Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necator
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Ciência do Solo (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832018000100407 |
Resumo: | ABSTRACT Preliminary results of in vitro experiments with multicontaminated soils and solid media indicated that nodulating diazotrophic bacteria of the genus Cupriavidus are promising for the remediation of contaminated environments due to their symbiosis with legumes and metal tolerance. Thus, strains of Cupriavidus spp. (LMG 19424T, UFLA 01-659, UFLA 01-663, and UFLA 02-71) were tested for their ability to tolerate and bioaccumulate cadmium (Cd), copper (Cu), and zinc (Zn) in Luria-Bertani broth. Changes in the growth pattern of Cupriavidus strains in the presence or absence of heavy metals were analyzed by scanning electron microscopy and metal allocation by transmission electron microscopy, to clarify the mechanisms of bioremediation. Highest tolerance was detected for strain UFLA 01-659 (minimum inhibitory concentration of 5, 4.95, and 14.66 mmol L−1 of Cd, Cu, and Zn, respectively). Among the removal rates of the metals tested (9.0, 4.6, and 3.2 mg L−1 of Cd, Cu, and Zn, respectively), the bacterial activity was clearly highest for Cd. The efficiency of strain UFLA 01-659 in removing the heavy metals is associated with its high biomass production and/or higher contents of heavy metals adsorbed and absorbed in the biomass. In response to the presence of heavy metals in the liquid culture medium, the bacteria produced exopolysaccharides and small and aggregated cells. However, these responses varied according to the strains and heavy metals. Regarding allocation, all heavy metals were adsorbed on the cell wall and membrane, whereas complexation was observed intracellularly and only for Cu and Zn. These results indicate the possibility of using C. necator UFLA 01-659 for remediation in areas with very high Cd, Cu, and Zn contents. |
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Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necatorheavy metalselectron microscopydiazotrophic bacteriabioremediationtolerance mechanismsABSTRACT Preliminary results of in vitro experiments with multicontaminated soils and solid media indicated that nodulating diazotrophic bacteria of the genus Cupriavidus are promising for the remediation of contaminated environments due to their symbiosis with legumes and metal tolerance. Thus, strains of Cupriavidus spp. (LMG 19424T, UFLA 01-659, UFLA 01-663, and UFLA 02-71) were tested for their ability to tolerate and bioaccumulate cadmium (Cd), copper (Cu), and zinc (Zn) in Luria-Bertani broth. Changes in the growth pattern of Cupriavidus strains in the presence or absence of heavy metals were analyzed by scanning electron microscopy and metal allocation by transmission electron microscopy, to clarify the mechanisms of bioremediation. Highest tolerance was detected for strain UFLA 01-659 (minimum inhibitory concentration of 5, 4.95, and 14.66 mmol L−1 of Cd, Cu, and Zn, respectively). Among the removal rates of the metals tested (9.0, 4.6, and 3.2 mg L−1 of Cd, Cu, and Zn, respectively), the bacterial activity was clearly highest for Cd. The efficiency of strain UFLA 01-659 in removing the heavy metals is associated with its high biomass production and/or higher contents of heavy metals adsorbed and absorbed in the biomass. In response to the presence of heavy metals in the liquid culture medium, the bacteria produced exopolysaccharides and small and aggregated cells. However, these responses varied according to the strains and heavy metals. Regarding allocation, all heavy metals were adsorbed on the cell wall and membrane, whereas complexation was observed intracellularly and only for Cu and Zn. These results indicate the possibility of using C. necator UFLA 01-659 for remediation in areas with very high Cd, Cu, and Zn contents.Sociedade Brasileira de Ciência do Solo2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832018000100407Revista Brasileira de Ciência do Solo v.42 2018reponame:Revista Brasileira de Ciência do Solo (Online)instname:Sociedade Brasileira de Ciência do Solo (SBCS)instacron:SBCS10.1590/18069657rbcs20170080info:eu-repo/semantics/openAccessVicentin,Rayssa PereiraSantos,Jessé Valentim dosLabory,Cláudia Regina GontijoCosta,Amanda Monique daMoreira,Fatima Maria de SouzaAlves,Eduardoeng2018-03-06T00:00:00Zoai:scielo:S0100-06832018000100407Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=0100-0683&lng=es&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||sbcs@ufv.br1806-96570100-0683opendoar:2018-03-06T00:00Revista Brasileira de Ciência do Solo (Online) - Sociedade Brasileira de Ciência do Solo (SBCS)false |
