Polysaccharide isolated from Korean-style soy sauce activates macrophages via the MAPK and NK-κB pathways
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000400817 |
Resumo: | Abstract Soy sauce is a staple fermented food in Korea, however, there are relatively limited studies on soy sauce in Korea compared to that in Japan. In this study, the immuno-stimulating activities are compared in polysaccharides isolated from soy sauce in Korea and Japan. Crude polysaccharides was isolated, and which were named as Korean-style soy sauce (KSS-0) and Japanese-style soy sauce (JSS-0). Then, difference in cytokine production was compared in peritoneal macrophages, and KSS-0 showed higher cytokine production than JSS-0. The KSS-0 was purified into two polysaccharides, which were named as KSS-I and KSS-II. The polysaccharides consisted mainly galacturonic acid, xylose, and galactose. Out of the four polysaccharides isolated, KSS-II enhanced NO and cytokines production the most. Western blot analysis showed that KSS-II phosphorylated the MAPKs and nuclear factor NF-κB in RAW 264.7 cells in a dose-dependent manner. These results suggested that KSS-II activates macrophages via the MAPK and NF-κB pathways, and also induces an increase in the cytokine production. In conclusion, the polysaccharides isolated from Korean soy sauce have better immune-stimulating activities than those from Japan and may be used as potential functional foods. |
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Polysaccharide isolated from Korean-style soy sauce activates macrophages via the MAPK and NK-κB pathwayssoy saucepolysaccharidemacrophagecytokinesfunctional foodAbstract Soy sauce is a staple fermented food in Korea, however, there are relatively limited studies on soy sauce in Korea compared to that in Japan. In this study, the immuno-stimulating activities are compared in polysaccharides isolated from soy sauce in Korea and Japan. Crude polysaccharides was isolated, and which were named as Korean-style soy sauce (KSS-0) and Japanese-style soy sauce (JSS-0). Then, difference in cytokine production was compared in peritoneal macrophages, and KSS-0 showed higher cytokine production than JSS-0. The KSS-0 was purified into two polysaccharides, which were named as KSS-I and KSS-II. The polysaccharides consisted mainly galacturonic acid, xylose, and galactose. Out of the four polysaccharides isolated, KSS-II enhanced NO and cytokines production the most. Western blot analysis showed that KSS-II phosphorylated the MAPKs and nuclear factor NF-κB in RAW 264.7 cells in a dose-dependent manner. These results suggested that KSS-II activates macrophages via the MAPK and NF-κB pathways, and also induces an increase in the cytokine production. In conclusion, the polysaccharides isolated from Korean soy sauce have better immune-stimulating activities than those from Japan and may be used as potential functional foods.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2021-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000400817Food Science and Technology v.41 n.4 2021reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.06121info:eu-repo/semantics/openAccessSHIN,Kwang-SoonSEO,HaesunLEE,Sue JungSUNG,SooyunHWANG,Dahyuneng2021-12-10T00:00:00Zoai:scielo:S0101-20612021000400817Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2021-12-10T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Polysaccharide isolated from Korean-style soy sauce activates macrophages via the MAPK and NK-κB pathways |
title |
Polysaccharide isolated from Korean-style soy sauce activates macrophages via the MAPK and NK-κB pathways |
spellingShingle |
Polysaccharide isolated from Korean-style soy sauce activates macrophages via the MAPK and NK-κB pathways SHIN,Kwang-Soon soy sauce polysaccharide macrophage cytokines functional food |
title_short |
Polysaccharide isolated from Korean-style soy sauce activates macrophages via the MAPK and NK-κB pathways |
title_full |
Polysaccharide isolated from Korean-style soy sauce activates macrophages via the MAPK and NK-κB pathways |
title_fullStr |
Polysaccharide isolated from Korean-style soy sauce activates macrophages via the MAPK and NK-κB pathways |
title_full_unstemmed |
Polysaccharide isolated from Korean-style soy sauce activates macrophages via the MAPK and NK-κB pathways |
title_sort |
Polysaccharide isolated from Korean-style soy sauce activates macrophages via the MAPK and NK-κB pathways |
author |
SHIN,Kwang-Soon |
author_facet |
SHIN,Kwang-Soon SEO,Haesun LEE,Sue Jung SUNG,Sooyun HWANG,Dahyun |
author_role |
author |
author2 |
SEO,Haesun LEE,Sue Jung SUNG,Sooyun HWANG,Dahyun |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
SHIN,Kwang-Soon SEO,Haesun LEE,Sue Jung SUNG,Sooyun HWANG,Dahyun |
dc.subject.por.fl_str_mv |
soy sauce polysaccharide macrophage cytokines functional food |
topic |
soy sauce polysaccharide macrophage cytokines functional food |
description |
Abstract Soy sauce is a staple fermented food in Korea, however, there are relatively limited studies on soy sauce in Korea compared to that in Japan. In this study, the immuno-stimulating activities are compared in polysaccharides isolated from soy sauce in Korea and Japan. Crude polysaccharides was isolated, and which were named as Korean-style soy sauce (KSS-0) and Japanese-style soy sauce (JSS-0). Then, difference in cytokine production was compared in peritoneal macrophages, and KSS-0 showed higher cytokine production than JSS-0. The KSS-0 was purified into two polysaccharides, which were named as KSS-I and KSS-II. The polysaccharides consisted mainly galacturonic acid, xylose, and galactose. Out of the four polysaccharides isolated, KSS-II enhanced NO and cytokines production the most. Western blot analysis showed that KSS-II phosphorylated the MAPKs and nuclear factor NF-κB in RAW 264.7 cells in a dose-dependent manner. These results suggested that KSS-II activates macrophages via the MAPK and NF-κB pathways, and also induces an increase in the cytokine production. In conclusion, the polysaccharides isolated from Korean soy sauce have better immune-stimulating activities than those from Japan and may be used as potential functional foods. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000400817 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612021000400817 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.06121 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.41 n.4 2021 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
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1752126329901285376 |