human neonatal foreskin keratinocytes and fibroblasts. Results showed that, at high concentrations, Lupeol reduced cell proliferation of both keratinocytes and fibroblasts, but increased in vitro wound healing in keratinocytes and promoted the contraction of dermal fibroblasts in the collagen gel matrix. This triterpene positively regulated matrix metalloproteinase (MMP)-2 and inhibited the NF-κB expression in keratinocytes, suggesting an anti-inflammatory effect. Lupeol also modulated the expression of keratin 16 according to the concentration tested. Additionally, in keratinocytes, lupeol treatment resulted in the activation of Akt, p38, and Tie-2, which are signaling proteins involved in cell proliferation and migration, angiogenesis, and tissue repair. These findings suggest that lupeol has therapeutic potential for accelerating wound healing.Department of Morphology Institute of Biosciences São Paulo State University (UNESP)Sutton Research Laboratory Kolling Institute of Medical Research University of Sydney Royal North Shore HospitalDepartment of Pharmacy Federal University of São Francisco Valley (UNIVASF)Department of Morphology Institute of Biosciences São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Royal North Shore HospitalFederal University of São Francisco Valley (UNIVASF)Beserra, Fernando Pereira [UNESP]Xue, MeilangDe Azevedo Maia, Gabriela LemosRozza, Ariane Leite [UNESP]Pellizzon, Cláudia Helena [UNESP]Jackson, Christopher John2019-10-06T16:54:16Z2019-10-06T16:54:16Z2018-10-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/molecules23112819Molecules, v. 23, n. 11, 2018.1420-3049http://hdl.handle.net/11449/18985310.3390/molecules231128192-s2.0-8505583027400193937798010690000-0002-4494-4180Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMoleculesinfo:eu-repo/semantics/openAccess2021-10-27T11:38:32Zoai:repositorio.unesp.br:11449/189853Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:01:21.615394Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Lupeol, a pentacyclic triterpene, promotes migration, wound closure, and contractile effect in vitro: Possible involvement of PI3K/Akt and p38/ERK/MAPK pathways
title Lupeol, a pentacyclic triterpene, promotes migration, wound closure, and contractile effect in vitro: Possible involvement of PI3K/Akt and p38/ERK/MAPK pathways
spellingShingle Lupeol, a pentacyclic triterpene, promotes migration, wound closure, and contractile effect in vitro: Possible involvement of PI3K/Akt and p38/ERK/MAPK pathways
Beserra, Fernando Pereira [UNESP]
Cell migration
Fibroblasts
Keratinocytes
Lupeol
Wound healing
title_short Lupeol, a pentacyclic triterpene, promotes migration, wound closure, and contractile effect in vitro: Possible involvement of PI3K/Akt and p38/ERK/MAPK pathways
title_full Lupeol, a pentacyclic triterpene, promotes migration, wound closure, and contractile effect in vitro: Possible involvement of PI3K/Akt and p38/ERK/MAPK pathways
title_fullStr Lupeol, a pentacyclic triterpene, promotes migration, wound closure, and contractile effect in vitro: Possible involvement of PI3K/Akt and p38/ERK/MAPK pathways
title_full_unstemmed Lupeol, a pentacyclic triterpene, promotes migration, wound closure, and contractile effect in vitro: Possible involvement of PI3K/Akt and p38/ERK/MAPK pathways
title_sort Lupeol, a pentacyclic triterpene, promotes migration, wound closure, and contractile effect in vitro: Possible involvement of PI3K/Akt and p38/ERK/MAPK pathways
author Beserra, Fernando Pereira [UNESP]
author_facet Beserra, Fernando Pereira [UNESP]
Xue, Meilang
De Azevedo Maia, Gabriela Lemos
Rozza, Ariane Leite [UNESP]
Pellizzon, Cláudia Helena [UNESP]
Jackson, Christopher John
author_role author
author2 Xue, Meilang
De Azevedo Maia, Gabriela Lemos
Rozza, Ariane Leite [UNESP]
Pellizzon, Cláudia Helena [UNESP]
Jackson, Christopher John
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Royal North Shore Hospital
Federal University of São Francisco Valley (UNIVASF)
dc.contributor.author.fl_str_mv Beserra, Fernando Pereira [UNESP]
Xue, Meilang
De Azevedo Maia, Gabriela Lemos
Rozza, Ariane Leite [UNESP]
Pellizzon, Cláudia Helena [UNESP]
Jackson, Christopher John
dc.subject.por.fl_str_mv Cell migration
Fibroblasts
Keratinocytes
Lupeol
Wound healing
topic Cell migration
Fibroblasts
Keratinocytes
Lupeol
Wound healing
description Skin wound healing is a dynamic and complex process involving several mediators at the cellular and molecular levels. Lupeol, a phytoconstituent belonging to the triterpenes class, is found in several fruit plants and medicinal plants that have been the object of study in the treatment of various diseases, including skin wounds. Various medicinal properties of lupeol have been reported in the literature, including anti-inflammatory, antioxidant, anti-diabetic, and anti-mutagenic effects. We investigated the effects of lupeol (0.1, 1, 10, and 20 µg/mL) on in vitro wound healing assays and signaling mechanisms in human neonatal foreskin keratinocytes and fibroblasts. Results showed that, at high concentrations, Lupeol reduced cell proliferation of both keratinocytes and fibroblasts, but increased in vitro wound healing in keratinocytes and promoted the contraction of dermal fibroblasts in the collagen gel matrix. This triterpene positively regulated matrix metalloproteinase (MMP)-2 and inhibited the NF-κB expression in keratinocytes, suggesting an anti-inflammatory effect. Lupeol also modulated the expression of keratin 16 according to the concentration tested. Additionally, in keratinocytes, lupeol treatment resulted in the activation of Akt, p38, and Tie-2, which are signaling proteins involved in cell proliferation and migration, angiogenesis, and tissue repair. These findings suggest that lupeol has therapeutic potential for accelerating wound healing.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-30
2019-10-06T16:54:16Z
2019-10-06T16:54:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/molecules23112819
Molecules, v. 23, n. 11, 2018.
1420-3049
http://hdl.handle.net/11449/189853
10.3390/molecules23112819
2-s2.0-85055830274
0019393779801069
0000-0002-4494-4180
url http://dx.doi.org/10.3390/molecules23112819
http://hdl.handle.net/11449/189853
identifier_str_mv Molecules, v. 23, n. 11, 2018.
1420-3049
10.3390/molecules23112819
2-s2.0-85055830274
0019393779801069
0000-0002-4494-4180
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Molecules
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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