Gene screening for fatty acid synthesis of flax based on transcriptome sequencing
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101021 |
Resumo: | Abstract To understand differential expression of genes (DEGs) based on transcriptome and transcription features of fatty acid desaturase genes (fads) in development of oil flax capsules. Three varieties of flax with high, medium and low linolenic acid (C18:3) content was investigated. The differential expressed genes (DEGs) of seed developing periods (15 d and 30 d after flowering, DAF) were compared with that of the flowering day based on RNA sequencing technology. DEGs related to fatty acid accumulation were distinguished and compared with Gene Ontolygy (GO) database and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. In the synthesis of unsaturated fatty acids and α-linolenic acid metabolism, DEGs acted in multiple processes and signaling pathways. Lus_GLEAN_10046787, Lus_GLEAN_10024616, Lus_GLEAN_10024710, Lus_GLEAN_10046516, scaffold280_unigene_193 and scaffold272_unigene_19 participated in 23, 11, 12, 12, 12, 13 processes in GO description, respectively. And they acted in fatty acid degradation (ath00071), metabolism (ath01212), biosynthesis (ath00061) and elongation (ath00062) in the KEGG signaling pathway. The scaffold96_unigene_47 was differentially expressed between FR46 and FR48 with different linolenic acid contents. The DEGs can be utilized describe the structure and transcription expression features, which contribute to the selection and utilization of flax breeding lines. |
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Food Science and Technology (Campinas) |
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Gene screening for fatty acid synthesis of flax based on transcriptome sequencingflaxgene sequencingfatty acidtranscriptome sequencingAbstract To understand differential expression of genes (DEGs) based on transcriptome and transcription features of fatty acid desaturase genes (fads) in development of oil flax capsules. Three varieties of flax with high, medium and low linolenic acid (C18:3) content was investigated. The differential expressed genes (DEGs) of seed developing periods (15 d and 30 d after flowering, DAF) were compared with that of the flowering day based on RNA sequencing technology. DEGs related to fatty acid accumulation were distinguished and compared with Gene Ontolygy (GO) database and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. In the synthesis of unsaturated fatty acids and α-linolenic acid metabolism, DEGs acted in multiple processes and signaling pathways. Lus_GLEAN_10046787, Lus_GLEAN_10024616, Lus_GLEAN_10024710, Lus_GLEAN_10046516, scaffold280_unigene_193 and scaffold272_unigene_19 participated in 23, 11, 12, 12, 12, 13 processes in GO description, respectively. And they acted in fatty acid degradation (ath00071), metabolism (ath01212), biosynthesis (ath00061) and elongation (ath00062) in the KEGG signaling pathway. The scaffold96_unigene_47 was differentially expressed between FR46 and FR48 with different linolenic acid contents. The DEGs can be utilized describe the structure and transcription expression features, which contribute to the selection and utilization of flax breeding lines.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101021Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.93721info:eu-repo/semantics/openAccessWANG,ShuyanXU,JunWANG,ZhuoLI,ZhiweiYI,LiuxiYAO,LijunWANG,Xiaeng2022-03-15T00:00:00Zoai:scielo:S0101-20612022000101021Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-03-15T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Gene screening for fatty acid synthesis of flax based on transcriptome sequencing |
title |
Gene screening for fatty acid synthesis of flax based on transcriptome sequencing |
spellingShingle |
Gene screening for fatty acid synthesis of flax based on transcriptome sequencing WANG,Shuyan flax gene sequencing fatty acid transcriptome sequencing |
title_short |
Gene screening for fatty acid synthesis of flax based on transcriptome sequencing |
title_full |
Gene screening for fatty acid synthesis of flax based on transcriptome sequencing |
title_fullStr |
Gene screening for fatty acid synthesis of flax based on transcriptome sequencing |
title_full_unstemmed |
Gene screening for fatty acid synthesis of flax based on transcriptome sequencing |
title_sort |
Gene screening for fatty acid synthesis of flax based on transcriptome sequencing |
author |
WANG,Shuyan |
author_facet |
WANG,Shuyan XU,Jun WANG,Zhuo LI,Zhiwei YI,Liuxi YAO,Lijun WANG,Xia |
author_role |
author |
author2 |
XU,Jun WANG,Zhuo LI,Zhiwei YI,Liuxi YAO,Lijun WANG,Xia |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
WANG,Shuyan XU,Jun WANG,Zhuo LI,Zhiwei YI,Liuxi YAO,Lijun WANG,Xia |
dc.subject.por.fl_str_mv |
flax gene sequencing fatty acid transcriptome sequencing |
topic |
flax gene sequencing fatty acid transcriptome sequencing |
description |
Abstract To understand differential expression of genes (DEGs) based on transcriptome and transcription features of fatty acid desaturase genes (fads) in development of oil flax capsules. Three varieties of flax with high, medium and low linolenic acid (C18:3) content was investigated. The differential expressed genes (DEGs) of seed developing periods (15 d and 30 d after flowering, DAF) were compared with that of the flowering day based on RNA sequencing technology. DEGs related to fatty acid accumulation were distinguished and compared with Gene Ontolygy (GO) database and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. In the synthesis of unsaturated fatty acids and α-linolenic acid metabolism, DEGs acted in multiple processes and signaling pathways. Lus_GLEAN_10046787, Lus_GLEAN_10024616, Lus_GLEAN_10024710, Lus_GLEAN_10046516, scaffold280_unigene_193 and scaffold272_unigene_19 participated in 23, 11, 12, 12, 12, 13 processes in GO description, respectively. And they acted in fatty acid degradation (ath00071), metabolism (ath01212), biosynthesis (ath00061) and elongation (ath00062) in the KEGG signaling pathway. The scaffold96_unigene_47 was differentially expressed between FR46 and FR48 with different linolenic acid contents. The DEGs can be utilized describe the structure and transcription expression features, which contribute to the selection and utilization of flax breeding lines. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101021 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101021 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.93721 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.42 2022 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126333794648064 |