Survival of Shiga toxin-producing Escherichia coli O157:H7 in Minas frescal cheese
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000100108 |
Resumo: | Shiga toxin-producing Escherichia coli (STEC) O157:H7 strains (isolated by cattle’s faeces and a reference strain, EDL933), were inoculated into pasteurized milk (102 and 103 cells.mL–1) to prepare the Minas frescal cheese. As control was used uninfected milk. Physicochemical and microbiological analyses were performed to milk and elaborated cheese. The O157:H7 strains were quantified in the stages of cheese processing and during 0, 2, 4, 5, 7, 10 and 15 storage days at 8 °C onto Sorbitol MacConkey Agar supplemented with potassium tellurite and cefixime (CT-SMAC). O157:H7 was not present in the pasteurised milk prior to the artificial inoculation. At the end of the processing the cheese had 10 to 100 times more STEC O157:H7 than the initial inoculum. During the storage, the Minas frescal cheese exhibited the largest population increase on the 4th and 5th day when inoculated with 102 and 103 cells.mL–1, respectively. Additionally, viable cells were found up to the 10th and 15th day, according to the amount of initial inoculum. This number of cells is able to cause infection in humans, and therefore, Minas frescal cheese, even when stored under refrigeration, is a potential vehicle of disease caused by STEC O157:H7. |
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Survival of Shiga toxin-producing Escherichia coli O157:H7 in Minas frescal cheesecheesepsychrotrophic bacteriafoodborne diseaseShiga toxin-producing Escherichia coli (STEC) O157:H7 strains (isolated by cattle’s faeces and a reference strain, EDL933), were inoculated into pasteurized milk (102 and 103 cells.mL–1) to prepare the Minas frescal cheese. As control was used uninfected milk. Physicochemical and microbiological analyses were performed to milk and elaborated cheese. The O157:H7 strains were quantified in the stages of cheese processing and during 0, 2, 4, 5, 7, 10 and 15 storage days at 8 °C onto Sorbitol MacConkey Agar supplemented with potassium tellurite and cefixime (CT-SMAC). O157:H7 was not present in the pasteurised milk prior to the artificial inoculation. At the end of the processing the cheese had 10 to 100 times more STEC O157:H7 than the initial inoculum. During the storage, the Minas frescal cheese exhibited the largest population increase on the 4th and 5th day when inoculated with 102 and 103 cells.mL–1, respectively. Additionally, viable cells were found up to the 10th and 15th day, according to the amount of initial inoculum. This number of cells is able to cause infection in humans, and therefore, Minas frescal cheese, even when stored under refrigeration, is a potential vehicle of disease caused by STEC O157:H7.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2015-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000100108Food Science and Technology v.35 n.1 2015reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/1678-457X.6528info:eu-repo/semantics/openAccessFrozi,Jesieli BrazDomingues,Josiane RobertoEsper,Luciana Maria RamiresCorrêa da Rosa,Joel MaurícioSilva,Ana Luiza Sant’Anna da CostaGonzalez,Alice Gonçalves Martinseng2015-05-06T00:00:00Zoai:scielo:S0101-20612015000100108Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2015-05-06T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Survival of Shiga toxin-producing Escherichia coli O157:H7 in Minas frescal cheese |
title |
Survival of Shiga toxin-producing Escherichia coli O157:H7 in Minas frescal cheese |
spellingShingle |
Survival of Shiga toxin-producing Escherichia coli O157:H7 in Minas frescal cheese Frozi,Jesieli Braz cheese psychrotrophic bacteria foodborne disease |
title_short |
Survival of Shiga toxin-producing Escherichia coli O157:H7 in Minas frescal cheese |
title_full |
Survival of Shiga toxin-producing Escherichia coli O157:H7 in Minas frescal cheese |
title_fullStr |
Survival of Shiga toxin-producing Escherichia coli O157:H7 in Minas frescal cheese |
title_full_unstemmed |
Survival of Shiga toxin-producing Escherichia coli O157:H7 in Minas frescal cheese |
title_sort |
Survival of Shiga toxin-producing Escherichia coli O157:H7 in Minas frescal cheese |
author |
Frozi,Jesieli Braz |
author_facet |
Frozi,Jesieli Braz Domingues,Josiane Roberto Esper,Luciana Maria Ramires Corrêa da Rosa,Joel Maurício Silva,Ana Luiza Sant’Anna da Costa Gonzalez,Alice Gonçalves Martins |
author_role |
author |
author2 |
Domingues,Josiane Roberto Esper,Luciana Maria Ramires Corrêa da Rosa,Joel Maurício Silva,Ana Luiza Sant’Anna da Costa Gonzalez,Alice Gonçalves Martins |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Frozi,Jesieli Braz Domingues,Josiane Roberto Esper,Luciana Maria Ramires Corrêa da Rosa,Joel Maurício Silva,Ana Luiza Sant’Anna da Costa Gonzalez,Alice Gonçalves Martins |
dc.subject.por.fl_str_mv |
cheese psychrotrophic bacteria foodborne disease |
topic |
cheese psychrotrophic bacteria foodborne disease |
description |
Shiga toxin-producing Escherichia coli (STEC) O157:H7 strains (isolated by cattle’s faeces and a reference strain, EDL933), were inoculated into pasteurized milk (102 and 103 cells.mL–1) to prepare the Minas frescal cheese. As control was used uninfected milk. Physicochemical and microbiological analyses were performed to milk and elaborated cheese. The O157:H7 strains were quantified in the stages of cheese processing and during 0, 2, 4, 5, 7, 10 and 15 storage days at 8 °C onto Sorbitol MacConkey Agar supplemented with potassium tellurite and cefixime (CT-SMAC). O157:H7 was not present in the pasteurised milk prior to the artificial inoculation. At the end of the processing the cheese had 10 to 100 times more STEC O157:H7 than the initial inoculum. During the storage, the Minas frescal cheese exhibited the largest population increase on the 4th and 5th day when inoculated with 102 and 103 cells.mL–1, respectively. Additionally, viable cells were found up to the 10th and 15th day, according to the amount of initial inoculum. This number of cells is able to cause infection in humans, and therefore, Minas frescal cheese, even when stored under refrigeration, is a potential vehicle of disease caused by STEC O157:H7. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000100108 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612015000100108 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1678-457X.6528 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.35 n.1 2015 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
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1752126319446982656 |