dc.title.none.fl_str_mv |
Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necator |
title |
Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necator |
spellingShingle |
Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necator Vicentin,Rayssa Pereira heavy metals electron microscopy diazotrophic bacteria bioremediation tolerance mechanisms |
title_short |
Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necator |
title_full |
Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necator |
title_fullStr |
Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necator |
title_full_unstemmed |
Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necator |
title_sort |
Tolerance to and Accumulation of Cadmium, Copper, and Zinc by Cupriavidus necator |
author |
Vicentin,Rayssa Pereira |
author_facet |
Vicentin,Rayssa Pereira Santos,Jessé Valentim dos Labory,Cláudia Regina Gontijo Costa,Amanda Monique da Moreira,Fatima Maria de Souza Alves,Eduardo |
author_role |
author |
author2 |
Santos,Jessé Valentim dos Labory,Cláudia Regina Gontijo Costa,Amanda Monique da Moreira,Fatima Maria de Souza Alves,Eduardo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Vicentin,Rayssa Pereira Santos,Jessé Valentim dos Labory,Cláudia Regina Gontijo Costa,Amanda Monique da Moreira,Fatima Maria de Souza Alves,Eduardo |
dc.subject.por.fl_str_mv |
heavy metals electron microscopy diazotrophic bacteria bioremediation tolerance mechanisms |
topic |
heavy metals electron microscopy diazotrophic bacteria bioremediation tolerance mechanisms |
description |
ABSTRACT Preliminary results of in vitro experiments with multicontaminated soils and solid media indicated that nodulating diazotrophic bacteria of the genus Cupriavidus are promising for the remediation of contaminated environments due to their symbiosis with legumes and metal tolerance. Thus, strains of Cupriavidus spp. (LMG 19424T, UFLA 01-659, UFLA 01-663, and UFLA 02-71) were tested for their ability to tolerate and bioaccumulate cadmium (Cd), copper (Cu), and zinc (Zn) in Luria-Bertani broth. Changes in the growth pattern of Cupriavidus strains in the presence or absence of heavy metals were analyzed by scanning electron microscopy and metal allocation by transmission electron microscopy, to clarify the mechanisms of bioremediation. Highest tolerance was detected for strain UFLA 01-659 (minimum inhibitory concentration of 5, 4.95, and 14.66 mmol L−1 of Cd, Cu, and Zn, respectively). Among the removal rates of the metals tested (9.0, 4.6, and 3.2 mg L−1 of Cd, Cu, and Zn, respectively), the bacterial activity was clearly highest for Cd. The efficiency of strain UFLA 01-659 in removing the heavy metals is associated with its high biomass production and/or higher contents of heavy metals adsorbed and absorbed in the biomass. In response to the presence of heavy metals in the liquid culture medium, the bacteria produced exopolysaccharides and small and aggregated cells. However, these responses varied according to the strains and heavy metals. Regarding allocation, all heavy metals were adsorbed on the cell wall and membrane, whereas complexation was observed intracellularly and only for Cu and Zn. These results indicate the possibility of using C. necator UFLA 01-659 for remediation in areas with very high Cd, Cu, and Zn contents. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832018000100407 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832018000100407 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/18069657rbcs20170080 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência do Solo |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência do Solo |
dc.source.none.fl_str_mv |
Revista Brasileira de Ciência do Solo v.42 2018 reponame:Revista Brasileira de Ciência do Solo (Online) instname:Sociedade Brasileira de Ciência do Solo (SBCS) instacron:SBCS |
instname_str |
Sociedade Brasileira de Ciência do Solo (SBCS) |
instacron_str |
SBCS |
institution |
SBCS |
reponame_str |
Revista Brasileira de Ciência do Solo (Online) |
collection |
Revista Brasileira de Ciência do Solo (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Ciência do Solo (Online) - Sociedade Brasileira de Ciência do Solo (SBCS) |
repository.mail.fl_str_mv |
||sbcs@ufv.br |
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1752126521774964736 |