Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612005000400017 |
Resumo: | Lophius gastrophysus has important commercial value in Brazil particularly for foreign trade. In this study, we described the optimization of Random Amplified Polymorphic DNA (RAPD) protocol for identification of L. gastrophysus. Different conditions (annealing temperatures, MgCl concentrations, DNA quantity) were tested to find reproducible and adequate profiles. Amplifications performed with primers A01, ² A02 and A03 generate the best RAPD profiles when the conditions were annealing temperature of 36ºC, 25 ng of DNA quantity and 2.5 mM MgCl2. Exact identification of the species and origin of marine products is necessary and RAPD could be used as an accurate, rapid tool to expose commercial fraud. |
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Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysusfraudfish identificationblackfin goosefishRAPD-PCRLophius gastrophysus has important commercial value in Brazil particularly for foreign trade. In this study, we described the optimization of Random Amplified Polymorphic DNA (RAPD) protocol for identification of L. gastrophysus. Different conditions (annealing temperatures, MgCl concentrations, DNA quantity) were tested to find reproducible and adequate profiles. Amplifications performed with primers A01, ² A02 and A03 generate the best RAPD profiles when the conditions were annealing temperature of 36ºC, 25 ng of DNA quantity and 2.5 mM MgCl2. Exact identification of the species and origin of marine products is necessary and RAPD could be used as an accurate, rapid tool to expose commercial fraud.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2005-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612005000400017Food Science and Technology v.25 n.4 2005reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/S0101-20612005000400017info:eu-repo/semantics/openAccessRamella,Micheline S.Kroth,Mariela A.Tagliari,CarolineArisi,Ana Carolina M.eng2006-02-14T00:00:00Zoai:scielo:S0101-20612005000400017Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2006-02-14T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus |
title |
Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus |
spellingShingle |
Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus Ramella,Micheline S. fraud fish identification blackfin goosefish RAPD-PCR |
title_short |
Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus |
title_full |
Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus |
title_fullStr |
Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus |
title_full_unstemmed |
Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus |
title_sort |
Optimization of random amplified polymorphic DNA protocol for molecular identification of Lophius gastrophysus |
author |
Ramella,Micheline S. |
author_facet |
Ramella,Micheline S. Kroth,Mariela A. Tagliari,Caroline Arisi,Ana Carolina M. |
author_role |
author |
author2 |
Kroth,Mariela A. Tagliari,Caroline Arisi,Ana Carolina M. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Ramella,Micheline S. Kroth,Mariela A. Tagliari,Caroline Arisi,Ana Carolina M. |
dc.subject.por.fl_str_mv |
fraud fish identification blackfin goosefish RAPD-PCR |
topic |
fraud fish identification blackfin goosefish RAPD-PCR |
description |
Lophius gastrophysus has important commercial value in Brazil particularly for foreign trade. In this study, we described the optimization of Random Amplified Polymorphic DNA (RAPD) protocol for identification of L. gastrophysus. Different conditions (annealing temperatures, MgCl concentrations, DNA quantity) were tested to find reproducible and adequate profiles. Amplifications performed with primers A01, ² A02 and A03 generate the best RAPD profiles when the conditions were annealing temperature of 36ºC, 25 ng of DNA quantity and 2.5 mM MgCl2. Exact identification of the species and origin of marine products is necessary and RAPD could be used as an accurate, rapid tool to expose commercial fraud. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612005000400017 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612005000400017 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0101-20612005000400017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.25 n.4 2005 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126312353366016 |