Hydroxysafflor yellow A from safflower (Carthamus tinctorius L.) prevents cerebral ischemia-reperfusion injury in rats

• Autor(a) principal: WANG,Yunfeng
• Data de Publicação: 2022
• Outros Autores: DU,Yuanmin, LI,Jin, XU,Kang
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Food Science and Technology (Campinas)
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000100637
• topic: hydroxysafflor yellow A; cerebral ischemia-reperfusion injury; rats; p38 MAPK; NF-κB

Abstract In this study we extracted hydroxysafflor yellow A (HSYA) from safflower and investigate its preventive effect on cerebral ischemia-reperfusion injury (CIRI) in rats. HSYA with 89.78% purity was extracted from safflower. Ninety rats were divided into sham-operated, model, low-dose HSYA, high-dose HSYA and nimodipine groups. The CIRI model was established in latter three groups. After 30 min from ischemia beginning, the low-dose and high-dose HSYA groups were treated with 2 and 4 mg/kg HSYA via tail-vein injection, respectively. The nimodipine group was treated with 2 mg/kg nimodipine. Results showed that, at 24 h after ischemia, compared with model group, in high-dose HSYA and nimodipine groups the neurological symptom score was significantly decreased (P < 0.05), the brain water content was significantly decreased (P < 0.05), the brain infarction area percentage was significantly decreased (P < 0.05), the serum IL-6 and IL-1β levels were significantly decreased (P < 0.05), and the brain tissue phosphorylated p38 mitogen-activated protein kinase (p-p38 MAPK) and phosphorylated nuclear factor kappa B (p-NF-κB) p65 protein expression levels were significantly decreased (P < 0.05). In conclusion, HSYA can inhibit the p38 MAPK/NF-κB pathway and reduce the inflammatory response, thus exerting the preventive effect on CIRI in rats.