Immunoelectron microscopy study of superficial skin nerves in drug-induced acute urticaria

Detalhes bibliográficos
Autor(a) principal: Criado,Paulo Ricardo
Data de Publicação: 2012
Outros Autores: Criado,Roberta Fachini Jardim, Takakura,Cleusa F.H., Pagliari,Carla, Sotto,Mirian Nacagami, Vasconcellos,Cidia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Anais brasileiros de dermatologia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962012000300004
Resumo: BACKGROUND: Few studies have evaluated the ultrastructure of the superficial skin nerves in urticaria. OBJECTIVE: The objective of this study was to describe findings in superficial skin nerves in cases of drug-induced acute urticaria. METHODS: Seven patients with drug-induced acute urticaria were included in the study. Skin biopsies were obtained from the urticarial lesion and from the apparently normal skin. The 14 fragments collected were processed for immunogold electron microscopy using single stains for antitryptase and anti-FXIIIa antibodies, as well as double immunogold labeling for both. RESULTS: Some sections showed mast cells in the process of degranulation. Following double immunogold staining, 10 nm (FXIIIa) and 15 nm (Tryptase) gold particles were found together throughout the granules in mast cells, indicating that tryptase and FXIIIa are located inside each one of the granules of these cells. Interestingly, we found strong evidence of the presence of tryptase and factor XIIIa in the superficial skin nerves of these patients, both in cases of urticarial lesions (wheals) and in the apparently normal skin. CONCLUSIONS: Tryptase and FXIIIa are present in the superficial nerves of the skin in drug-induced acute urticaria. This is the first report of tryptase and FXIIIa expression in the superficial skin nerves of patients with urticaria. Tryptase may be participating in neural activation in these patients, while FXIIIa may be present in the nerves to guarantee the functional integrity of structures.
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spelling Immunoelectron microscopy study of superficial skin nerves in drug-induced acute urticariafactor XIIIamast cellsneuronstryptasesurticariaBACKGROUND: Few studies have evaluated the ultrastructure of the superficial skin nerves in urticaria. OBJECTIVE: The objective of this study was to describe findings in superficial skin nerves in cases of drug-induced acute urticaria. METHODS: Seven patients with drug-induced acute urticaria were included in the study. Skin biopsies were obtained from the urticarial lesion and from the apparently normal skin. The 14 fragments collected were processed for immunogold electron microscopy using single stains for antitryptase and anti-FXIIIa antibodies, as well as double immunogold labeling for both. RESULTS: Some sections showed mast cells in the process of degranulation. Following double immunogold staining, 10 nm (FXIIIa) and 15 nm (Tryptase) gold particles were found together throughout the granules in mast cells, indicating that tryptase and FXIIIa are located inside each one of the granules of these cells. Interestingly, we found strong evidence of the presence of tryptase and factor XIIIa in the superficial skin nerves of these patients, both in cases of urticarial lesions (wheals) and in the apparently normal skin. CONCLUSIONS: Tryptase and FXIIIa are present in the superficial nerves of the skin in drug-induced acute urticaria. This is the first report of tryptase and FXIIIa expression in the superficial skin nerves of patients with urticaria. Tryptase may be participating in neural activation in these patients, while FXIIIa may be present in the nerves to guarantee the functional integrity of structures.Sociedade Brasileira de Dermatologia2012-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962012000300004Anais Brasileiros de Dermatologia v.87 n.3 2012reponame:Anais brasileiros de dermatologia (Online)instname:Sociedade Brasileira de Dermatologia (SBD)instacron:SBD10.1590/S0365-05962012000300004info:eu-repo/semantics/openAccessCriado,Paulo RicardoCriado,Roberta Fachini JardimTakakura,Cleusa F.H.Pagliari,CarlaSotto,Mirian NacagamiVasconcellos,Cidiaeng2012-06-15T00:00:00Zoai:scielo:S0365-05962012000300004Revistahttp://www.anaisdedermatologia.org.br/https://old.scielo.br/oai/scielo-oai.phpabd@sbd.org.br||revista@sbd.org.br1806-48410365-0596opendoar:2012-06-15T00:00Anais brasileiros de dermatologia (Online) - Sociedade Brasileira de Dermatologia (SBD)false
dc.title.none.fl_str_mv Immunoelectron microscopy study of superficial skin nerves in drug-induced acute urticaria
title Immunoelectron microscopy study of superficial skin nerves in drug-induced acute urticaria
spellingShingle Immunoelectron microscopy study of superficial skin nerves in drug-induced acute urticaria
Criado,Paulo Ricardo
factor XIIIa
mast cells
neurons
tryptases
urticaria
title_short Immunoelectron microscopy study of superficial skin nerves in drug-induced acute urticaria
title_full Immunoelectron microscopy study of superficial skin nerves in drug-induced acute urticaria
title_fullStr Immunoelectron microscopy study of superficial skin nerves in drug-induced acute urticaria
title_full_unstemmed Immunoelectron microscopy study of superficial skin nerves in drug-induced acute urticaria
title_sort Immunoelectron microscopy study of superficial skin nerves in drug-induced acute urticaria
author Criado,Paulo Ricardo
author_facet Criado,Paulo Ricardo
Criado,Roberta Fachini Jardim
Takakura,Cleusa F.H.
