Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats

Detalhes bibliográficos
Autor(a) principal: Frias Neto,Carlos Alberto da Silva
Data de Publicação: 2014
Outros Autores: Koike,Márcia Kiyomi, Saad,Karen Ruggeri, Saad,Paulo Fernandes, Montero,Edna Frasson de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001500017
Resumo: PURPOSE: To evaluate effects of ischemic preconditioning and Cilostazol on muscle ischemia-reperfusion injury. METHODS: Male Wistar rats were submitted to muscle ischemic and reperfusion injury (4h of the left common iliac artery occlusion followed by 1h of reperfusion). Five experimental groups were constituted: Control group (n=4); Ischemia-Reperfusion (IR, n=5); Ischemic preconditioning group (IP, n=6); Ischemia-Reperfusion group treated with cilostazol (IRCi, n=6) and Ischemic preconditioning group treated with cilostazol (IPCi, n=6). At the end, left gracile muscle was removed and embedded in paraffin. Histopathology, neutrophil infiltration, myocyte necrosis and edema were analyzed. RESULTS: When compared with the control group, IR group showed increased neutrophil infiltration, severe necrosis and edema. There was significant difference between myocytes necrosis of IR group and IP group. There was no difference between the histopathological changes between IP, IRCi and IPCi groups. CONCLUSIONS: The model of IR caused severe muscle injury in the rat hind limb and ischemic preconditioning has a protective effect, reducing myocyte necrosis, however, treatment with cilostazol and also the association between cilostazol and preconditioning has no protective effect on the skeletal muscle subjected to ischemia and reperfusion injury.
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spelling Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in ratsIschemic preconditioningMuscle injuryCilostazolRats PURPOSE: To evaluate effects of ischemic preconditioning and Cilostazol on muscle ischemia-reperfusion injury. METHODS: Male Wistar rats were submitted to muscle ischemic and reperfusion injury (4h of the left common iliac artery occlusion followed by 1h of reperfusion). Five experimental groups were constituted: Control group (n=4); Ischemia-Reperfusion (IR, n=5); Ischemic preconditioning group (IP, n=6); Ischemia-Reperfusion group treated with cilostazol (IRCi, n=6) and Ischemic preconditioning group treated with cilostazol (IPCi, n=6). At the end, left gracile muscle was removed and embedded in paraffin. Histopathology, neutrophil infiltration, myocyte necrosis and edema were analyzed. RESULTS: When compared with the control group, IR group showed increased neutrophil infiltration, severe necrosis and edema. There was significant difference between myocytes necrosis of IR group and IP group. There was no difference between the histopathological changes between IP, IRCi and IPCi groups. CONCLUSIONS: The model of IR caused severe muscle injury in the rat hind limb and ischemic preconditioning has a protective effect, reducing myocyte necrosis, however, treatment with cilostazol and also the association between cilostazol and preconditioning has no protective effect on the skeletal muscle subjected to ischemia and reperfusion injury. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001500017Acta Cirúrgica Brasileira v.29 suppl.3 2014reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502014001700004info:eu-repo/semantics/openAccessFrias Neto,Carlos Alberto da SilvaKoike,Márcia KiyomiSaad,Karen RuggeriSaad,Paulo FernandesMontero,Edna Frasson de Souzaeng2015-07-30T00:00:00Zoai:scielo:S0102-86502014001500017Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2015-07-30T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats
title Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats
spellingShingle Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats
Frias Neto,Carlos Alberto da Silva
Ischemic preconditioning
Muscle injury
Cilostazol
Rats
title_short Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats
title_full Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats
title_fullStr Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats
title_full_unstemmed Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats
title_sort Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats
author Frias Neto,Carlos Alberto da Silva
author_facet Frias Neto,Carlos Alberto da Silva
Koike,Márcia Kiyomi
Saad,Karen Ruggeri
Saad,Paulo Fernandes
Montero,Edna Frasson de Souza
author_role author
author2 Koike,Márcia Kiyomi
Saad,Karen Ruggeri
Saad,Paulo Fernandes
Montero,Edna Frasson de Souza
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Frias Neto,Carlos Alberto da Silva
Koike,Márcia Kiyomi
Saad,Karen Ruggeri
Saad,Paulo Fernandes
Montero,Edna Frasson de Souza
dc.subject.por.fl_str_mv Ischemic preconditioning
Muscle injury
Cilostazol
Rats
topic Ischemic preconditioning
Muscle injury
Cilostazol
Rats
description PURPOSE: To evaluate effects of ischemic preconditioning and Cilostazol on muscle ischemia-reperfusion injury. METHODS: Male Wistar rats were submitted to muscle ischemic and reperfusion injury (4h of the left common iliac artery occlusion followed by 1h of reperfusion). Five experimental groups were constituted: Control group (n=4); Ischemia-Reperfusion (IR, n=5); Ischemic preconditioning group (IP, n=6); Ischemia-Reperfusion group treated with cilostazol (IRCi, n=6) and Ischemic preconditioning group treated with cilostazol (IPCi, n=6). At the end, left gracile muscle was removed and embedded in paraffin. Histopathology, neutrophil infiltration, myocyte necrosis and edema were analyzed. RESULTS: When compared with the control group, IR group showed increased neutrophil infiltration, severe necrosis and edema. There was significant difference between myocytes necrosis of IR group and IP group. There was no difference between the histopathological changes between IP, IRCi and IPCi groups. CONCLUSIONS: The model of IR caused severe muscle injury in the rat hind limb and ischemic preconditioning has a protective effect, reducing myocyte necrosis, however, treatment with cilostazol and also the association between cilostazol and preconditioning has no protective effect on the skeletal muscle subjected to ischemia and reperfusion injury.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001500017
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001500017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502014001700004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.29 suppl.3 2014
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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