Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus

Detalhes bibliográficos
Autor(a) principal: Ramella,Micheline S.
Data de Publicação: 2005
Outros Autores: Kroth,Mariela A., Tagliari,Caroline, Arisi,Ana Carolina M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Food Science and Technology (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612005000400017
Resumo: Lophius gastrophysus has important commercial value in Brazil particularly for foreign trade. In this study, we described the optimization of Random Amplified Polymorphic DNA (RAPD) protocol for identification of L. gastrophysus. Different conditions (annealing temperatures, MgCl concentrations, DNA quantity) were tested to find reproducible and adequate profiles. Amplifications performed with primers A01, ² A02 and A03 generate the best RAPD profiles when the conditions were annealing temperature of 36ºC, 25 ng of DNA quantity and 2.5 mM MgCl2. Exact identification of the species and origin of marine products is necessary and RAPD could be used as an accurate, rapid tool to expose commercial fraud.
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spelling Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysusfraudfish identificationblackfin goosefishRAPD-PCRLophius gastrophysus has important commercial value in Brazil particularly for foreign trade. In this study, we described the optimization of Random Amplified Polymorphic DNA (RAPD) protocol for identification of L. gastrophysus. Different conditions (annealing temperatures, MgCl concentrations, DNA quantity) were tested to find reproducible and adequate profiles. Amplifications performed with primers A01, ² A02 and A03 generate the best RAPD profiles when the conditions were annealing temperature of 36ºC, 25 ng of DNA quantity and 2.5 mM MgCl2. Exact identification of the species and origin of marine products is necessary and RAPD could be used as an accurate, rapid tool to expose commercial fraud.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2005-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612005000400017Food Science and Technology v.25 n.4 2005reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/S0101-20612005000400017info:eu-repo/semantics/openAccessRamella,Micheline S.Kroth,Mariela A.Tagliari,CarolineArisi,Ana Carolina M.eng2006-02-14T00:00:00Zoai:scielo:S0101-20612005000400017Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2006-02-14T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus
title Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus
spellingShingle Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus
Ramella,Micheline S.
fraud
fish identification
blackfin goosefish
RAPD-PCR
title_short Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus
title_full Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus
title_fullStr Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus
title_full_unstemmed Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus
title_sort Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus
author Ramella,Micheline S.
author_facet Ramella,Micheline S.
Kroth,Mariela A.
Tagliari,Caroline
Arisi,Ana Carolina M.
author_role author
author2 Kroth,Mariela A.
Tagliari,Caroline
Arisi,Ana Carolina M.
author2_role author
author
author
dc.contributor.author.fl_str_mv Ramella,Micheline S.
Kroth,Mariela A.
Tagliari,Caroline
Arisi,Ana Carolina M.
dc.subject.por.fl_str_mv fraud
fish identification
blackfin goosefish
RAPD-PCR
topic fraud
fish identification
blackfin goosefish
RAPD-PCR
description Lophius gastrophysus has important commercial value in Brazil particularly for foreign trade. In this study, we described the optimization of Random Amplified Polymorphic DNA (RAPD) protocol for identification of L. gastrophysus. Different conditions (annealing temperatures, MgCl concentrations, DNA quantity) were tested to find reproducible and adequate profiles. Amplifications performed with primers A01, ² A02 and A03 generate the best RAPD profiles when the conditions were annealing temperature of 36ºC, 25 ng of DNA quantity and 2.5 mM MgCl2. Exact identification of the species and origin of marine products is necessary and RAPD could be used as an accurate, rapid tool to expose commercial fraud.
publishDate 2005
dc.date.none.fl_str_mv 2005-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612005000400017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612005000400017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0101-20612005000400017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.25 n.4 2005
reponame:Food Science and Technology (Campinas)
instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron:SBCTA
instname_str Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron_str SBCTA
institution SBCTA
reponame_str Food Science and Technology (Campinas)
collection Food Science and Technology (Campinas)
repository.name.fl_str_mv Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
repository.mail.fl_str_mv ||revista@sbcta.org.br
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