Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000100151 |
Resumo: | Abstract Microbiological safety of fish is a concern of consumers, industries and regulatory agencies worldwide. Among the pathogenic microorganisms, Salmonella spp. is one of the main agents of foodborne diseases and should be absent in animal products. Rapid and accurate identification of pathogens in the supply chain is important for both quality assurance and tracking infectious agents within the chain. In this context, this study aimed to evaluate the equivalence of two rapid detection tests, as alternative methods to the conventional Salmonella detection method, as well as to verify the microbiological quality parameters of two commercially important fish species in the Amazon biome. The plate count of aerobic bacteria ranged from 7.76 x 104 to 8.71 x 107 CFU.g-1 for mesophiles and 1.70 x 106 to 4.27 x 108 CFU.g-1 for psychrotrophic whereas the maximum for this group of microorganisms in fresh fish is 106 CFU.g-1. Regarding the Staphylococcus count, the two species presented variations between 1.35 x 104 to 1.51 x 105 CFU.g-1. This represents unsatisfactory conditions of handling, storage and conservation of fish species. The immunoenzymatic and molecular methods have been shown to be reliable, fast and effective in the detection of Salmonella and for its high index of agreement with the conventional detection method. We also emphasize the convenience of multiplex PCR application due to the high sensitivity, specificity, speed and accuracy of Salmonella detection. |
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Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian AmazonBrachyplatystoma filamentosumIlisha amazonicamPCRTecra SalmonellaAbstract Microbiological safety of fish is a concern of consumers, industries and regulatory agencies worldwide. Among the pathogenic microorganisms, Salmonella spp. is one of the main agents of foodborne diseases and should be absent in animal products. Rapid and accurate identification of pathogens in the supply chain is important for both quality assurance and tracking infectious agents within the chain. In this context, this study aimed to evaluate the equivalence of two rapid detection tests, as alternative methods to the conventional Salmonella detection method, as well as to verify the microbiological quality parameters of two commercially important fish species in the Amazon biome. The plate count of aerobic bacteria ranged from 7.76 x 104 to 8.71 x 107 CFU.g-1 for mesophiles and 1.70 x 106 to 4.27 x 108 CFU.g-1 for psychrotrophic whereas the maximum for this group of microorganisms in fresh fish is 106 CFU.g-1. Regarding the Staphylococcus count, the two species presented variations between 1.35 x 104 to 1.51 x 105 CFU.g-1. This represents unsatisfactory conditions of handling, storage and conservation of fish species. The immunoenzymatic and molecular methods have been shown to be reliable, fast and effective in the detection of Salmonella and for its high index of agreement with the conventional detection method. We also emphasize the convenience of multiplex PCR application due to the high sensitivity, specificity, speed and accuracy of Salmonella detection.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2021-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000100151Food Science and Technology v.41 n.1 2021reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.38719info:eu-repo/semantics/openAccessSANTOS,Paula Hellayne Costa dosFIGUEIREDO,Hamilton M.SILVA,Luiza Helena Meller daSILVA,Rafaela Santos Oliveira daCARDOSO,Gabrielle Virginia FerreiraMORAES,Carina MartinsRODRIGUES,Antonio Manoel da Cruzeng2021-03-18T00:00:00Zoai:scielo:S0101-20612021000100151Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2021-03-18T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon |
title |
Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon |
spellingShingle |
Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon SANTOS,Paula Hellayne Costa dos Brachyplatystoma filamentosum Ilisha amazonica mPCR Tecra Salmonella |
title_short |
Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon |
title_full |
Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon |
title_fullStr |
Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon |
title_full_unstemmed |
Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon |
title_sort |
Evaluation of a rapid detection method of Salmonella in comparison with the culture method and microbiological quality in fish from the Brazilian Amazon |
author |
SANTOS,Paula Hellayne Costa dos |
author_facet |
SANTOS,Paula Hellayne Costa dos FIGUEIREDO,Hamilton M. SILVA,Luiza Helena Meller da SILVA,Rafaela Santos Oliveira da CARDOSO,Gabrielle Virginia Ferreira MORAES,Carina Martins RODRIGUES,Antonio Manoel da Cruz |
author_role |
author |
author2 |
FIGUEIREDO,Hamilton M. SILVA,Luiza Helena Meller da SILVA,Rafaela Santos Oliveira da CARDOSO,Gabrielle Virginia Ferreira MORAES,Carina Martins RODRIGUES,Antonio Manoel da Cruz |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
SANTOS,Paula Hellayne Costa dos FIGUEIREDO,Hamilton M. SILVA,Luiza Helena Meller da SILVA,Rafaela Santos Oliveira da CARDOSO,Gabrielle Virginia Ferreira MORAES,Carina Martins RODRIGUES,Antonio Manoel da Cruz |
dc.subject.por.fl_str_mv |
Brachyplatystoma filamentosum Ilisha amazonica mPCR Tecra Salmonella |
topic |
Brachyplatystoma filamentosum Ilisha amazonica mPCR Tecra Salmonella |
description |
Abstract Microbiological safety of fish is a concern of consumers, industries and regulatory agencies worldwide. Among the pathogenic microorganisms, Salmonella spp. is one of the main agents of foodborne diseases and should be absent in animal products. Rapid and accurate identification of pathogens in the supply chain is important for both quality assurance and tracking infectious agents within the chain. In this context, this study aimed to evaluate the equivalence of two rapid detection tests, as alternative methods to the conventional Salmonella detection method, as well as to verify the microbiological quality parameters of two commercially important fish species in the Amazon biome. The plate count of aerobic bacteria ranged from 7.76 x 104 to 8.71 x 107 CFU.g-1 for mesophiles and 1.70 x 106 to 4.27 x 108 CFU.g-1 for psychrotrophic whereas the maximum for this group of microorganisms in fresh fish is 106 CFU.g-1. Regarding the Staphylococcus count, the two species presented variations between 1.35 x 104 to 1.51 x 105 CFU.g-1. This represents unsatisfactory conditions of handling, storage and conservation of fish species. The immunoenzymatic and molecular methods have been shown to be reliable, fast and effective in the detection of Salmonella and for its high index of agreement with the conventional detection method. We also emphasize the convenience of multiplex PCR application due to the high sensitivity, specificity, speed and accuracy of Salmonella detection. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000100151 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000100151 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.38719 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.41 n.1 2021 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
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1752126328102977536 |