Metabolome and transcriptome sequencing analysis reveals anthocyanins in the red flowers of black locust (Robinia pseudoacacia L.)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101066 |
Resumo: | Abstract Black locust (Robinia pseudoacacia L.) flowers display a white or red color. The white flowers are a popular food in Chinese markets, while the red flowers have high ornamental value. In this study, combined analysis of the transcriptome and flavonoid metabolome was performed to investigate the key genes and metabolites involved in flower pigmentation in the red and white flowers of black locust. A total of 308 flavonoid metabolites were identified, and five anthocyanins were significantly higher in the red flowers compared to the white flowers. Transcriptome sequencing yielded about 66 Gb data, from which 53,992 unigenes were assembled. Compared with the white flowers, 1,394 unigenes were up-regulated and 1,201 unigenes were down-regulated in the red flowers. Three anthocyanin structural genes, F3'H, ANS, and 3GT, were significantly up-regulated in the red flowers, representing the key genes for anthocyanin accumulation in the red flowers. Nine MYB and four bHLH genes were up-regulated in the red flowers, representing the candidate genes regulating the anthocyanin pathway. These results provide a theoretical basis for the development of black locust flowers as a food and also provide a foundation for the study of anthocyanin regulation in black locust. |
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Metabolome and transcriptome sequencing analysis reveals anthocyanins in the red flowers of black locust (Robinia pseudoacacia L.)black locustRobinia pseudoacacia L.red flowerflavonoidsanthocyaninsmetabolometranscriptomeAbstract Black locust (Robinia pseudoacacia L.) flowers display a white or red color. The white flowers are a popular food in Chinese markets, while the red flowers have high ornamental value. In this study, combined analysis of the transcriptome and flavonoid metabolome was performed to investigate the key genes and metabolites involved in flower pigmentation in the red and white flowers of black locust. A total of 308 flavonoid metabolites were identified, and five anthocyanins were significantly higher in the red flowers compared to the white flowers. Transcriptome sequencing yielded about 66 Gb data, from which 53,992 unigenes were assembled. Compared with the white flowers, 1,394 unigenes were up-regulated and 1,201 unigenes were down-regulated in the red flowers. Three anthocyanin structural genes, F3'H, ANS, and 3GT, were significantly up-regulated in the red flowers, representing the key genes for anthocyanin accumulation in the red flowers. Nine MYB and four bHLH genes were up-regulated in the red flowers, representing the candidate genes regulating the anthocyanin pathway. These results provide a theoretical basis for the development of black locust flowers as a food and also provide a foundation for the study of anthocyanin regulation in black locust.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101066Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.08922info:eu-repo/semantics/openAccessZHANG,YanzhaoLU,XiJIA,LinglanJIN,HuanhuanCHENG,Yanweieng2022-04-12T00:00:00Zoai:scielo:S0101-20612022000101066Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-04-12T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Metabolome and transcriptome sequencing analysis reveals anthocyanins in the red flowers of black locust (Robinia pseudoacacia L.) |
title |
Metabolome and transcriptome sequencing analysis reveals anthocyanins in the red flowers of black locust (Robinia pseudoacacia L.) |
spellingShingle |
Metabolome and transcriptome sequencing analysis reveals anthocyanins in the red flowers of black locust (Robinia pseudoacacia L.) ZHANG,Yanzhao black locust Robinia pseudoacacia L. red flower flavonoids anthocyanins metabolome transcriptome |
title_short |
Metabolome and transcriptome sequencing analysis reveals anthocyanins in the red flowers of black locust (Robinia pseudoacacia L.) |
title_full |
Metabolome and transcriptome sequencing analysis reveals anthocyanins in the red flowers of black locust (Robinia pseudoacacia L.) |
title_fullStr |
Metabolome and transcriptome sequencing analysis reveals anthocyanins in the red flowers of black locust (Robinia pseudoacacia L.) |
title_full_unstemmed |
Metabolome and transcriptome sequencing analysis reveals anthocyanins in the red flowers of black locust (Robinia pseudoacacia L.) |
title_sort |
Metabolome and transcriptome sequencing analysis reveals anthocyanins in the red flowers of black locust (Robinia pseudoacacia L.) |
author |
ZHANG,Yanzhao |
author_facet |
ZHANG,Yanzhao LU,Xi JIA,Linglan JIN,Huanhuan CHENG,Yanwei |
author_role |
author |
author2 |
LU,Xi JIA,Linglan JIN,Huanhuan CHENG,Yanwei |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
ZHANG,Yanzhao LU,Xi JIA,Linglan JIN,Huanhuan CHENG,Yanwei |
dc.subject.por.fl_str_mv |
black locust Robinia pseudoacacia L. red flower flavonoids anthocyanins metabolome transcriptome |
topic |
black locust Robinia pseudoacacia L. red flower flavonoids anthocyanins metabolome transcriptome |
description |
Abstract Black locust (Robinia pseudoacacia L.) flowers display a white or red color. The white flowers are a popular food in Chinese markets, while the red flowers have high ornamental value. In this study, combined analysis of the transcriptome and flavonoid metabolome was performed to investigate the key genes and metabolites involved in flower pigmentation in the red and white flowers of black locust. A total of 308 flavonoid metabolites were identified, and five anthocyanins were significantly higher in the red flowers compared to the white flowers. Transcriptome sequencing yielded about 66 Gb data, from which 53,992 unigenes were assembled. Compared with the white flowers, 1,394 unigenes were up-regulated and 1,201 unigenes were down-regulated in the red flowers. Three anthocyanin structural genes, F3'H, ANS, and 3GT, were significantly up-regulated in the red flowers, representing the key genes for anthocyanin accumulation in the red flowers. Nine MYB and four bHLH genes were up-regulated in the red flowers, representing the candidate genes regulating the anthocyanin pathway. These results provide a theoretical basis for the development of black locust flowers as a food and also provide a foundation for the study of anthocyanin regulation in black locust. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101066 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101066 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.08922 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.42 2022 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126334122852352 |