Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion

Detalhes bibliográficos
Autor(a) principal: ZOU,Tiantian
Data de Publicação: 2022
Outros Autores: WU,Jianjiang, YANG,Long, TAIWANGU,Tailaiti, CHEN,Siyu, WANG,Jiang
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Food Science and Technology (Campinas)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100770
Resumo: Abstract To evaluate the effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion (I/R). Mice were divided into 1) Blank (Sham) group; 2) Ischemia-reperfusion (I/R) group; 3) I/R+2ME2 group (I/R+HIF-1α blocker 2ME2. Compared with Sham group, in I/R group and I/R+2ME2 group, HR slowed down, LVDP and +dp/dtmax decreased, LVEDP, myocardial infarction area, and ROS generation rate was significantly increased; whereas the mitochondrial respiratory function (State3, RCR) and mitochondrial respiratory enzyme activity (NADH-OX, Cytc-OX and SUC-OX) were significantly reduced. Compared with the I/R group, the respiratory function of myocardial mtochondria (State3, RCR) and respiratory enzyme activity of myocardial (NADH-OX, Cytc-OX and SUC-OX) were significantly reduced in the I/R+2ME2 group; and the levels of p-HIF-1α and p-VEGF in the I/R+2ME2 group were significantly decreased. HIF-1α pathway upregulate the respiratory enzyme activity of myocardial and respiratory function of I/R myocardial mitochondria.
id SBCTA-1_5607df389d6328643b1771878adeef7c
oai_identifier_str oai:scielo:S0101-20612022000100770
network_acronym_str SBCTA-1
network_name_str Food Science and Technology (Campinas)
repository_id_str
spelling Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusionhypoxia inducible factor-1αmyocardial ischemia-reperfusion injuryaged myocardiummitochondrial respiratory functionAbstract To evaluate the effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion (I/R). Mice were divided into 1) Blank (Sham) group; 2) Ischemia-reperfusion (I/R) group; 3) I/R+2ME2 group (I/R+HIF-1α blocker 2ME2. Compared with Sham group, in I/R group and I/R+2ME2 group, HR slowed down, LVDP and +dp/dtmax decreased, LVEDP, myocardial infarction area, and ROS generation rate was significantly increased; whereas the mitochondrial respiratory function (State3, RCR) and mitochondrial respiratory enzyme activity (NADH-OX, Cytc-OX and SUC-OX) were significantly reduced. Compared with the I/R group, the respiratory function of myocardial mtochondria (State3, RCR) and respiratory enzyme activity of myocardial (NADH-OX, Cytc-OX and SUC-OX) were significantly reduced in the I/R+2ME2 group; and the levels of p-HIF-1α and p-VEGF in the I/R+2ME2 group were significantly decreased. HIF-1α pathway upregulate the respiratory enzyme activity of myocardial and respiratory function of I/R myocardial mitochondria.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100770Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.59121info:eu-repo/semantics/openAccessZOU,TiantianWU,JianjiangYANG,LongTAIWANGU,TailaitiCHEN,SiyuWANG,Jiangeng2022-02-22T00:00:00Zoai:scielo:S0101-20612022000100770Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-02-22T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false
dc.title.none.fl_str_mv Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion
title Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion
spellingShingle Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion
ZOU,Tiantian
hypoxia inducible factor-1α
myocardial ischemia-reperfusion injury
aged myocardium
mitochondrial respiratory function
title_short Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion
title_full Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion
title_fullStr Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion
title_full_unstemmed Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion
title_sort Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion
author ZOU,Tiantian
author_facet ZOU,Tiantian
WU,Jianjiang
YANG,Long
TAIWANGU,Tailaiti
CHEN,Siyu
WANG,Jiang
author_role author
author2 WU,Jianjiang
YANG,Long
TAIWANGU,Tailaiti
CHEN,Siyu
WANG,Jiang
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv ZOU,Tiantian
WU,Jianjiang
YANG,Long
TAIWANGU,Tailaiti
CHEN,Siyu
WANG,Jiang
dc.subject.por.fl_str_mv hypoxia inducible factor-1α
myocardial ischemia-reperfusion injury
aged myocardium
mitochondrial respiratory function
topic hypoxia inducible factor-1α
myocardial ischemia-reperfusion injury
aged myocardium
mitochondrial respiratory function
description Abstract To evaluate the effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion (I/R). Mice were divided into 1) Blank (Sham) group; 2) Ischemia-reperfusion (I/R) group; 3) I/R+2ME2 group (I/R+HIF-1α blocker 2ME2. Compared with Sham group, in I/R group and I/R+2ME2 group, HR slowed down, LVDP and +dp/dtmax decreased, LVEDP, myocardial infarction area, and ROS generation rate was significantly increased; whereas the mitochondrial respiratory function (State3, RCR) and mitochondrial respiratory enzyme activity (NADH-OX, Cytc-OX and SUC-OX) were significantly reduced. Compared with the I/R group, the respiratory function of myocardial mtochondria (State3, RCR) and respiratory enzyme activity of myocardial (NADH-OX, Cytc-OX and SUC-OX) were significantly reduced in the I/R+2ME2 group; and the levels of p-HIF-1α and p-VEGF in the I/R+2ME2 group were significantly decreased. HIF-1α pathway upregulate the respiratory enzyme activity of myocardial and respiratory function of I/R myocardial mitochondria.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100770
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100770
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/fst.59121
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
publisher.none.fl_str_mv Sociedade Brasileira de Ciência e Tecnologia de Alimentos
dc.source.none.fl_str_mv Food Science and Technology v.42 2022
reponame:Food Science and Technology (Campinas)
instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron:SBCTA
instname_str Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
instacron_str SBCTA
institution SBCTA
reponame_str Food Science and Technology (Campinas)
collection Food Science and Technology (Campinas)
repository.name.fl_str_mv Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)
repository.mail.fl_str_mv ||revista@sbcta.org.br
_version_ 1752126332866658304

Registros relacionados