Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats

Detalhes bibliográficos
Autor(a) principal: Jamel,Márcio José
Data de Publicação: 2010
Outros Autores: Pereira,Lara de Paula Miranda, Mello,Natália Bernardes, Eleuthério,Elis Cristina Araújo, Schanaider,Alberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000100014
Resumo: PURPOSE: An experimental study was performed to investigate the use of protein carbonyl group as a specific biological marker for oxidative stress in a rat model of intestinal ischaemia-reperfusion. METHODS: Twenty four male Wistar rats were randomly distributed into three groups with eight animals each: Group 1 - Control group; Group 2 - Sham; Group 3 - Intestinal ischaemia by clamping ileal branches of the superior mesenteric artery for one hour, followed by another hour of reperfusion. Blood samples were taken in order to analyze the protein carbonyl level by Slot blotting assay. RESULTS: In group 3 a significant increase of protein carbonyl level was observed if compared to the homogenous levels of groups 1 and 2. CONCLUSION: From the results it may be concluded that the protein carbonylation may be used as a specific marker for measuring oxidative stress in rat intestinal reperfusion model.
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spelling Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in ratsOxidative StressFree RadicalsProtein CarbonylationIschemiaReperfusionIntestine SmallRatsPURPOSE: An experimental study was performed to investigate the use of protein carbonyl group as a specific biological marker for oxidative stress in a rat model of intestinal ischaemia-reperfusion. METHODS: Twenty four male Wistar rats were randomly distributed into three groups with eight animals each: Group 1 - Control group; Group 2 - Sham; Group 3 - Intestinal ischaemia by clamping ileal branches of the superior mesenteric artery for one hour, followed by another hour of reperfusion. Blood samples were taken in order to analyze the protein carbonyl level by Slot blotting assay. RESULTS: In group 3 a significant increase of protein carbonyl level was observed if compared to the homogenous levels of groups 1 and 2. CONCLUSION: From the results it may be concluded that the protein carbonylation may be used as a specific marker for measuring oxidative stress in rat intestinal reperfusion model.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2010-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000100014Acta Cirúrgica Brasileira v.25 n.1 2010reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502010000100014info:eu-repo/semantics/openAccessJamel,Márcio JoséPereira,Lara de Paula MirandaMello,Natália BernardesEleuthério,Elis Cristina AraújoSchanaider,Albertoeng2010-01-19T00:00:00Zoai:scielo:S0102-86502010000100014Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2010-01-19T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats
title Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats
spellingShingle Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats
Jamel,Márcio José
Oxidative Stress
Free Radicals
Protein Carbonylation
Ischemia
Reperfusion
Intestine Small
Rats
title_short Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats
title_full Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats
title_fullStr Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats
title_full_unstemmed Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats
title_sort Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats
author Jamel,Márcio José
author_facet Jamel,Márcio José
Pereira,Lara de Paula Miranda
Mello,Natália Bernardes
Eleuthério,Elis Cristina Araújo
Schanaider,Alberto
author_role author
author2 Pereira,Lara de Paula Miranda
Mello,Natália Bernardes
Eleuthério,Elis Cristina Araújo
Schanaider,Alberto
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Jamel,Márcio José
Pereira,Lara de Paula Miranda
Mello,Natália Bernardes
Eleuthério,Elis Cristina Araújo
Schanaider,Alberto
dc.subject.por.fl_str_mv Oxidative Stress
Free Radicals
Protein Carbonylation
Ischemia
Reperfusion
Intestine Small
Rats
topic Oxidative Stress
Free Radicals
Protein Carbonylation
Ischemia
Reperfusion
Intestine Small
Rats
description PURPOSE: An experimental study was performed to investigate the use of protein carbonyl group as a specific biological marker for oxidative stress in a rat model of intestinal ischaemia-reperfusion. METHODS: Twenty four male Wistar rats were randomly distributed into three groups with eight animals each: Group 1 - Control group; Group 2 - Sham; Group 3 - Intestinal ischaemia by clamping ileal branches of the superior mesenteric artery for one hour, followed by another hour of reperfusion. Blood samples were taken in order to analyze the protein carbonyl level by Slot blotting assay. RESULTS: In group 3 a significant increase of protein carbonyl level was observed if compared to the homogenous levels of groups 1 and 2. CONCLUSION: From the results it may be concluded that the protein carbonylation may be used as a specific marker for measuring oxidative stress in rat intestinal reperfusion model.
publishDate 2010
dc.date.none.fl_str_mv 2010-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000100014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000100014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502010000100014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.25 n.1 2010
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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