Antioxidant activity of black bean (Phaseolus vulgaris L.) protein hydrolysates
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000500023 |
Resumo: | Abstract The objective of this work was to study the effect of enzymatic hydrolysis of black bean protein concentrate using different enzymes. Bean proteins were extracted and hydrolyzed over a period of 120 min using the enzymes pepsin or alcalase. The protein hydrolysates’ molecular weight was assayed by electrophoresis and the antioxidant activity was evaluated by the capturing methods of free radicals ABTS●+ and DPPH. Electrophoretic results showed that the bands above 50 kDa disappeared, when the beans protein was subjected to hydrolysis with pepsin. The bean protein hydrolysate obtained by hydrolysis with alcalase enzyme, showed higher antioxidant activity for inhibition of the radical ABTS●+. However, the hydrolysates obtained by hydrolysis with pepsin had higher antioxidant activity for inhibition of the radical DPPH. The use of pepsin and alcalase enzymes, under the same reaction time, produced black bean protein hydrolysates with different molecular weight profiles and superior antioxidant activity than the native bean protein. |
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Food Science and Technology (Campinas) |
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Antioxidant activity of black bean (Phaseolus vulgaris L.) protein hydrolysatesalcalaseantioxidantmolecular weightpepsinproteinAbstract The objective of this work was to study the effect of enzymatic hydrolysis of black bean protein concentrate using different enzymes. Bean proteins were extracted and hydrolyzed over a period of 120 min using the enzymes pepsin or alcalase. The protein hydrolysates’ molecular weight was assayed by electrophoresis and the antioxidant activity was evaluated by the capturing methods of free radicals ABTS●+ and DPPH. Electrophoretic results showed that the bands above 50 kDa disappeared, when the beans protein was subjected to hydrolysis with pepsin. The bean protein hydrolysate obtained by hydrolysis with alcalase enzyme, showed higher antioxidant activity for inhibition of the radical ABTS●+. However, the hydrolysates obtained by hydrolysis with pepsin had higher antioxidant activity for inhibition of the radical DPPH. The use of pepsin and alcalase enzymes, under the same reaction time, produced black bean protein hydrolysates with different molecular weight profiles and superior antioxidant activity than the native bean protein.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2016-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000500023Food Science and Technology v.36 suppl.1 2016reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/1678-457x.0047info:eu-repo/semantics/openAccessEVANGELHO,Jarine Amaral doBERRIOS,Jose de J.PINTO,Vânia ZanellaANTUNES,Mariana DiasVANIER,Nathan LevienZAVAREZE,Elessandra da Rosaeng2018-10-03T00:00:00Zoai:scielo:S0101-20612016000500023Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2018-10-03T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Antioxidant activity of black bean (Phaseolus vulgaris L.) protein hydrolysates |
title |
Antioxidant activity of black bean (Phaseolus vulgaris L.) protein hydrolysates |
spellingShingle |
Antioxidant activity of black bean (Phaseolus vulgaris L.) protein hydrolysates EVANGELHO,Jarine Amaral do alcalase antioxidant molecular weight pepsin protein |
title_short |
Antioxidant activity of black bean (Phaseolus vulgaris L.) protein hydrolysates |
title_full |
Antioxidant activity of black bean (Phaseolus vulgaris L.) protein hydrolysates |
title_fullStr |
Antioxidant activity of black bean (Phaseolus vulgaris L.) protein hydrolysates |
title_full_unstemmed |
Antioxidant activity of black bean (Phaseolus vulgaris L.) protein hydrolysates |
title_sort |
Antioxidant activity of black bean (Phaseolus vulgaris L.) protein hydrolysates |
author |
EVANGELHO,Jarine Amaral do |
author_facet |
EVANGELHO,Jarine Amaral do BERRIOS,Jose de J. PINTO,Vânia Zanella ANTUNES,Mariana Dias VANIER,Nathan Levien ZAVAREZE,Elessandra da Rosa |
author_role |
author |
author2 |
BERRIOS,Jose de J. PINTO,Vânia Zanella ANTUNES,Mariana Dias VANIER,Nathan Levien ZAVAREZE,Elessandra da Rosa |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
EVANGELHO,Jarine Amaral do BERRIOS,Jose de J. PINTO,Vânia Zanella ANTUNES,Mariana Dias VANIER,Nathan Levien ZAVAREZE,Elessandra da Rosa |
dc.subject.por.fl_str_mv |
alcalase antioxidant molecular weight pepsin protein |
topic |
alcalase antioxidant molecular weight pepsin protein |
description |
Abstract The objective of this work was to study the effect of enzymatic hydrolysis of black bean protein concentrate using different enzymes. Bean proteins were extracted and hydrolyzed over a period of 120 min using the enzymes pepsin or alcalase. The protein hydrolysates’ molecular weight was assayed by electrophoresis and the antioxidant activity was evaluated by the capturing methods of free radicals ABTS●+ and DPPH. Electrophoretic results showed that the bands above 50 kDa disappeared, when the beans protein was subjected to hydrolysis with pepsin. The bean protein hydrolysate obtained by hydrolysis with alcalase enzyme, showed higher antioxidant activity for inhibition of the radical ABTS●+. However, the hydrolysates obtained by hydrolysis with pepsin had higher antioxidant activity for inhibition of the radical DPPH. The use of pepsin and alcalase enzymes, under the same reaction time, produced black bean protein hydrolysates with different molecular weight profiles and superior antioxidant activity than the native bean protein. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000500023 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000500023 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-457x.0047 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.36 suppl.1 2016 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126320709468160 |