Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Planta daninha (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582012000300025 |
Resumo: | Eleusine indica (goosegrass) is a diploid grass weed which has developed resistance to ACCase inhibitors during the last ten years due to the intensive and frequent use of sethoxydim to control grass weeds in soybean crops in Brazil. Plant dose-response assays confirmed the resistant behaviour of one biotype obtaining high resistance factor values: 143 (fenoxaprop), 126 (haloxyfop), 84 (sethoxydim) to 58 (fluazifop). ACCase in vitro assays indicated a target site resistance as the main cause of reduced susceptibility to ACCase inhibitors. PCR-generated fragments of the ACCase CT domain of the resistant and sensitive reference biotype were sequenced and compared. A point mutation was detected within the triplet of aspartate at the amino acid position 2078 (referred to EMBL accession no. AJ310767) and resulted in the triplet of glycine. These results constitute the first report on a target site mutation for a Brazilian herbicide resistant grass weed. |
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Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutationgoosegrasscross-resistanceenzymePCRherbicideEleusine indica (goosegrass) is a diploid grass weed which has developed resistance to ACCase inhibitors during the last ten years due to the intensive and frequent use of sethoxydim to control grass weeds in soybean crops in Brazil. Plant dose-response assays confirmed the resistant behaviour of one biotype obtaining high resistance factor values: 143 (fenoxaprop), 126 (haloxyfop), 84 (sethoxydim) to 58 (fluazifop). ACCase in vitro assays indicated a target site resistance as the main cause of reduced susceptibility to ACCase inhibitors. PCR-generated fragments of the ACCase CT domain of the resistant and sensitive reference biotype were sequenced and compared. A point mutation was detected within the triplet of aspartate at the amino acid position 2078 (referred to EMBL accession no. AJ310767) and resulted in the triplet of glycine. These results constitute the first report on a target site mutation for a Brazilian herbicide resistant grass weed.Sociedade Brasileira da Ciência das Plantas Daninhas 2012-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582012000300025Planta Daninha v.30 n.3 2012reponame:Planta daninha (Online)instname:Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)instacron:SBCPD10.1590/S0100-83582012000300025info:eu-repo/semantics/openAccessOsuna,M.D.Goulart,I.C.G.R.Vidal,R.A.Kalsing,A.Ruiz Santaella,J.P.De Prado,R.eng2012-08-16T00:00:00Zoai:scielo:S0100-83582012000300025Revistahttp://revistas.cpd.ufv.br/pdaninhaweb/https://old.scielo.br/oai/scielo-oai.php||rpdaninha@gmail.com1806-96810100-8358opendoar:2012-08-16T00:00Planta daninha (Online) - Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)false |
dc.title.none.fl_str_mv |
Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation |
title |
Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation |
spellingShingle |
Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation Osuna,M.D. goosegrass cross-resistance enzyme PCR herbicide |
title_short |
Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation |
title_full |
Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation |
title_fullStr |
Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation |
title_full_unstemmed |
Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation |
title_sort |
Resistance to ACCase inhibitors in Eleusine indica from Brazil involves a target site mutation |
author |
Osuna,M.D. |
author_facet |
Osuna,M.D. Goulart,I.C.G.R. Vidal,R.A. Kalsing,A. Ruiz Santaella,J.P. De Prado,R. |
author_role |
author |
author2 |
Goulart,I.C.G.R. Vidal,R.A. Kalsing,A. Ruiz Santaella,J.P. De Prado,R. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Osuna,M.D. Goulart,I.C.G.R. Vidal,R.A. Kalsing,A. Ruiz Santaella,J.P. De Prado,R. |
dc.subject.por.fl_str_mv |
goosegrass cross-resistance enzyme PCR herbicide |
topic |
goosegrass cross-resistance enzyme PCR herbicide |
description |
Eleusine indica (goosegrass) is a diploid grass weed which has developed resistance to ACCase inhibitors during the last ten years due to the intensive and frequent use of sethoxydim to control grass weeds in soybean crops in Brazil. Plant dose-response assays confirmed the resistant behaviour of one biotype obtaining high resistance factor values: 143 (fenoxaprop), 126 (haloxyfop), 84 (sethoxydim) to 58 (fluazifop). ACCase in vitro assays indicated a target site resistance as the main cause of reduced susceptibility to ACCase inhibitors. PCR-generated fragments of the ACCase CT domain of the resistant and sensitive reference biotype were sequenced and compared. A point mutation was detected within the triplet of aspartate at the amino acid position 2078 (referred to EMBL accession no. AJ310767) and resulted in the triplet of glycine. These results constitute the first report on a target site mutation for a Brazilian herbicide resistant grass weed. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582012000300025 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582012000300025 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-83582012000300025 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira da Ciência das Plantas Daninhas |
publisher.none.fl_str_mv |
Sociedade Brasileira da Ciência das Plantas Daninhas |
dc.source.none.fl_str_mv |
Planta Daninha v.30 n.3 2012 reponame:Planta daninha (Online) instname:Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD) instacron:SBCPD |
instname_str |
Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD) |
instacron_str |
SBCPD |
institution |
SBCPD |
reponame_str |
Planta daninha (Online) |
collection |
Planta daninha (Online) |
repository.name.fl_str_mv |
Planta daninha (Online) - Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD) |
repository.mail.fl_str_mv |
||rpdaninha@gmail.com |
_version_ |
1752126493505355776 |