Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100770 |
Resumo: | Abstract To evaluate the effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion (I/R). Mice were divided into 1) Blank (Sham) group; 2) Ischemia-reperfusion (I/R) group; 3) I/R+2ME2 group (I/R+HIF-1α blocker 2ME2. Compared with Sham group, in I/R group and I/R+2ME2 group, HR slowed down, LVDP and +dp/dtmax decreased, LVEDP, myocardial infarction area, and ROS generation rate was significantly increased; whereas the mitochondrial respiratory function (State3, RCR) and mitochondrial respiratory enzyme activity (NADH-OX, Cytc-OX and SUC-OX) were significantly reduced. Compared with the I/R group, the respiratory function of myocardial mtochondria (State3, RCR) and respiratory enzyme activity of myocardial (NADH-OX, Cytc-OX and SUC-OX) were significantly reduced in the I/R+2ME2 group; and the levels of p-HIF-1α and p-VEGF in the I/R+2ME2 group were significantly decreased. HIF-1α pathway upregulate the respiratory enzyme activity of myocardial and respiratory function of I/R myocardial mitochondria. |
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Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusionhypoxia inducible factor-1αmyocardial ischemia-reperfusion injuryaged myocardiummitochondrial respiratory functionAbstract To evaluate the effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion (I/R). Mice were divided into 1) Blank (Sham) group; 2) Ischemia-reperfusion (I/R) group; 3) I/R+2ME2 group (I/R+HIF-1α blocker 2ME2. Compared with Sham group, in I/R group and I/R+2ME2 group, HR slowed down, LVDP and +dp/dtmax decreased, LVEDP, myocardial infarction area, and ROS generation rate was significantly increased; whereas the mitochondrial respiratory function (State3, RCR) and mitochondrial respiratory enzyme activity (NADH-OX, Cytc-OX and SUC-OX) were significantly reduced. Compared with the I/R group, the respiratory function of myocardial mtochondria (State3, RCR) and respiratory enzyme activity of myocardial (NADH-OX, Cytc-OX and SUC-OX) were significantly reduced in the I/R+2ME2 group; and the levels of p-HIF-1α and p-VEGF in the I/R+2ME2 group were significantly decreased. HIF-1α pathway upregulate the respiratory enzyme activity of myocardial and respiratory function of I/R myocardial mitochondria.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100770Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.59121info:eu-repo/semantics/openAccessZOU,TiantianWU,JianjiangYANG,LongTAIWANGU,TailaitiCHEN,SiyuWANG,Jiangeng2022-02-22T00:00:00Zoai:scielo:S0101-20612022000100770Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-02-22T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion |
title |
Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion |
spellingShingle |
Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion ZOU,Tiantian hypoxia inducible factor-1α myocardial ischemia-reperfusion injury aged myocardium mitochondrial respiratory function |
title_short |
Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion |
title_full |
Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion |
title_fullStr |
Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion |
title_full_unstemmed |
Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion |
title_sort |
Effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion |
author |
ZOU,Tiantian |
author_facet |
ZOU,Tiantian WU,Jianjiang YANG,Long TAIWANGU,Tailaiti CHEN,Siyu WANG,Jiang |
author_role |
author |
author2 |
WU,Jianjiang YANG,Long TAIWANGU,Tailaiti CHEN,Siyu WANG,Jiang |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
ZOU,Tiantian WU,Jianjiang YANG,Long TAIWANGU,Tailaiti CHEN,Siyu WANG,Jiang |
dc.subject.por.fl_str_mv |
hypoxia inducible factor-1α myocardial ischemia-reperfusion injury aged myocardium mitochondrial respiratory function |
topic |
hypoxia inducible factor-1α myocardial ischemia-reperfusion injury aged myocardium mitochondrial respiratory function |
description |
Abstract To evaluate the effects of HIF-1α overexpression on mitochondrial function in aged mice with myocardial ischemia-reperfusion (I/R). Mice were divided into 1) Blank (Sham) group; 2) Ischemia-reperfusion (I/R) group; 3) I/R+2ME2 group (I/R+HIF-1α blocker 2ME2. Compared with Sham group, in I/R group and I/R+2ME2 group, HR slowed down, LVDP and +dp/dtmax decreased, LVEDP, myocardial infarction area, and ROS generation rate was significantly increased; whereas the mitochondrial respiratory function (State3, RCR) and mitochondrial respiratory enzyme activity (NADH-OX, Cytc-OX and SUC-OX) were significantly reduced. Compared with the I/R group, the respiratory function of myocardial mtochondria (State3, RCR) and respiratory enzyme activity of myocardial (NADH-OX, Cytc-OX and SUC-OX) were significantly reduced in the I/R+2ME2 group; and the levels of p-HIF-1α and p-VEGF in the I/R+2ME2 group were significantly decreased. HIF-1α pathway upregulate the respiratory enzyme activity of myocardial and respiratory function of I/R myocardial mitochondria. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100770 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100770 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.59121 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.42 2022 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
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1752126332866658304 |