Pagliari,Carla
Sotto,Mirian Nacagami
Vasconcellos,Cidia
author_role author
author2 Criado,Roberta Fachini Jardim
Takakura,Cleusa F.H.
Pagliari,Carla
Sotto,Mirian Nacagami
Vasconcellos,Cidia
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Criado,Paulo Ricardo
Criado,Roberta Fachini Jardim
Takakura,Cleusa F.H.
Pagliari,Carla
Sotto,Mirian Nacagami
Vasconcellos,Cidia
dc.subject.por.fl_str_mv factor XIIIa
mast cells
neurons
tryptases
urticaria
topic factor XIIIa
mast cells
neurons
tryptases
urticaria
description BACKGROUND: Few studies have evaluated the ultrastructure of the superficial skin nerves in urticaria. OBJECTIVE: The objective of this study was to describe findings in superficial skin nerves in cases of drug-induced acute urticaria. METHODS: Seven patients with drug-induced acute urticaria were included in the study. Skin biopsies were obtained from the urticarial lesion and from the apparently normal skin. The 14 fragments collected were processed for immunogold electron microscopy using single stains for antitryptase and anti-FXIIIa antibodies, as well as double immunogold labeling for both. RESULTS: Some sections showed mast cells in the process of degranulation. Following double immunogold staining, 10 nm (FXIIIa) and 15 nm (Tryptase) gold particles were found together throughout the granules in mast cells, indicating that tryptase and FXIIIa are located inside each one of the granules of these cells. Interestingly, we found strong evidence of the presence of tryptase and factor XIIIa in the superficial skin nerves of these patients, both in cases of urticarial lesions (wheals) and in the apparently normal skin. CONCLUSIONS: Tryptase and FXIIIa are present in the superficial nerves of the skin in drug-induced acute urticaria. This is the first report of tryptase and FXIIIa expression in the superficial skin nerves of patients with urticaria. Tryptase may be participating in neural activation in these patients, while FXIIIa may be present in the nerves to guarantee the functional integrity of structures.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962012000300004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0365-05962012000300004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0365-05962012000300004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Dermatologia
publisher.none.fl_str_mv Sociedade Brasileira de Dermatologia
dc.source.none.fl_str_mv Anais Brasileiros de Dermatologia v.87 n.3 2012
reponame:Anais brasileiros de dermatologia (Online)
instname:Sociedade Brasileira de Dermatologia (SBD)
instacron:SBD
instname_str Sociedade Brasileira de Dermatologia (SBD)
instacron_str SBD
institution SBD
reponame_str Anais brasileiros de dermatologia (Online)
collection Anais brasileiros de dermatologia (Online)
repository.name.fl_str_mv Anais brasileiros de dermatologia (Online) - Sociedade Brasileira de Dermatologia (SBD)
repository.mail.fl_str_mv abd@sbd.org.br||revista@sbd.org.br
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