Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici

Detalhes bibliográficos
Autor(a) principal: NAQVI,S.F.
Data de Publicação: 2019
Outros Autores: JAVAID,A., QURESHI,M.Z.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Planta daninha (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100165
Resumo: ABSTRACT: The present study was performed to evaluate antifungal activity and GC-MS analysis of leaf extract of Chenopodium mural against Fusarium oxysporum f. sp. lycopersici (FOL), a highly problematic soil-borne pathogen of tomato. Dried leaves of C. murale were extracted with methanol for 2 weeks and after evaporating the solvent on a rotary evaporator, antifungal bioassay was carried out against FOL. All concentrations (1 to 5%) significantly reduced FOL biomass by 14-45%. The remaining methanolic extract was fractioned with n-hexane, chloroform and ethyl acetate and all these fractions were assayed for their antifungal potential. A 200 mg mL-1 concentration of various sub-fractions reduced fungal biomass significantly by 94-98% over control. All the sub-fractions were subjected to GC-MS analysis that revealed presence of 32 compounds in n-hexane, 2 compounds in chloroform and 13 compounds in ethyl acetate sub-fraction. The predominant compounds in n-hexane sub-fraction were hexadecanoic acid, methyl ester (14.64%), methyl linolenate (16.61%) and g-sitosterol (13.53%). In chloroform sub-fraction, bis (2-ethylhexyl) phthalate (92.31% and in ethyl-acetate sub-fraction, ethyl butyrate (19.57%), dihexyl phthalate (11.19%) and dioctyl phthalate (12.16%) were present in higher concentration.
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spelling Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersiciFusarium wiltGC-MS analysistomatoABSTRACT: The present study was performed to evaluate antifungal activity and GC-MS analysis of leaf extract of Chenopodium mural against Fusarium oxysporum f. sp. lycopersici (FOL), a highly problematic soil-borne pathogen of tomato. Dried leaves of C. murale were extracted with methanol for 2 weeks and after evaporating the solvent on a rotary evaporator, antifungal bioassay was carried out against FOL. All concentrations (1 to 5%) significantly reduced FOL biomass by 14-45%. The remaining methanolic extract was fractioned with n-hexane, chloroform and ethyl acetate and all these fractions were assayed for their antifungal potential. A 200 mg mL-1 concentration of various sub-fractions reduced fungal biomass significantly by 94-98% over control. All the sub-fractions were subjected to GC-MS analysis that revealed presence of 32 compounds in n-hexane, 2 compounds in chloroform and 13 compounds in ethyl acetate sub-fraction. The predominant compounds in n-hexane sub-fraction were hexadecanoic acid, methyl ester (14.64%), methyl linolenate (16.61%) and g-sitosterol (13.53%). In chloroform sub-fraction, bis (2-ethylhexyl) phthalate (92.31% and in ethyl-acetate sub-fraction, ethyl butyrate (19.57%), dihexyl phthalate (11.19%) and dioctyl phthalate (12.16%) were present in higher concentration.Sociedade Brasileira da Ciência das Plantas Daninhas 2019-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100165Planta Daninha v.37 2019reponame:Planta daninha (Online)instname:Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)instacron:SBCPD10.1590/s0100-83582019370100139info:eu-repo/semantics/openAccessNAQVI,S.F.JAVAID,A.QURESHI,M.Z.eng2019-11-27T00:00:00Zoai:scielo:S0100-83582019000100165Revistahttp://revistas.cpd.ufv.br/pdaninhaweb/https://old.scielo.br/oai/scielo-oai.php||rpdaninha@gmail.com1806-96810100-8358opendoar:2019-11-27T00:00Planta daninha (Online) - Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)false
dc.title.none.fl_str_mv Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici
title Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici
spellingShingle Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici
NAQVI,S.F.
Fusarium wilt
GC-MS analysis
tomato
title_short Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici
title_full Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici
title_fullStr Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici
title_full_unstemmed Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici
title_sort Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici
author NAQVI,S.F.
author_facet NAQVI,S.F.
JAVAID,A.
QURESHI,M.Z.
author_role author
author2 JAVAID,A.
QURESHI,M.Z.
author2_role author
author
dc.contributor.author.fl_str_mv NAQVI,S.F.
JAVAID,A.
QURESHI,M.Z.
dc.subject.por.fl_str_mv Fusarium wilt
GC-MS analysis
tomato
topic Fusarium wilt
GC-MS analysis
tomato
description ABSTRACT: The present study was performed to evaluate antifungal activity and GC-MS analysis of leaf extract of Chenopodium mural against Fusarium oxysporum f. sp. lycopersici (FOL), a highly problematic soil-borne pathogen of tomato. Dried leaves of C. murale were extracted with methanol for 2 weeks and after evaporating the solvent on a rotary evaporator, antifungal bioassay was carried out against FOL. All concentrations (1 to 5%) significantly reduced FOL biomass by 14-45%. The remaining methanolic extract was fractioned with n-hexane, chloroform and ethyl acetate and all these fractions were assayed for their antifungal potential. A 200 mg mL-1 concentration of various sub-fractions reduced fungal biomass significantly by 94-98% over control. All the sub-fractions were subjected to GC-MS analysis that revealed presence of 32 compounds in n-hexane, 2 compounds in chloroform and 13 compounds in ethyl acetate sub-fraction. The predominant compounds in n-hexane sub-fraction were hexadecanoic acid, methyl ester (14.64%), methyl linolenate (16.61%) and g-sitosterol (13.53%). In chloroform sub-fraction, bis (2-ethylhexyl) phthalate (92.31% and in ethyl-acetate sub-fraction, ethyl butyrate (19.57%), dihexyl phthalate (11.19%) and dioctyl phthalate (12.16%) were present in higher concentration.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582019000100165
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s0100-83582019370100139
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira da Ciência das Plantas Daninhas
publisher.none.fl_str_mv Sociedade Brasileira da Ciência das Plantas Daninhas
dc.source.none.fl_str_mv Planta Daninha v.37 2019
reponame:Planta daninha (Online)
instname:Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)
instacron:SBCPD
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reponame_str Planta daninha (Online)
collection Planta daninha (Online)
repository.name.fl_str_mv Planta daninha (Online) - Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD)
repository.mail.fl_str_mv ||rpdaninha@gmail.com
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