Proteomic analysis of the inhibitory effect of chitosan on Penicillium expansum
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100250 |
Resumo: | Abstract The antimicrobial effect of chitosan on Penicillium expansum, a major postharvest pathogen of pome fruit, and the possible mechanisms involved in its effect were examined in this study. Chitosan strongly inhibited spore germination and hyphal growth of P. expansum. Light microscopy and transmission electron microscopy observations revealed that chitosan also caused morphological changes in hyphae and conidia, such as abnormal branching and vacuolation. Proteomic changes in P. expansum after chitosan treatment were analyzed by two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) analysis, and 26 proteins were ambiguously identified and categorized based on their putative biological function. Proteins related to DNA or protein biosynthesis, carbohydrate metabolism and energy production were decreased in relative protein abundance, while proteins involved in antibiotics resistance and defense response increased in relative protein abundance. Changes in abundance of these identified proteins were in accordance to the observed physiological and morphological changes of the fungi cells. Altogether, these experimental results provide a detailed illustration of the responses to chitosan in P. expansum, and widen our knowledge on the potential antifungal mechanisms of chitosan. |
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Proteomic analysis of the inhibitory effect of chitosan on Penicillium expansumchitosanPenicillium expansumantifungal activityproteomicsAbstract The antimicrobial effect of chitosan on Penicillium expansum, a major postharvest pathogen of pome fruit, and the possible mechanisms involved in its effect were examined in this study. Chitosan strongly inhibited spore germination and hyphal growth of P. expansum. Light microscopy and transmission electron microscopy observations revealed that chitosan also caused morphological changes in hyphae and conidia, such as abnormal branching and vacuolation. Proteomic changes in P. expansum after chitosan treatment were analyzed by two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) analysis, and 26 proteins were ambiguously identified and categorized based on their putative biological function. Proteins related to DNA or protein biosynthesis, carbohydrate metabolism and energy production were decreased in relative protein abundance, while proteins involved in antibiotics resistance and defense response increased in relative protein abundance. Changes in abundance of these identified proteins were in accordance to the observed physiological and morphological changes of the fungi cells. Altogether, these experimental results provide a detailed illustration of the responses to chitosan in P. expansum, and widen our knowledge on the potential antifungal mechanisms of chitosan.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2020-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100250Food Science and Technology v.40 n.1 2020reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.40418info:eu-repo/semantics/openAccessLI,MingyanCHEN,ChiXIA,XiaoshuangGARBA,BetchemSHANG,LinlinWANG,Yuneng2020-05-29T00:00:00Zoai:scielo:S0101-20612020000100250Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2020-05-29T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Proteomic analysis of the inhibitory effect of chitosan on Penicillium expansum |
title |
Proteomic analysis of the inhibitory effect of chitosan on Penicillium expansum |
spellingShingle |
Proteomic analysis of the inhibitory effect of chitosan on Penicillium expansum LI,Mingyan chitosan Penicillium expansum antifungal activity proteomics |
title_short |
Proteomic analysis of the inhibitory effect of chitosan on Penicillium expansum |
title_full |
Proteomic analysis of the inhibitory effect of chitosan on Penicillium expansum |
title_fullStr |
Proteomic analysis of the inhibitory effect of chitosan on Penicillium expansum |
title_full_unstemmed |
Proteomic analysis of the inhibitory effect of chitosan on Penicillium expansum |
title_sort |
Proteomic analysis of the inhibitory effect of chitosan on Penicillium expansum |
author |
LI,Mingyan |
author_facet |
LI,Mingyan CHEN,Chi XIA,Xiaoshuang GARBA,Betchem SHANG,Linlin WANG,Yun |
author_role |
author |
author2 |
CHEN,Chi XIA,Xiaoshuang GARBA,Betchem SHANG,Linlin WANG,Yun |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
LI,Mingyan CHEN,Chi XIA,Xiaoshuang GARBA,Betchem SHANG,Linlin WANG,Yun |
dc.subject.por.fl_str_mv |
chitosan Penicillium expansum antifungal activity proteomics |
topic |
chitosan Penicillium expansum antifungal activity proteomics |
description |
Abstract The antimicrobial effect of chitosan on Penicillium expansum, a major postharvest pathogen of pome fruit, and the possible mechanisms involved in its effect were examined in this study. Chitosan strongly inhibited spore germination and hyphal growth of P. expansum. Light microscopy and transmission electron microscopy observations revealed that chitosan also caused morphological changes in hyphae and conidia, such as abnormal branching and vacuolation. Proteomic changes in P. expansum after chitosan treatment were analyzed by two-dimensional electrophoresis (2-DE) and mass spectrometry (MS) analysis, and 26 proteins were ambiguously identified and categorized based on their putative biological function. Proteins related to DNA or protein biosynthesis, carbohydrate metabolism and energy production were decreased in relative protein abundance, while proteins involved in antibiotics resistance and defense response increased in relative protein abundance. Changes in abundance of these identified proteins were in accordance to the observed physiological and morphological changes of the fungi cells. Altogether, these experimental results provide a detailed illustration of the responses to chitosan in P. expansum, and widen our knowledge on the potential antifungal mechanisms of chitosan. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100250 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000100250 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.40418 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.40 n.1 2020 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126325618900992 |