Rivastigmine prevents injury induced by ischemia and reperfusion in rat liver

Detalhes bibliográficos
Autor(a) principal: Chies,Agnaldo Bruno
Data de Publicação: 2018
Outros Autores: Nakazato,Paula Carolina Grande, Spadella,Maria Angélica, Zorzi,Patrícia, Gomes,Maria Cecília Jordani, D’Albuquerque,Luiz Augusto Carneiro, Castro-e-Silva,Orlando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000900775
Resumo: Abstract Purpose: To evaluate whether pre-treatment with rivastigmine is able to attenuate the I/R induced lesions in rat liver. Methods: SHAM animals or those submitted to I/R, non-treated or pre-treated with rivastigminine (2mg/kg) either 50 or 15 minutes before ischemia, were used. After I/R protocol, these animals were killed and their livers were harvested to measurement of the mitochondrial swelling as well as the malondialdehyde (MDA), nitrite and nitrate tissue concentration. Blood was also harvested for serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) determinations. Results: I/R promoted a significant increase of mitochondrial swelling in the studied animals. This increase of mitochondrial swelling was partially prevented by rivastigmine, but only if administered 50 minutes before ischemia. No significant modification of MDA, nitrite or nitrate tissue concentrations was observed in consequence of I/R, followed or not by rivastigmine treatments. In addition, I/R elevated both AST and ALT. These elevations of serum enzymes were not reversed by the different rivastigmine treatments. Conclusions: Rivastigmine administered 50 minutes before ischemia attenuates I/R-induced mitochondrial swelling, that indicates liver injury. This protective effect may be related to a greater stimulation of α7nAChR present in the Kupffer cells by the non-methabolized ACh, leading to an attenuation of I/R-induced inflammation.
id SBDPC-1_630ee13ba1f7026677cb65720d3b46f8
oai_identifier_str oai:scielo:S0102-86502018000900775
network_acronym_str SBDPC-1
network_name_str Acta Cirúrgica Brasileira (Online)
repository_id_str
spelling Rivastigmine prevents injury induced by ischemia and reperfusion in rat liverRivastigmineMitochondriaLiverIschemiaReperfusionRatsAbstract Purpose: To evaluate whether pre-treatment with rivastigmine is able to attenuate the I/R induced lesions in rat liver. Methods: SHAM animals or those submitted to I/R, non-treated or pre-treated with rivastigminine (2mg/kg) either 50 or 15 minutes before ischemia, were used. After I/R protocol, these animals were killed and their livers were harvested to measurement of the mitochondrial swelling as well as the malondialdehyde (MDA), nitrite and nitrate tissue concentration. Blood was also harvested for serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) determinations. Results: I/R promoted a significant increase of mitochondrial swelling in the studied animals. This increase of mitochondrial swelling was partially prevented by rivastigmine, but only if administered 50 minutes before ischemia. No significant modification of MDA, nitrite or nitrate tissue concentrations was observed in consequence of I/R, followed or not by rivastigmine treatments. In addition, I/R elevated both AST and ALT. These elevations of serum enzymes were not reversed by the different rivastigmine treatments. Conclusions: Rivastigmine administered 50 minutes before ischemia attenuates I/R-induced mitochondrial swelling, that indicates liver injury. This protective effect may be related to a greater stimulation of α7nAChR present in the Kupffer cells by the non-methabolized ACh, leading to an attenuation of I/R-induced inflammation.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2018-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000900775Acta Cirúrgica Brasileira v.33 n.9 2018reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/s0102-865020180090000005info:eu-repo/semantics/openAccessChies,Agnaldo BrunoNakazato,Paula Carolina GrandeSpadella,Maria AngélicaZorzi,PatríciaGomes,Maria Cecília JordaniD’Albuquerque,Luiz Augusto CarneiroCastro-e-Silva,Orlandoeng2018-10-09T00:00:00Zoai:scielo:S0102-86502018000900775Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2018-10-09T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Rivastigmine prevents injury induced by ischemia and reperfusion in rat liver
title Rivastigmine prevents injury induced by ischemia and reperfusion in rat liver
spellingShingle Rivastigmine prevents injury induced by ischemia and reperfusion in rat liver
Chies,Agnaldo Bruno
Rivastigmine
Mitochondria
Liver
Ischemia
Reperfusion
Rats
title_short Rivastigmine prevents injury induced by ischemia and reperfusion in rat liver
title_full Rivastigmine prevents injury induced by ischemia and reperfusion in rat liver
title_fullStr Rivastigmine prevents injury induced by ischemia and reperfusion in rat liver
title_full_unstemmed Rivastigmine prevents injury induced by ischemia and reperfusion in rat liver
title_sort Rivastigmine prevents injury induced by ischemia and reperfusion in rat liver
author Chies,Agnaldo Bruno
author_facet Chies,Agnaldo Bruno
Nakazato,Paula Carolina Grande
Spadella,Maria Angélica
Zorzi,Patrícia
Gomes,Maria Cecília Jordani
D’Albuquerque,Luiz Augusto Carneiro
Castro-e-Silva,Orlando
author_role author
author2 Nakazato,Paula Carolina Grande
Spadella,Maria Angélica
Zorzi,Patrícia
Gomes,Maria Cecília Jordani
D’Albuquerque,Luiz Augusto Carneiro
Castro-e-Silva,Orlando
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Chies,Agnaldo Bruno
Nakazato,Paula Carolina Grande
Spadella,Maria Angélica
Zorzi,Patrícia
Gomes,Maria Cecília Jordani
D’Albuquerque,Luiz Augusto Carneiro
Castro-e-Silva,Orlando
dc.subject.por.fl_str_mv Rivastigmine
Mitochondria
Liver
Ischemia
Reperfusion
Rats
topic Rivastigmine
Mitochondria
Liver
Ischemia
Reperfusion
Rats
description Abstract Purpose: To evaluate whether pre-treatment with rivastigmine is able to attenuate the I/R induced lesions in rat liver. Methods: SHAM animals or those submitted to I/R, non-treated or pre-treated with rivastigminine (2mg/kg) either 50 or 15 minutes before ischemia, were used. After I/R protocol, these animals were killed and their livers were harvested to measurement of the mitochondrial swelling as well as the malondialdehyde (MDA), nitrite and nitrate tissue concentration. Blood was also harvested for serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) determinations. Results: I/R promoted a significant increase of mitochondrial swelling in the studied animals. This increase of mitochondrial swelling was partially prevented by rivastigmine, but only if administered 50 minutes before ischemia. No significant modification of MDA, nitrite or nitrate tissue concentrations was observed in consequence of I/R, followed or not by rivastigmine treatments. In addition, I/R elevated both AST and ALT. These elevations of serum enzymes were not reversed by the different rivastigmine treatments. Conclusions: Rivastigmine administered 50 minutes before ischemia attenuates I/R-induced mitochondrial swelling, that indicates liver injury. This protective effect may be related to a greater stimulation of α7nAChR present in the Kupffer cells by the non-methabolized ACh, leading to an attenuation of I/R-induced inflammation.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000900775
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502018000900775
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s0102-865020180090000005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.33 n.9 2018
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
_version_ 1752126444963627008