UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000100225 |
Resumo: | ABSTRACT: Two analytical methods were developed, one utilizing sensitive UPLC-MS/MS method while other being the spectrophotometric method exploiting the Dushman reaction. Both methods were validated based on ICH guidelines and were successfully applied to quantitate the drug in marketed formulation. UPLC-MS/MS method responded linearly in the range of 1.6 µgmL-1-6.4 µgmL-1 while spectrophotometric procedure followed the Beer’s law in the concentration range of 30-100 µgmL-1. UPLC-MS/MS has been found to have a limit of quantitation of 0.97 µgmL-1 while spectrophotometric method was found to quantitate 14.00 µgmL-1 correctly with high precision. Both the methods showed an excellent recovery of 99.36%-100.91% (% RSD 1.05-1.73) and 98.64%-99.73% (%RSD 0.67-1.68) for UPLC-MS/MS and spectrophotometric, respectively. |
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UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulationCeftazidimeUPLC-MS/MSmethod developmentspectrophotometryABSTRACT: Two analytical methods were developed, one utilizing sensitive UPLC-MS/MS method while other being the spectrophotometric method exploiting the Dushman reaction. Both methods were validated based on ICH guidelines and were successfully applied to quantitate the drug in marketed formulation. UPLC-MS/MS method responded linearly in the range of 1.6 µgmL-1-6.4 µgmL-1 while spectrophotometric procedure followed the Beer’s law in the concentration range of 30-100 µgmL-1. UPLC-MS/MS has been found to have a limit of quantitation of 0.97 µgmL-1 while spectrophotometric method was found to quantitate 14.00 µgmL-1 correctly with high precision. Both the methods showed an excellent recovery of 99.36%-100.91% (% RSD 1.05-1.73) and 98.64%-99.73% (%RSD 0.67-1.68) for UPLC-MS/MS and spectrophotometric, respectively.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2021-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000100225Food Science and Technology v.41 n.1 2021reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.07020info:eu-repo/semantics/openAccessSIDDIQUI,Masoom RazaHAKAMI,Afnan Ali HussainWABAIDUR,Saikh MohammadALOTHMAN,Zeid AbdullahKHAN,Moonis AliHUSAIN,Fohad Maboodeng2021-03-18T00:00:00Zoai:scielo:S0101-20612021000100225Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2021-03-18T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation |
title |
UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation |
spellingShingle |
UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation SIDDIQUI,Masoom Raza Ceftazidime UPLC-MS/MS method development spectrophotometry |
title_short |
UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation |
title_full |
UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation |
title_fullStr |
UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation |
title_full_unstemmed |
UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation |
title_sort |
UPLC-MS/MS and Dushman reaction based spectrophotometric method for determination of Ceftazidime, an antibiotic, in medicinal formulation |
author |
SIDDIQUI,Masoom Raza |
author_facet |
SIDDIQUI,Masoom Raza HAKAMI,Afnan Ali Hussain WABAIDUR,Saikh Mohammad ALOTHMAN,Zeid Abdullah KHAN,Moonis Ali HUSAIN,Fohad Mabood |
author_role |
author |
author2 |
HAKAMI,Afnan Ali Hussain WABAIDUR,Saikh Mohammad ALOTHMAN,Zeid Abdullah KHAN,Moonis Ali HUSAIN,Fohad Mabood |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
SIDDIQUI,Masoom Raza HAKAMI,Afnan Ali Hussain WABAIDUR,Saikh Mohammad ALOTHMAN,Zeid Abdullah KHAN,Moonis Ali HUSAIN,Fohad Mabood |
dc.subject.por.fl_str_mv |
Ceftazidime UPLC-MS/MS method development spectrophotometry |
topic |
Ceftazidime UPLC-MS/MS method development spectrophotometry |
description |
ABSTRACT: Two analytical methods were developed, one utilizing sensitive UPLC-MS/MS method while other being the spectrophotometric method exploiting the Dushman reaction. Both methods were validated based on ICH guidelines and were successfully applied to quantitate the drug in marketed formulation. UPLC-MS/MS method responded linearly in the range of 1.6 µgmL-1-6.4 µgmL-1 while spectrophotometric procedure followed the Beer’s law in the concentration range of 30-100 µgmL-1. UPLC-MS/MS has been found to have a limit of quantitation of 0.97 µgmL-1 while spectrophotometric method was found to quantitate 14.00 µgmL-1 correctly with high precision. Both the methods showed an excellent recovery of 99.36%-100.91% (% RSD 1.05-1.73) and 98.64%-99.73% (%RSD 0.67-1.68) for UPLC-MS/MS and spectrophotometric, respectively. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000100225 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000100225 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.07020 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.41 n.1 2021 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126328120803328 |