Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000100014 |
Resumo: | PURPOSE: An experimental study was performed to investigate the use of protein carbonyl group as a specific biological marker for oxidative stress in a rat model of intestinal ischaemia-reperfusion. METHODS: Twenty four male Wistar rats were randomly distributed into three groups with eight animals each: Group 1 - Control group; Group 2 - Sham; Group 3 - Intestinal ischaemia by clamping ileal branches of the superior mesenteric artery for one hour, followed by another hour of reperfusion. Blood samples were taken in order to analyze the protein carbonyl level by Slot blotting assay. RESULTS: In group 3 a significant increase of protein carbonyl level was observed if compared to the homogenous levels of groups 1 and 2. CONCLUSION: From the results it may be concluded that the protein carbonylation may be used as a specific marker for measuring oxidative stress in rat intestinal reperfusion model. |
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Acta Cirúrgica Brasileira (Online) |
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Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in ratsOxidative StressFree RadicalsProtein CarbonylationIschemiaReperfusionIntestine SmallRatsPURPOSE: An experimental study was performed to investigate the use of protein carbonyl group as a specific biological marker for oxidative stress in a rat model of intestinal ischaemia-reperfusion. METHODS: Twenty four male Wistar rats were randomly distributed into three groups with eight animals each: Group 1 - Control group; Group 2 - Sham; Group 3 - Intestinal ischaemia by clamping ileal branches of the superior mesenteric artery for one hour, followed by another hour of reperfusion. Blood samples were taken in order to analyze the protein carbonyl level by Slot blotting assay. RESULTS: In group 3 a significant increase of protein carbonyl level was observed if compared to the homogenous levels of groups 1 and 2. CONCLUSION: From the results it may be concluded that the protein carbonylation may be used as a specific marker for measuring oxidative stress in rat intestinal reperfusion model.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2010-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000100014Acta Cirúrgica Brasileira v.25 n.1 2010reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502010000100014info:eu-repo/semantics/openAccessJamel,Márcio JoséPereira,Lara de Paula MirandaMello,Natália BernardesEleuthério,Elis Cristina AraújoSchanaider,Albertoeng2010-01-19T00:00:00Zoai:scielo:S0102-86502010000100014Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2010-01-19T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats |
title |
Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats |
spellingShingle |
Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats Jamel,Márcio José Oxidative Stress Free Radicals Protein Carbonylation Ischemia Reperfusion Intestine Small Rats |
title_short |
Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats |
title_full |
Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats |
title_fullStr |
Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats |
title_full_unstemmed |
Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats |
title_sort |
Blood carbonyl protein measurement as a specific oxidative stress biomarker after intestinal reperfusion in rats |
author |
Jamel,Márcio José |
author_facet |
Jamel,Márcio José Pereira,Lara de Paula Miranda Mello,Natália Bernardes Eleuthério,Elis Cristina Araújo Schanaider,Alberto |
author_role |
author |
author2 |
Pereira,Lara de Paula Miranda Mello,Natália Bernardes Eleuthério,Elis Cristina Araújo Schanaider,Alberto |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Jamel,Márcio José Pereira,Lara de Paula Miranda Mello,Natália Bernardes Eleuthério,Elis Cristina Araújo Schanaider,Alberto |
dc.subject.por.fl_str_mv |
Oxidative Stress Free Radicals Protein Carbonylation Ischemia Reperfusion Intestine Small Rats |
topic |
Oxidative Stress Free Radicals Protein Carbonylation Ischemia Reperfusion Intestine Small Rats |
description |
PURPOSE: An experimental study was performed to investigate the use of protein carbonyl group as a specific biological marker for oxidative stress in a rat model of intestinal ischaemia-reperfusion. METHODS: Twenty four male Wistar rats were randomly distributed into three groups with eight animals each: Group 1 - Control group; Group 2 - Sham; Group 3 - Intestinal ischaemia by clamping ileal branches of the superior mesenteric artery for one hour, followed by another hour of reperfusion. Blood samples were taken in order to analyze the protein carbonyl level by Slot blotting assay. RESULTS: In group 3 a significant increase of protein carbonyl level was observed if compared to the homogenous levels of groups 1 and 2. CONCLUSION: From the results it may be concluded that the protein carbonylation may be used as a specific marker for measuring oxidative stress in rat intestinal reperfusion model. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000100014 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502010000100014 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502010000100014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.25 n.1 2010 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126439916830720 |