Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000200355 |
Resumo: | Abstract The consumption of raw milk cheese has been growing worldwide with S. aureus and E. coli been the leading agents in food poisoning. The present work aims to evaluate the microbiological quality of raw milk cheeses commercialized in Brazil, regarding microbiology safety and enterotoxin gene presence. Forty-three raw milk cheeses samples from five different suppliers were analyzed. Counting and identification of S. aureus, E. coli and Salmonella spp were performed according to BAM from the FDA. Further S. aureus identification was performed by the cydB and Salmonella spp by the invA gene. S. aureus toxin genes (sea, seb, sec, see, ses, seh and sei) and E. coli gene LT, STa, Stb, stx1, stx2, eae, rmpA, wabG, mrkD, kfu, mcgA, fimH and uge were analysed. From the 43 samples analyzed, 18 presented S. aureus with two isolates positive for the tst gene, two for the sec gene, two for the seh gene and four for the sei gene. Thirty-five E. coli and seven. Salmonella spp isolates were obtained. E. coli isolates harbored sta and stx2 genes. The results revealed that raw milk cheeses sold can cause harm consumer's health and highlights the importance of adoption good hygienic-sanitary practices and consumers awareness. |
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Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogensepidemiologyvirulence genesfood safetypathogensAbstract The consumption of raw milk cheese has been growing worldwide with S. aureus and E. coli been the leading agents in food poisoning. The present work aims to evaluate the microbiological quality of raw milk cheeses commercialized in Brazil, regarding microbiology safety and enterotoxin gene presence. Forty-three raw milk cheeses samples from five different suppliers were analyzed. Counting and identification of S. aureus, E. coli and Salmonella spp were performed according to BAM from the FDA. Further S. aureus identification was performed by the cydB and Salmonella spp by the invA gene. S. aureus toxin genes (sea, seb, sec, see, ses, seh and sei) and E. coli gene LT, STa, Stb, stx1, stx2, eae, rmpA, wabG, mrkD, kfu, mcgA, fimH and uge were analysed. From the 43 samples analyzed, 18 presented S. aureus with two isolates positive for the tst gene, two for the sec gene, two for the seh gene and four for the sei gene. Thirty-five E. coli and seven. Salmonella spp isolates were obtained. E. coli isolates harbored sta and stx2 genes. The results revealed that raw milk cheeses sold can cause harm consumer's health and highlights the importance of adoption good hygienic-sanitary practices and consumers awareness.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000200355Food Science and Technology v.41 n.2 2021reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.37919info:eu-repo/semantics/openAccessCARDOZO,Marita VedovelliNESPOLO,NataliaDELFINO,Tammy ChiodaALMEIDA,Camila Chioda dePIZAURO,Lucas José LuduverioVALMORBIDA,Mylena KarolinePEREIRA,NatáliaÁVILA,Fernando Antônio deeng2021-04-05T00:00:00Zoai:scielo:S0101-20612021000200355Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2021-04-05T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens |
title |
Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens |
spellingShingle |
Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens CARDOZO,Marita Vedovelli epidemiology virulence genes food safety pathogens |
title_short |
Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens |
title_full |
Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens |
title_fullStr |
Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens |
title_full_unstemmed |
Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens |
title_sort |
Raw milk cheese as a potential infection source of pathogenic and toxigenic food born pathogens |
author |
CARDOZO,Marita Vedovelli |
author_facet |
CARDOZO,Marita Vedovelli NESPOLO,Natalia DELFINO,Tammy Chioda ALMEIDA,Camila Chioda de PIZAURO,Lucas José Luduverio VALMORBIDA,Mylena Karoline PEREIRA,Natália ÁVILA,Fernando Antônio de |
author_role |
author |
author2 |
NESPOLO,Natalia DELFINO,Tammy Chioda ALMEIDA,Camila Chioda de PIZAURO,Lucas José Luduverio VALMORBIDA,Mylena Karoline PEREIRA,Natália ÁVILA,Fernando Antônio de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
CARDOZO,Marita Vedovelli NESPOLO,Natalia DELFINO,Tammy Chioda ALMEIDA,Camila Chioda de PIZAURO,Lucas José Luduverio VALMORBIDA,Mylena Karoline PEREIRA,Natália ÁVILA,Fernando Antônio de |
dc.subject.por.fl_str_mv |
epidemiology virulence genes food safety pathogens |
topic |
epidemiology virulence genes food safety pathogens |
description |
Abstract The consumption of raw milk cheese has been growing worldwide with S. aureus and E. coli been the leading agents in food poisoning. The present work aims to evaluate the microbiological quality of raw milk cheeses commercialized in Brazil, regarding microbiology safety and enterotoxin gene presence. Forty-three raw milk cheeses samples from five different suppliers were analyzed. Counting and identification of S. aureus, E. coli and Salmonella spp were performed according to BAM from the FDA. Further S. aureus identification was performed by the cydB and Salmonella spp by the invA gene. S. aureus toxin genes (sea, seb, sec, see, ses, seh and sei) and E. coli gene LT, STa, Stb, stx1, stx2, eae, rmpA, wabG, mrkD, kfu, mcgA, fimH and uge were analysed. From the 43 samples analyzed, 18 presented S. aureus with two isolates positive for the tst gene, two for the sec gene, two for the seh gene and four for the sei gene. Thirty-five E. coli and seven. Salmonella spp isolates were obtained. E. coli isolates harbored sta and stx2 genes. The results revealed that raw milk cheeses sold can cause harm consumer's health and highlights the importance of adoption good hygienic-sanitary practices and consumers awareness. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000200355 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000200355 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.37919 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.41 n.2 2021 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126328145969152 |